MLH fusion antibacterial peptide, preparation method and application thereof
A hybrid antimicrobial peptide and antimicrobial peptide technology, applied in the field of genetic engineering, can solve the problems of high cost of chemical synthesis, low antimicrobial peptide, and difficult extraction, etc., and achieve the effects of wide application prospect, improved safety, and simple operation.
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[0032] The present invention provides a method for preparing MLH fusion antimicrobial peptides, using housefly antimicrobial peptides, LL-37 and Helicobacter pylori peptides as parent peptides, using bioinformatics methods to analyze the amino acid sequence of MLH, and according to E. coli codons Preference the gene fragment encoding the hybrid gene MLH, use SOE-PCR technology to synthesize the desired target gene, connect it with the expression vector pET-32a(+), and transform it into the E. coli expression strain to construct the recombinant genetic engineering strain pET- 32a-MLH / BL21(DE3), the protein fusion expression was induced by IPTG, and finally the antibacterial activity of the obtained fusion expressed hybrid peptide was identified. The results showed that the fusion expressed hybrid peptide was effective against Staphylococcus aureus and Bacillus subtilis Both have obvious antibacterial effects.
[0033] See Figure 5 , The present invention discloses a preparation m...
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