East-Asia scorpion antibiotic peptide gene and preparation method and application

An antibacterial peptide, scorpion pincer technology, applied in the directions of microorganism-based methods, botanical equipment and methods, biochemical equipment and methods, etc., can solve problems that do not involve the antibacterial function of scorpion toxins.
CN101063102AActive Publication Date: 2007-10-31唐克煌
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
唐克煌
Publication Date
2007-10-31

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Abstract

The invention discloses a preparing method of east Asia Tityus antibiotic peptide gene and appliance, which comprises the following steps: restructuring bacillus coli Escherichia coli DH5a / BmKAMP1, CCTCC NO: M207036; constructing east Asia Tityus ioterium cell cDNA library; choosing PCR method; sieving positive colony of scorpion antibiotic peptide gene from ioterium cDNA library; sequence-analyzing coding trait of antibiotic peptide gene; assuring amino acid sequence of the antibiotic peptide gene; adopting chemosynthesis antibiotic peptide; possessing inhibition of diverse density for Gram's bacterium. This antibiotic peptide possesses specificity and high active, which can be used as antibacterial drugs.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, and the invention relates to a scorpion antimicrobial peptide and the scorpion antimicrobial peptide gene, and also relates to a preparation method of the East Asian scorpion toxin gene, and also relates to the application of the gene, specifically, the present invention relates to Isolation of a scorpion antimicrobial peptide gene; involving a scorpion antimicrobial peptide identification of antimicrobial spectrum: it has broad-spectrum resistance to Gram-positive bacteria, but has no effect on Gram-negative bacteria; involving point mutations, its antimicrobial peptide improves the antibacterial Resistance of Gram-negative bacteria; also involved scorpion antimicrobial peptides are effective against clinically isolated drug-resistant hemolytic staphylococci, and have potential value for the development of antibacterial drugs. Background technique

[0002] East Asian pince...

Claims

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