11 results about "Scorpion toxin" patented technology

The invention discloses an anti-EV71 virus activity buthus martensii toxin polypeptide as well as a preparation method and application thereof, and belongs to the technical field of antiviral preparations. A scorpion tail electricity-saving stimulation method is adopted to obtain crude scorpion venom, and centrifugation and freeze drying are performed to obtain scorpion venom freeze-dried powder; dissolving the scorpion toxin freeze-dried powder, filtering, removing sediment, carrying out ultrafiltration centrifugation, and separating to obtain the anti-EV71 virus activity scorpion toxin polypeptide. According to the present invention, the scorpion martensii toxin polypeptides S-I (lt; 3kDa) and S-II (3-10 kDa) obtained from the scorpion martensii have good EV71 virus resistance; under the same concentration, the relative molecular mass is lt; compared with the prior art, the scorpion margaritae toxin polypeptide with the molecular weight of 3kDa has better anti-EV71 virus activity, has no toxic effect on cells, has the potential of being used as an anti-EV71 virus drug, and provides a theoretical basis for biomedical research and clinical application of the scorpion margaritae toxin polypeptide.

The invention discloses a polypeptide composition for promoting skin desquamation and a preparation method and application thereof, the polypeptide composition comprises polypeptides and a polyol preservative, the polypeptides comprise a first polypeptide, a second polypeptide and a third polypeptide, and the first polypeptide is acetyl tetrapeptide-2; the second polypeptide comprises one or more of hyaluronic acid polypeptide, red scorpion toxin and palmitoyl tripeptide-5; and the third polypeptide is prepared from one or more of acetyl octapeptide-3, palmitoyl dipeptide-7, acetyl tetrapeptide-9 and acetyl tetrapeptide-11. The polypeptide composition can significantly up-regulate the gene expression of kallikrein through a synergistic effect and promote old cutin exfoliation; meanwhile, gene expression of metalloproteinase is inhibited, and light aging is delayed. The polypeptide composition disclosed by the invention can be prepared into various cosmetics and has multiple effects of promoting skin desquamation, resisting wrinkles and repairing barriers.

The present invention discloses the use of the East Asian scorpion toxin polypeptide Makatoxin‑3 or a pharmaceutical composition containing the same in the preparation of a drug for treating and / or preventing liver diseases caused by lipid accumulation in the liver, in particular, in the preparation of a drug for treating and / or preventing non-alcoholic fatty liver disease. The present invention finds that Makatoxin‑3, as a PPARα agonist, significantly activates the transcription of genes related to fatty acid β-oxidation, activates the transcription of genes related to fatty acid β-oxidation, promotes lipid oxidation and decomposition to inhibit lipid accumulation in the liver, and reduces inflammation and liver fibrosis. Therefore, Makatoxin‑3 can be used to prepare a drug for treating non-alcoholic fatty liver disease.

The invention discloses a medicine for treating diabetic retinopathy and application of scorpion venom heat-resistant synthetic peptide in treating the disease, belongs to the field of research and development of polypeptide medicines, and is used for treating or preventing the diabetic retinopathy (DR). Experimental results show that the scorpion venom heat-resistant synthetic peptide (SVHRSP) has the effect of preventing and treating diabetic retinopathy (DR) on a streptozotocin (STZ)-induced mouse experimental diabetes model, and can repress retinal function impairment caused by impairment of streptozotocin (STZ)-induced diabetic mouse retinal photoreceptor cells and retinal nerve inner layer cells; and retina structure damage caused by thinning of the retina thickness of a diabetic mouse induced by STZ (streptozotocin) is inhibited.

The invention belongs to the technical field of component identification of beauty products, and particularly relates to an identification and detection method for adulteration components of fake botulinum toxin beauty products. According to the method, the sample to be detected is prepared into the test solution, the test solution is detected according to the high performance liquid chromatography-mass spectrometry, and the red scorpion toxin, the acetyl hexapeptide-1 and the snake venom-like tripeptide are taken as key indexes for judging fake and adulterated components of the botulinum toxin product, so that the safety and the effectiveness of the botulinum toxin product are ensured. According to the method, whether the sample is adulterated or not can be accurately judged only by using a standard reference substance of adulterated components when the method is established for the first time without using a reference substance in the subsequent conventional detection process, and the method for quantitatively analyzing the red scorpion toxin by using the liquid chromatography-tandem mass spectrometry is established for the first time. The method is easy and convenient to operate, good in repeatability, high in sensitivity, high in separation speed, high in detection flux and reliable in result, and the safety and effectiveness of botulinum toxin products are effectively guaranteed.

The application relates to the technical field of biological medicine engineering, and provides a preparation method and application of a scorpion toxin-derived transmembrane polypeptide. The OpiCalcin1 polypeptide is optimized in a truncation mode, two polypeptides Opi1 17-33 CSKKCKRRGTNPEKRCR and Opi1 23-33 RRGTNPEKRCR with excellent transmembrane function and low cytotoxicity are provided. Through experiment verification, the two cell transmembrane peptides have excellent transmembrane effect and low cytotoxicity, meanwhile, the defects of time-consuming and laborious in synthesizing the complete sequence of scorpion toxin OpiCalcin1 are overcome, the cell transmembrane peptides can be used for preparing drug, nuclear localization sequence and probe transport carriers, and the targeting efficiency is improved. Compared with traditional scorpion toxin medicine, the synthesis of the scorpion toxin to explore the transmembrane activity provides a new selection for the drug carrier.

The invention discloses a polypeptide kallikrein accelerant and application thereof, and belongs to the technical field of cosmetics, the polypeptide kallikrein accelerant comprises a polypeptide compound, a preservative and water, the polypeptide compound is at least one of red scorpion toxin, acetyl tetrapeptide-2, acetyl tetrapeptide-3, hexapeptide-11, hyaluronic acid polypeptide, palmitoyl tripeptide-5 and acetyl octapeptide-3, and the preservative is a mixture of glycerol, ethylhexylglycerol and 1, 2-ethylene glycol. The polypeptide kallikrein accelerant provided by the invention not only can promote skin desquamation by activating KLKs, but also can delay skin aging by inhibiting MMPs gene expression, and can be used for developing products with skin renewing and anti-aging dual effects.

The invention belongs to the field of biological medicine technology and food, and discloses application of scorpion toxin in preparation of weight-reducing medicine, and the scorpion toxin can be applied to preparation of the weight-reducing medicine, preparation of products for controlling fat accumulation in organisms, preparation of functional food for weight management and preparation of products for reducing the weight of the organisms. According to the application disclosed by the invention, the scorpion toxin can be used for reducing the body weight of an organism, reducing triglyceride and total cholesterol levels in serum, effectively relieving obesity and improving metabolic conditions such as glucose tolerance and insulin resistance, a new thought is provided for preventing and treating obesity and metabolic complications thereof, and the scorpion toxin has a wide clinical application prospect.

The application discloses a polypeptide kallikrein promoter and application thereof, and belongs to the technical field of cosmetics. The polypeptide kallikrein promoter comprises a polypeptide compound, a preservative and water. The polypeptide compound is at least one of red scorpion toxin, acetyl tetrapeptide-2, acetyl tetrapeptide-3, hexapeptide-11, hyaluronic acid polypeptide, palmitoyl tripeptide-5 and acetyl octapeptide-3. The preservative is a mixture of glycerol, ethylhexyl glycerin and 1,2-ethanediol. The polypeptide kallikrein promoter can not only promote skin desquamation by activating KLKs, but also delay skin aging by inhibiting MMPs gene expression, and can be used for developing products with skin renewal and anti-aging dual effects.

The invention discloses an anti-wrinkle firming micro-nano essence lotion and a preparation method thereof. The micro-nano essence lotion comprises the following components in parts by weight: 0.005-0.1 parts of ginseng tissue culture, 0.1-5 parts of PEG / PPG-70 / 30 tocopherol ether, 0.1-1 parts of hydrogenated lecithin, 3-12 parts of emollient oil, 3-15 parts of moisturizer, 0.0001-0.01 parts of red scorpion toxin peptide, 0.1-3 parts of ectoine, 0.05-2 parts of immortal grass extract, 0.01-2 parts of ergothioneine, 0.001-0.1 parts of acetyl hexapeptide-8, 0.001-0.1 parts of dipeptide diaminobutyryl benzylamide diacetate, 0.1-2 parts of hydrolyzed collagen, and 50-85 parts of deionized water. The present invention uses the synergistic effect of multiple anti-wrinkle and firming ingredients to combine immediate anti-wrinkle with long-term anti-wrinkle. Multiple effective moisturizing ingredients deeply replenish water and retain water on the surface. Long-term use can achieve the effects of lifting and firming, improving skin texture, brightening skin, and replenishing and moisturizing.

The invention relates to a scorpion toxin mutant ChTX-Q18W and an application of the scorpion toxin mutant ChTX-Q18W in preparation of an anti-epileptic medicine. Specifically, the invention provides a scorpion toxin mutant ChTX-Q18W and an active fragment thereof, or a pharmaceutically acceptable salt thereof, and the scorpion toxin mutant ChTX-Q18W and the active fragment thereof are used for preparing a preparation or a composition for treating and / or preventing epilepsy. The scorpion toxin mutant comprises an amino acid sequence as shown in SEQ ID NO: 2; or the scorpion toxin mutant has at least 50% identity with the amino acid sequence as shown in SEQ ID NO: 2 on the basis of keeping the 18th amino acid residue as W. It is proved for the first time that ChTX-Q18W can effectively prevent and / or treat epilepsy symptoms by measuring the influence of ChTX-Q18W on recurrent convulsion behaviors of a mouse PTZ convulsion model, and the ChTX-Q18W has a good clinical application prospect.