49 results about "Scorpion toxin" patented technology

The invention belongs to the field of medical biology, and particularly relates to a production method for recombining scorpion toxin protein AGAP. The method comprises the following steps: firstly, obtaining SUMO-AGAP fusion genes with His6 labels by using overlapping PCR technology; secondly, constructing a recombinant expression vector containing the SUMO-AGAP fusion genes; thirdly, transferring the constructed expression vector to a host cell to express; and finally, cracking the cell by using a physical method or a chemical agent, extracting recombinant fusion protein and His purified fusion protein to obtain pure SUMO-AGAP fusion protein, carrying out enzyme cutting reaction, releasing AGAP, and further purifying the fusion protein to obtain the purified AGAP protein. The method greatly improves the yield, saves the cost, and is simple and convenient to operate; and the AGAP produced by the method has broad biologic activity, can restrain the proliferation of various tumor cells, and simultaneously has analgesia effect.

The invention discloses a method for producing recombinant scorpion toxin protein by adopting a silkworm as a parasitifer, which mainly comprises the following steps: adopting a scorpion cDNA as a template, adopting 5'-ACGTAGATCTATGGATGGATATATAAG-3' containing BglII enzyme cutting site as a forward primer and adopting 5'-ACGTAGATCT TTACTTTTTTCCAC-3' containing the BglII enzyme cutting site as a reverse primer to carry out PCR gene amplification reaction in 32 cycles to obtain a scorpion toxin gene; digesting pCR2.1-scorpion toxin by restriction endonuclease BglII to obtain a scorpion toxin gene fragment; cloning the scorpion toxin gene fragment in a baculovirus transfer vector pAcGP67b to obtain a recombinant pAcGP67b-scorpion toxin gene, carrying out homologous recombination with baculovirus BmNPV DNA of a silkworm in an insect cell by cotransfection to produce a recombinant virus; expressing in a silkworm body and purifying the recombinant scorpion toxin. Most of the recombinant scorpion toxin protein expressed by a silkworm larva is retained in a fat body of the silkworm; the scorpion toxin is purified from the fat body by an affinity chromatography; and the recombinant scorpion toxin has obvious biological toxicity, is suitable for the requirement of a biopharmaceutical industrialization level and has wide application prospect in the field of future medical and clinic treatment.

The invention discloses a polynucleotide sequence of optimized coding Androctonus australis neurotoxin polypeptide AaIT. The polynucleotide sequence is suitable for the transcription expression in fungi. The invention also discloses a fungus strain capable of expressing Androctonus australis neurotoxin polypeptide AaIT as well as a preparation used to control insects, wherein the preparation does not do harm to the environment and other animals and plants except insects, thereby belonging to an environment-friendly preparation. The invention also discloses a promoter used to guide the specific expression of target gene in insect hemocoel. The invention combines well the characteristics of fungus cell that fungus cell can infect insects and parasitize in the bodies of insects and the toxicity of insect toxin polypeptide for the first time, thereby greatly increasing the insect killing efficiency of the fungi.

The invention discloses scorpion toxin antiviral polypeptide and a use of the same. Scorpion toxin antivirotic polypeptide (AVP-W2) is obtained through a molecular biological and chemical synthesis method, and then the antiviral activity of the AVP-W2 on measles virus (MeV) and human immunodeficiency virus (HIV) is measured through adopting an antibody neutralization method; moreover, the measurement shows that the AVP-W2 can effectively inhibit virus infection at a low concentration. The AVP-W2 has a tremendous prospect in making applied medicine which can cure or prevent a disease caused by virus. Moreover, the antiviral peptide of the invention has high activity on MeV and HIV and a simple method, and can be developed as antiviral medicine.

The invention provides an antimicrobial peptide derived from scorpion venom as well as a preparation method and application of the antimicrobial peptide. The antimicrobial peptide RLL has a sequence shown as SEQ ID No.1. A peptide RSN is obtained by intercepting 14 amino acids in the middle of the scorpion toxin, negatively charged aspartic acid in the peptide RSN is replaced with positively charged arginine, and serine, glycine and asparagine in a peptide chain are replaced with leucine to obtain the antimicrobial peptide RLL; and the antimicrobial peptide RLL has very low hemolytic activityand eukaryotic cytotoxicity, the antimicrobial peptide cannot cause 10% erythrocyte hemolysis at a concentration of 128 [mu]mol/L, the survival rate of mouse macrophages RAW 264.7 reaches 80% or more,and the antimicrobial peptide has the development potential of becoming an antibiotic substitute.

This invention relates to an isolated nucleic acid fragment encoding scorpion toxins that are K-channel agonists. The invention also relates to the construction of a chimeric gene encoding all or a portion of the K-channel agonists, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the K-channel agonists in a transformed host cell.

The invention relates to a recombinant plasmid of a specific ligand of a BK channel and a recombinant expression method thereof. The recombinant plasmid of the ligand is a base sequence shown in SEQ ID NO:1. The invention provides establishment of an in-vitro expression vector pGEX-4T-3 and technical parameters of a MarTX in-vitro expression method. The short chain scorpion toxin MarTX obtained in the invention is successfully expressed in a pGEX-4T-3 and the molecular weight and bioactivity of the short chain scorpion toxin MarTX are almost the same as those of the natural MarTX; the recombinant MarTX is capable of selectively blocking the BK channel (alpha+beta4) and partially inhibiting the electric current of an mKv1.3 channel but has no obvious modulating effect on hKv4.2 and hKv3.1a.

The invention discloses freeze-dried powder for delaying senescence. The freeze-dried powder for delaying senescence is prepared from the following components in percentage by mass: 81-85% of water, 6-8% of mannite, 4-6% of dipeptide diaminobutyroyl benzylamide diacetate, 2.5-3.5% of red scorpion toxin, 0.08-0.12% of glycerin, 0.7-0.9% of trehalose, 0.3-0.5% of aureobasidium pullulans polysaccharide, 0.2-0.4% of glycine, 0.08-0.12% of sodium hyaluronate and 0.1-0.15% of natulin tensilk, and the sum of the mass percentages of the components is 100%. By means of the freeze-dried powder for delaying senescence, facial skin hydropenia conditions are effectively improved, skin tension is improved, facial skin elasticity is restored, and false wrinkles are reduced; by using the freeze-dried powder for delaying senescence continuously, skin can be fundamentally changed, and is bright, smooth, plumper, glossy and full of elasticity, wrinkles almost cannot be seen on the face or around the eyes, and a natural state of the health skin is presented.

The invention discloses scorpion toxin active polypeptide HsTx1 capable of improving cerebral ischemia and hypoxia and a preparation method and application of the scorpion toxin active polypeptide HsTx1. An amino acid sequence of the scorpion toxin active polypeptide HsTx1 capable of improving cerebral ischemia and hypoxia is AGKKERAGSRRTKIVMLKCIREHGH. According to the scorpion toxin active polypeptide HsTx1 capable of improving cerebral ischemia and hypoxia and the preparation method and the application of the scorpion toxin active polypeptide HsTx1, the purified and obtained rainforest scorpion toxin polypeptide HsTx1 shows strong anti-cerebral ischemia and hypoxia activity and activity of improvement of motor dysfunctions in a cerebral ischemia and hypoxia model (MCAO) experiment, and it is indicated that the 'scorpion toxin active polypeptide HsTx1 capable of improving cerebral ischemia and hypoxia' has a great prospect of medical application; and the scorpion toxin active polypeptide HsTx1 capable of improving cerebral ischemia and hypoxia has the beneficial characteristics of strong activities of efficiently improving cerebral ischemia and hypoxia and the motor dysfunctions.

The invention relates to a buthotoxin spray, which is suitable for people suffering from pain which is caused by cervical spondilopathy, periarthritis of shoulder, neurogenic headache, rheumatism, trigeminal neuralgia, arthralgia, lumbus ache and leg pain, etc.; the spray can obviously release a plurality of pains without toxic and side effect. The prescription of the buthotoxin spray for preparing 1000ml spray has 0.02-0.05g of buthotoxin, 0.02-0.10g of bufotoxin, 100-130ml of laurocapram with a concentration of 99 percent and 870-900ml of alcohol with a concentration of 55 percent. The preparation method is that buthotoxin is soaked in alcohol with a concentration of 55 percent for 1 to 2 days, then filtered and impurities are removed, and the remained solution is to reserve; bufotoxin is soaked by defined amount of alcohol with a concentration of 55 percent for 15 to 20 days; the dissolved substance is filtered and degermed after the bufotoxin is completely dissolved; then all materials are fully mixed according to the proportion and are packed into spray bottles (cans).

The invention discloses a method for fixing the scorpion toxin protein in the silkworm BmNPV polyhedrosis crystal, comprising the following steps: (1) cloning the scorpion toxin protein BmK gene; (2) digesting the pCR2.1-BmK gene by the restriction endonucleases BamHI and HindIII to obtain a BmK gene fragment, cloning the BmK gene fragment into the baculovirus transfer vector pBlueBacHisa to obtain the recombinant transfer vector, cotransferctign the recombinant transfer vector and the wild nuclear nuclear polyhedrosis virus BmNPVDNA of the silkworm into the silkworm culture cells, and generating the recombinant baculovirus containing the scorpion toxin protein genes; and (3) inoculating the recombinant baculovirus containing the scorpion toxin protein gene and the wild nuclear nuclear polyhedrosis virus BmNPVDNA agaist the silkworm culture cells, infecting for 3 days at 27 DEG C, and collecting the silkworm culture cells by centrifuging. The method overcomes the defect that the scorpion toxin protein is easy to lose and creates a condition for developing the scorpion toxin protein used as the biological insecticide.

The invention discloses an extracting and purifying process for scorpion toxins. The extracting and purifying process comprises the following steps: performing anesthesia disposal on scorpions throughalcohol or ethyl ether, freezing to take a sample, adding alcohol into the sample, grinding the sample into slurry, and centrifuging the slurry to obtain liquid supernatant; adding absolute ethyl alcohol into the liquid supernatant, uniformly mixing, and centrifuging to take upper-layer protein fluid; adding protease K into the upper-layer protein fluid, adding absolute ethyl alcohol of equal volume and a statured sodium chloride solution after treatment is ended, and centrifuging to take an upper-layer solution after uniformly mixing; adding a sodium sulfate aqueous solution into the upper-layer solution, adding absolute ethyl alcohol after uniformly mixing, and centrifuging to collect the upper-layer solution after uniformly mixing; and freeze-drying the upper-layer solution to preparefreeze-dried powder. The extracting and purifying process adopts analytical chemical means to directly extract scorpion toxins from pedipalps, and can avoid waste caused by incomplete extracting of the existing method.

The invention relates to the technical field of medicinal liquor, and in particular relates to snake-scorpion pain-relieving and wind-dispelling liquor and a preparation method thereof. The liquor comprises medicinal cyathula root, angelica pubescens, cortex acanthopanacis, cortex erythrinae, cinnamon, ligusticum wallichii, Chinese angelica, radix codonopsis, radix clematidis, divaricate saposhnikovia root, eucommia, monkshood, tripterygium glycosides, prepred nux vomica, liquorice, snake venom polypeptide, scorpion venom polypeptide, honey and distilled spirit. The medicinal liquor is prepared by adopting an infiltration method, the preparation method is simple, the liquor is convenient to take and tasted comfortably, and especially can be used for effectively treating rheumatism arthralgia, tendon spasm and other diseases.

The invention discloses youth activating concentrate for delaying skin senescence. The youth activating concentrate for delaying skin senescence is characterized by being prepared from the following components in percentage by mass: 91-95% of water, 2-4% of agave americana leaf, 1-3% of propylene glycol, 0.5-1.5% of red scorpion toxin, 0.2-0.8% of assistant, 0.08-0.12% of sodium hyaluronate and 0.08-0.12% of natulin tensilk, and the sum of the mass percentages of the components is 100%. By means of the youth activating concentrate for delaying skin senescence, facial skin hydropenia conditionsare effectively improved, skin tension is improved, facial skin elasticity is restored, and false wrinkles are reduced; by using the youth activating concentrate for delaying skin senescence continuously, skin can be fundamentally changed, and is bright, smooth, plumper, glossy and full of elasticity, wrinkles almost cannot be seen on the face or around the eyes, and a natural state of the healthskin is presented.

The invention discloses an arginine/lysine polypeptide microcapsule inclusion as well as a preparation method and application thereof. The microcapsule inclusion is prepared from the following raw materials: compound arginine/lysine polypeptide, glycerol, a polyglyceryl nonionic surfactant, glucan and the balance of water, wherein the compound arginine/lysine polypeptide comprises red scorpion toxin and conotoxin peptide. According to the microcapsule inclusion, a polyglyceryl nonionic surfactant is adopted as a carrier material, so that the compound polypeptide can be spontaneously wrapped in a microcapsule bilayer, a high-stability microcapsule inclusion structure is formed, and the compound polypeptide can achieve the effects of resisting aging, wrinkles and oxidation. The preparation method of the microcapsule inclusion avoids the addition of excipients and plasticizers required by traditional liposome preparation, and is simple. The microcapsule inclusion can be conveniently and stably dispersed in different types of cosmetic substrates such as skin care emulsion, cream, face cleansing, essence, toning lotion and the like to be prepared into cosmetics or skin care products for application.

The invention discloses a chicken for pregnant women and nursing women and meat and soup thereof. The chicken for pregnant women and nursing women and the meat and the soup of the chicken are characterized in that fine snake toxin extracting solution, fine scorpion toxin extracting solution, fine centipede toxin extracting solution, fine bufotoxin extracting liquid and fine gecko extracting liquid are utilized, and the fine extracting liquid of rare traditional Chinese medicines, namely, cornua cervi pantotrichum and ginseng, are added; all the materials are scientifically and reasonably mixed, and the mixing ratio is strictly controlled, so that the toxin essences can coordinate and fit each other; the chicken for pregnant women and nursing women is used as a carrier of the toxins and the extracting solutions; the effects of the toxin essences are included into a chicken body of the chicken for pregnant women and nursing women and related meat and soup through absorbing and converting; the rare traditional Chinese medicines, namely, cornua cervi pantotrichum and ginseng, play the key roles. Therefore, the constitution and the immunity of women and children can be obviously improved; the intelligence development of the children can be prompted; the essence of Chinese medical science and modern medical theory are combined; the toxicology and function test results show that the children and the meat and soup thereof meet the specifications of State Food and Drug Administration on health food.