Lactobacillus reuteri SF-L-25 with helicobacter pylori inhibiting function and application thereof

A technology of SF-L-25 and Lactobacillus reuteri, applied in the field of microorganisms, can solve problems such as gastrointestinal flora imbalance, antibiotic gastrointestinal dysfunction, etc., achieve high safety, wide application value, and inhibit Helicobacter pylori The effect of growth

Pending Publication Date: 2021-03-02
SHANDONG SUNFLOWER BIOENGINEERING CO LTD
View PDF4 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Aiming at the problems existing in the prior art, the present invention provides a strain of Lactobacillus reuteri SF-L-25 with the function of inhibiting Helicobacter pylori and its application, effectively solving the

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Lactobacillus reuteri SF-L-25 with helicobacter pylori inhibiting function and application thereof
  • Lactobacillus reuteri SF-L-25 with helicobacter pylori inhibiting function and application thereof
  • Lactobacillus reuteri SF-L-25 with helicobacter pylori inhibiting function and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 Screening of Lactobacillus reuteri SF-L-25

[0041] ① Source of bacteria: healthy human gastric juice

[0042] ② Plate medium: beef extract 10.0g, yeast extract 5.0g, peptone 10.0g, grape 20.0g, K 2 HPO 4 2.0g, Sodium acetate 5.0g, MgSO 4 ·7H 2 O 0.2g, MnSO 4 ·4H 2 O0.05g, Tween 801.0g, triammonium citrate 2.0g, agar 15.0g, add distilled water to 1000ml.

[0043] ③Isolation of strains

[0044] The sample liquid was diluted ten-fold, and 100 μL of each gradient was evenly spread on the MRS solid plate medium, then taken out and placed in an anaerobic tank, and cultured overnight at 37°C. The next day, colonies of different shapes and sizes were found on the plate, and some characteristic colonies of lactic acid bacteria were picked for the second-generation streak purification treatment, and the plate was anaerobic activated for 1 day to obtain a culture, followed by strain identification.

[0045] ④ Determination of 16S rRNA sequence

[0046] The 16S ...

Embodiment 2

[0049] Embodiment 2 Determination of antibacterial ability of Lactobacillus reuteri SF-L-25

[0050] Lactobacillus reuteri SF-L-25 was activated for 2 generations, and the bacterial suspension was filtered through a 0.22 μm sterile filter to obtain the Lactobacillus reuteri SF-L-25 fermentation supernatant. The H.pylori bacterial suspension of activated 2 generations was adjusted to 10 8 CFU / mL, 100 μL was spread on Columbia blood plate, 150 μL Lactobacillus reuteri SF-L-25 fermentation supernatant was added to the Oxford cup, and the control group was added with the same amount of distilled water, and was cultured in a three-gas incubator 72h, determine the diameter of the inhibition zone (see figure 1 ). Do 1 control and 3 parallel experiments.

[0051] Table 1 Bacteriostatic ability of Lactobacillus reuteri SF-L-25

[0052] group Inhibition zone / (mm) control 0 Test group 1 12.92 Test group 2 12.38 Test group 3 12.15

[0053] The ...

Embodiment 3

[0054] Example 3 Determination of Adhesion Ability

[0055] Human gastric adenocarcinoma cells (AGS) were seeded in 96-well plates (2×10 4 Cells / well) were cultured overnight, and after the cells adhered to the wall, F-12 resuspended Helicobacter pylori and Lactobacillus reuteri SF-L-25 were added at a multiplicity of infection of 100 for 2 hours, respectively, and then 200 μL of urease reagent was added for 3 hours. Measure the absorbance at 550nm wavelength, and do 1 control and 3 parallel experiments.

[0056] Table 2 Determination of the ability of Lactobacillus reuteri SF-L-25 to inhibit the adhesion of Helicobacter pylori

[0057] group Adhesion rate / (%) control 100 Test group 1 69.62 Test group 2 70.38 Test group 3 69.25

[0058] The results showed that the adhesion rate of Helicobacter pylori to AGS decreased significantly after treatment with Lactobacillus reuteri SF-L-25.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to the technical field of microorganisms, in particular to lactobacillus reuteri SF-L-25 with a helicobacter pylori inhibiting function and application thereof. The lactobacillusreuteri SF-L-25 is preserved in the China General Microbiological Culture Collection Center on October 26, 2020, and the preservation number of the lactobacillus reuteri SF-L-25 is CGMCC No. 20956; and the lactobacillus reuteri is named as lactobacillus reuteri in a classified manner. The preservation address is No.3, Yard 1, West Beichen Road, Chaoyang District, Beijing; the lactobacillus reuteri SF-L-25 is separated and screened from gastric mucus of a healthy human body, is high in safety, has relatively strong stability, inhibits helicobacter pylori growth and urease activity, the problems of gastrointestinal dysfunction, gastrointestinal dysbacteriosis and other adverse reactions caused by long-term unreasonable use of antibiotics in the process of preventing and treating helicobacter pylori infection are effectively solved; meanwhile, the lactobacillus reuteri SF-L-25 has remarkable functional characteristics so that the lactobacillus reuteri SF-L-25 has wide application value in probiotic solid beverages.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a strain of Lactobacillus reuteri SF-L-25 with the function of inhibiting Helicobacter pylori and its application. Background technique [0002] The accelerated pace of life in modern society, high work pressure and unhealthy eating habits have exacerbated the incidence of gastrointestinal diseases. Among them, the incidence and mortality of gastric cancer have remained high for a long time, which greatly threatens people's health. Helicobacter pylori is recognized as the main pathogenic factor of chronic active gastritis, gastroduodenal ulcer, gastric mucosa-associated lymphoid tissue lymphoma, gastric cancer and other digestive system diseases. Helicobacter pylori is a unipolar, Bacteria with polyflagellates, blunt ends, and spiral-curved bacteria often have a typical spiral or arc shape on the surface of gastric mucosal epithelial cells. Eradication of Helicobacter pylo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/20A23L2/39A23L33/135A61K35/747A61P1/04A61P1/00A61P31/04C12R1/225
CPCA23L2/39A23L33/135A61K35/747A61P1/04A61P1/00A61P31/04A23V2002/00A23V2400/173A23V2200/3204A23V2250/032A23V2250/28A23V2250/5114
Inventor 夏越马明磊林伟王艳韩文立
Owner SHANDONG SUNFLOWER BIOENGINEERING CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap