29 results about "Urocaninase" patented technology

The present invention relates to increasing and / or maintaining nitrogen content in soil by administration of nitrogen sources particles coated with urease inhibitors. In one embodiment, urease inhibitors are dispersed in an improved organo liquid delivery system at concentration levels of 60-95%. In another embodiment, urease inhibitors were applied in a non-aqueous, organo liquid delivery system coating nitrogen sources particles utilizing simple blending equipment at temperatures of 20°-70° C. Another embodiment discloses dry, flowable nitrogen sources coated with a urease inhibitor which can be administered directly to the soil, to a dry natural and / or a manmade fertilizer or to a liquid fertilizer which provides for the reduction of nitrogen loss from the soil. In another embodiment, a composition of urea and <0.2% dimethyl sulfoxide improves the crush resistance of the urea particles.

The invention discloses a dialysate regeneration system and dialysis equipment, the dialysate regeneration system comprises a waste liquid passage, a slow flow box, an electromagnetic valve, a reactor and an adsorption mechanism, the waste liquid passage is in through connection with the slow flow box, the slow flow box is connected with the reactor through a pipeline, and the pipeline is provided with the electromagnetic valve. The fixing amount of a urease layer is increased through the treatment film with the three cambered surfaces, the urea decomposition efficiency is improved, the diffusion path of toxins on the treatment film is shortened, meanwhile, the treatment film with the cambered surface structure can guide dialysis waste liquid entering the reactor to generate local turbulence, and the toxins are prevented from being accumulated on the surface of the treatment film; the dialyzer is arranged, the micro horse is started to drive the hollow fiber to rotate, the material exchange efficiency of blood and dialysate is improved, and the situation that protein and cells are deposited on the surface of the hollow fiber, and the service life of the hollow fiber is shortened is avoided.

A chitosan-heparin nanomotor and a method for producing same are disclosed. A STING agonist-encapsulated urease-based chitosan-heparin nanomotor delivers the STING agonist directly to bladder mucosal cells in the bladder, and thus can induce an immune response.

The invention relates to a 14C urea pharmaceutical composition for helicobacter pylori diagnosis and application of the 14C urea pharmaceutical composition. The 14C urea pharmaceutical composition comprises 14C urea, 12C urea, citric acid, malic acid and mannitol. According to the invention, the 12C urea and the 14C urea are added together, so that the radioactivity can be ensured, the stability of the medicine can be improved, and the auto-radiolysis of 14C can be reduced. Besides, citric acid, malic acid and mannitol have a remarkable synergistic effect in the aspect of improving the radioactive activity and radiochemical purity of the composition, the stability of the 14C urea pharmaceutical composition can be improved, meanwhile, the activity of urease generated by helicobacter pylori can be improved, and the detection sensitivity and detection efficiency are further guaranteed.

The invention provides a phthalocyanine compound as well as a preparation method and application thereof, and relates to the technical field of medicinal chemistry. The structure of the phthalocyanine compound is A-X-B. In the formula, A is a structure containing phthalocyanine and salts thereof; x is a divalent organic structure which enables A and B to be bonded through a chemical bond; and B contains a first polypeptide structure which specifically binds and / or recognizes urease. The phthalocyanine compound disclosed by the invention combines the effects of a photosensitizer and targeting, and has good targeting and antibacterial activity on helicobacter pylori.

The application discloses an auxiliary diagnosis method for red bayberry debilitation disease, which comprises collecting healthy red bayberry growth soil and suspected diseased red bayberry growth soil in the same area, and respectively determining urease activity of the soil; and comparing and analyzing urease activity determination results of the healthy red bayberry growth soil and the suspected diseased red bayberry growth soil. If urease activity of the suspected diseased red bayberry growth soil is significantly lower than that of the healthy red bayberry, it is indicated that the suspected diseased red bayberry is likely to have the debilitation disease; if there is no significant difference between urease activity of the suspected diseased red bayberry growth soil and that of the healthy red bayberry growth soil, it is indicated that the suspected diseased red bayberry does not have the debilitation disease. The application can assist in diagnosing the red bayberry debilitation disease, avoids subjective misjudgment of personnel observation diagnosis, and the method is simple, rapid and low in detection cost. In addition, the diagnosis result of the method can provide a reference for further orchard soil treatment and debilitation disease prevention of fruit farmers.

A liquid fertilizer additive solution composition and methods of making thereof.

The invention relates to the technical field of pathogenic microorganism detection, and discloses a helicobacter pylori detection kit based on a urease method and a preparation method, the helicobacter pylori detection kit comprises a detection sleeve card, detection test paper and a tartar sampling label, the test paper is soaked in a reaction solution and a buffer solution in the preparation process of the detection test paper, the pH of the buffer solution is 6.5-7.0, and the tartar sampling label is used for sampling tartar. The buffer solution comprises monopotassium phosphate and dipotassium phosphate, the final concentration of the monopotassium phosphate is 14-30 g / L, and the final concentration of the dipotassium phosphate is 1.8-3.6 g / L. The method can solve the problems of high false positive rate of detection results, poor accuracy, slow reagent response speed and the like caused by complex oral environment (such as diet residue and non-specific bacterial interference) and lack of an effective control system when a tartar sample is used for detecting helicobacter pylori in the prior art.

The invention discloses a durable anti-seepage repairing material and a preparation method thereof, and belongs to the technical field of anti-seepage plugging materials. The anti-seepage material is prepared from a sulphoaluminate base material, calcium aluminate-chitosan microcapsules, an enzymatic deposition material, an emulsion and a functional auxiliary agent; the sulphoaluminate base material is prepared from sulphoaluminate, calcium carbonate, anhydrite and silica fume; according to the calcium aluminate-chitosan microcapsule, a core material is calcium aluminate, and a wall material is composed of chitosan and methyl cellulose; the enzymatic deposition material is composed of urease and bacterial cellulose nanofibrils and urea microcapsules, the core material of the urea microcapsules is urea, and the wall material of the urea microcapsules is sodium alginate; the emulsion is prepared from epoxidized soybean oil acrylate, chitosan nanocrystals, bacterial cellulose nanofibrils and a photoinitiator; the functional additive is composed of a polycarboxylate superplasticizer, a retarder and a defoaming agent. The anti-seepage repair material prepared by the invention has excellent toughness, and can be repaired and healed after cracking.

Described are compositions (“suspensions”) that contain dicyandiamide (DCD) suspended in particle form in an liquid medium, with optional urease inhibitor, biostimulator, or micronutrient also contained (e.g., dissolved) in the liquid medium, as well as methods of preparing the compositions, methods of using the compositions, and fertilizer products that contain the compositions.

A rapid detection method of urea concentration based on photoelectrochemical analysis and application thereof, the rapid detection method of urea concentration based on photoelectrochemical analysis comprises the following steps: (1) preparing a CDs / C3N4 / ITO electrode; (2) adding urease solution, urease reaction buffer and urea solution with different concentrations into a 96-well plate and incubating; (3) preparing a CDs / C3N4 / ITO electrode adsorbed with methylene blue; (4) photoelectrochemically detecting the urea concentration; (5) drawing a standard curve control diagram; and (6) detecting the concentration of urea solution with unknown concentration. The detection method is used for detecting the urea concentration in human body fluid or environmental water. The rapid detection method of urea concentration can be used to prepare a urea concentration detection sensor. The detection method has excellent selectivity, good repeatability and other advantages for instant detection of the urea concentration in human body fluid or environment.

The present invention relates to mixtures comprising a solid support comprising a urease inhibitor and another solid support comprising a nitrification inhibitor, such as 3,4-dimethylpyrazole phosphate (DMPP) or 2-(3,4-dimethyl-1H-pyrazol-1-yl)succinic acid (DMPSA), to a method for enhancing the nitrification inhibitory effect or for increasing the health of a plant using mixtures of at least one compound I and at least one compound II; to the use of mixtures comprising compounds I and II for increasing the health of a plant.

The utility model discloses a device for biologically solidifying construction waste, which relates to the technical field of constructional engineering and comprises a support, a humidity control maintenance bin is fixedly connected to the inner wall of the support, a solidifying mechanism is arranged on the support, a uniform spraying mechanism is arranged on the support, and the humidity control maintenance bin is fixedly connected to the inner wall of the support. By arranging the strain culture reactor, firstly, urease-producing bacteria are cultured in the strain culture reactor, a culture medium containing urea and calcium salt is supplied for proliferation, high-activity bacterial liquid is obtained, then to-be-cured building waste crushed aggregates such as waste bricks and stones and concrete particles are flatly laid in the humidity-control curing bin, and the humidity-control curing bin is used for curing the building waste. According to the mechanism, urea in a nutrient solution is promoted to be decomposed through urease generated by bacteria, carbonate ions are formed, then the carbonate ions are combined with calcium ions to precipitate calcium carbonate crystals, adjacent particles are cemented together, building waste stacks can be reinforced into blocks or roadbed materials without traditional cementing materials such as cement, the process is environmentally friendly, and the construction waste is recycled. And new engineering value is given to the construction waste.

A method and a composition, characterized as having enhanced inhibitory urease activities, having a diarylheptanoid compound having a first aryl moiety group coherently bonded to a linear heptane moiety and a second aryl moiety group described by the following formula:

The present invention relates to urease inhibitor compositions containing a tert-butylhydroquinone and optionally an additive component for enhancing the inhibitory properties of the tert-butylhydroquinone. The use of the composition in agriculture to increase nutrient uptake and inhibit urease activity is also described.

The invention discloses bacillus amyloliquefaciens YY-27 and application thereof, the bacillus amyloliquefaciens YY-27 is preserved in China Center for Type Culture Collection, the preservation number is CCTCC NO: M2025178, the preservation date is January 16, 2025, and the address is Wuhan University, Luojia Hill Road, Wuchang District, Wuhan City, Hubei Province; the bacillus amyloliquefaciens YY-27 disclosed by the invention has a certain reducing capacity on nitrite, does not grow in broth with the pH value of 9.6, grows in a brain heart infusion broth culture medium containing sodium chloride with the pH value of 6.5, does not decompose hydrogen sulfide, does not utilize glucose and lactose, does not generate urease, has a biocontrol effect on vibrio harveyi and vibrio natriuretii, has the characteristic of high yield of protease, and can be used for preparing a biological control agent for the vibrio harveyi and the vibrio natriuretii. Through YY-27 fermentation, the number of protein bands below 63KDa in the silkworm chrysalis meal is obviously increased, and the content of acid-soluble protein is obviously increased, can reach 322.63 mg / g to the maximum on the second day of fermentation, and is increased by 23.22% compared with a control group.

The application relates to the technical field of bioinformatics, and discloses a urease catalytic performance control method, device, equipment and storage medium. The method comprises the following steps: obtaining a wild-type amino acid sequence of a target urease and a three-dimensional structure corresponding to the wild-type amino acid sequence; mapping the amino acid sequence into a sequence feature unit, and mapping a local structure feature extracted from the three-dimensional structure into a structure feature unit; jointly encoding the sequence feature unit and the structure feature unit by using a decoupling multi-head cross attention module to obtain original logic values; constructing a multiple sequence alignment file based on homologous sequences, counting the amino acid frequency distribution of each residue position, and generating evolution logic values; determining an initial candidate mutant set; constructing an initial prediction model and a target prediction model; and predicting the initial candidate mutant set based on the target prediction model to obtain a target mutant with target catalytic performance. The scheme improves the control stability of urease catalytic performance.

Provided are a Saccharomyces cerevisiae strain MBP_Sc21 and the use thereof, belonging to the technical field of microorganisms. On one hand, provided is the Saccharomyces cerevisiae strain MBP_Sc21 expressing an immunonutrient β-carotene, the accession number of said strain being CGMCC No. 32663; and on the other hand, provided is the use of the Saccharomyces cerevisiae strain. The strain uses soybean meal as a solid fermentation matrix, the obtained fermented soybean meal product has a significantly increased content of crude proteins and greatly decreased contents of antinutritional factors such as oligosaccharides and urease. More importantly, the fermented soybean meal is rich in β-carotene, which endows the soybean meal protein new functional immunonutrition, thereby improving the overall nutritional value of soybean meal.

A process for removing urea from waste water of a urea synthesis plant, comprising the step of contacting the waste water with a urease enzyme and subjecting the effluent of said contacting to steam stripping to remove ammonium salts.

The invention discloses a targeted drug delivery system in a bladder. The targeted drug delivery system comprises a reagent A and a reagent B, the reagent A is a biological micromotor, platelets are taken as a natural carrier, and streptavidin is bridged and coupled with biotinylation modified urease to serve as a driving component to entrap active components; the reagent B is a urea solution. According to the invention, the natural characteristics of platelet chemotaxis tumor inflammation microenvironment are utilized to realize specific enrichment of motors in a bladder tumor area; after urea perfusion, urease catalyzes urea to generate gas power to push the motor to move, meanwhile, compact extracellular matrixes are slightly loosened, the motor is assisted to penetrate and reach the interior of a tumor and release drugs, and the delivery efficiency and targeting performance of the drugs in the bladder are remarkably improved.

The invention relates to resistant starch, a recombination method and application of the resistant starch in nephropathy treatment, the resistant starch comprises oat, brown rice, raw potatoes, black beans, red beans, mung beans and retrogradation starch, the resistant starch is recombined by adopting extrusion puffing, microwave-enzyme synergistic recombination and pressure heating-freezing and thawing cycle recombination methods, and the resistant starch can be applied to prevention and / or treatment of diabetic nephropathy. And the recombinant resistant starch is not digested in small intestines and is fermented by intestinal flora to generate SCFA (Small Form Fatty Acid) such as butyric acid and propionic acid after reaching colon, so that the proliferation of bifidobacteria and lactic acid bacteria is promoted, and urease-producing bacteria are inhibited. The resistant starch can reduce generation of intestinal uremia toxins in vivo, reduce the situation that the intestinal uremia toxins enter blood circulation through intestinal-renal axes, and relieve oxidative stress and fibrosis of renal tubules. The SCFA promotes the secretion of GLP-1, improves the insulin sensitivity and reduces the damage of hyperglycemia to the kidney.

The invention belongs to the field of biological pharmacy, and particularly relates to a helicobacter pylori vaccine recombinant protein antigen, a helicobacter pylori vaccine and a preparation method and application of the helicobacter pylori vaccine. The invention provides a novel helicobacter pylori vaccine recombinant protein antigen Sia-s aiming at the problems that antigen screening is very important and an antigen with more stable performance, higher yield and better protection effect needs to be developed when a genetic engineering vaccine is prepared. A helicobacter pylori vaccine is further prepared from the antigen, a polymer of a helicobacter pylori urease A subunit and a helicobacter pylori urease B subunit and an adjuvant dmLT. After the vaccine is immunized and inoculated through an intranasal or oropharyngeal way, an organism can be effectively stimulated to generate immune response, and an excellent immune protection effect is achieved. The antigen and the adjuvant can be obtained through escherichia coli genetic engineering expression, the source is stable and controllable, the purity is high after purification, batch and large-scale production can be achieved, and a more comprehensive prevention and control strategy is provided for helicobacter pylori infection.

The invention discloses a device and a method for synthesizing iron oxide nanoparticles by temperature-controlled chemical precipitation, and the method comprises the following steps: firstly, refrigerating and cooling a ferric salt precursor aqueous solution containing urea and a urease aqueous solution in a refrigerator at 4 DEG C to limit the activity of urease and the hydrolysis of urea; taking out and mixing the ferric salt precursor aqueous solution and the urease aqueous solution, and heating the mixed reaction solution in a water bath of 30 DEG C to form Fe (OH) 2; further, the reaction solution is heated in a water bath at 90 DEG C, Fe (OH) 2 is partially oxidized to form Fe3O4, and the Fe3O4 is heated and cured; the method is suitable for a chemical precipitation synthesis reaction triggered by an alkaline environment; the method solves the problem that the iron oxide nanoparticles synthesized by a chemical precipitation method are difficult to control through temperature; the synthesis method provided by the invention realizes segmented control of nucleation and growth of nanoparticles synthesized by a chemical precipitation method.

The present application relates to the field of veterinary in vitro diagnostic technology, in particular to a Helicobacter pylori detection reagent strip, a reagent pen and application thereof. The present application takes Helicobacter pylori antibody II as a coating antibody of a detection zone, and is used for preparing a product for detecting pet Helicobacter pylori. The product for detecting pet Helicobacter pylori detects pet Helicobacter pylori through an immunochromatography method, and the chromatography direction of a sample to be detected is vertical from bottom to top in the detection process. Therefore, the original pen appearance is improved, the present application not only provides a novel detection method for dog / cat Helicobacter pylori antigen in the animal industry, but also shortens the sample processing time, and the main advantage is that the urease content of the Helicobacter pylori positive fecal sample can be detected by one-step method when the content exceeds 5 ng / mL.

One embodiment of a blood urea nitrogen (BUN) sensor measures urea nitrogen in a biological sample by measuring a differential voltage signal based on first and second voltage signals, each representing the amount of carbon dioxide detected. The first signal represents the amount of detected carbon dioxide resulting from an enzymatic reaction in the biological sample where a urease enzyme comes into contact with the immobilized sensor surface. The second voltage signal represents the amount of background carbon dioxide detected in the biological sample. The differential voltage signal indicates the amount of urea nitrogen in the biological sample, which can be determined by appropriate correlation. Other embodiments of the BUN sensor and methods for detecting BUN are also provided, as in other embodiments.

The invention discloses anti-helicobacter pylori mouthwash as well as preparation and application thereof. The mouthwash is prepared by taking an anti-helicobacter pylori urease alpha subunit single-domain antibody and 3, 4, 5-tri-O-methylgallic acid as core active components and taking hydroxypropyl-beta-cyclodextrin, glycerol, phenoxyethanol and a citric acid-sodium citrate buffer solution as auxiliary materials. The single-domain antibody can block the catalytic activity of urease and the bacterial adhesion function; the 2, 3, 4, 5-tri-O-methyl gallic acid can penetrate through a bacterial biological membrane and destroy a cell membrane. The synergistic inhibition rate of the two components on bacterial adhesion gastric epithelial cells reaches 90.8%. Mouse experiments show that the mouthwash can reduce the bacterial load of gastric mucosa by 99.8%, relieve inflammation and repair mucosal lesion, and has no irritation to skin and oral mucosa. The mouthwash is high in stability, the retention rate of active ingredients is higher than 91% after the mouthwash is stored at 25 DEG C for 6 months, and a safe and efficient oral care product is provided for prevention and treatment of helicobacter pylori infection.

A method for bacterial contamination control of heterotrophic growth of green algae is provided. Bacterial contamination is controlled by using urea as the primary nitrogen source while simultaneously limiting the amount of nickel available to contaminating bacteria. Bacteria require nickel as a cofactor for urease enzymes in order to use urea for growth while green algae do not require nickel as a cofactor for any enzymes. Green algae use biotin as a cofactor to use urea and are biotin prototrophic. Nickel is limited by using titanium in plate heat exchangers instead of stainless steel. The preferred green algae are Chlorella sorokiniana, Chlorella vulgaris, and Auxenochlorella protothecoides.

The present invention relates to an in vitro method for enhancing the ability of urease-positive or urease-negative bacterial cells to more efficiently consume the lactose present in the environment in which they are located. The present invention further relates to bacterial cells obtained with this method, a food product and a food supplement comprising them, as well as the use thereof.

The invention relates to the technical field of medicines, in particular to a 1, 2, 4-triazole-Schiff base urease inhibitor as well as a preparation method and application thereof. The invention discloses a 1, 2, 4-triazole-Schiff base urease inhibitor for the first time, and the 1, 2, 4-triazole-Schiff base urease inhibitor has a good urease inhibition effect, can be used as a novel lead compound for helicobacter pylori resistance research, and is relatively low in toxicity to human normal cells and relatively high in safety. Meanwhile, the 1, 2, 4-triazole-Schiff base compound disclosed by the invention is simple in preparation method, relatively short in synthetic route, easy to prepare on a large scale and low in price.