68 results about "Urocaninase" patented technology

Apparatuses, systems, and methods for the performance of kidney replacement therapy having or using a dialyzer, control components, sorbent cartridge, and fluid reservoirs configured to be of a weight and size suitable to be worn or carried by an individual requiring treatment are disclosed. The system has a controlled compliance dialysis circuit, where a control pump controls the bi-directional movement of fluid across a dialysis membrane. A first sorbent cartridge is provided for use in a portable treatment module having activated carbon and zirconium oxide. The system also provides for the monitoring of an inlet and outlet conductivity of a sorbent cartridge containing urease to provide a facility to quantify or monitor the removal of urea by a detachable urea removal module.

An apparatus and method for replenishing urease in a sorbent cartridge for use in sorbent dialysis. The sorbent cartridge is configured to allow an amount of urease to be added to the sorbent cartridge. A urease solution can be injected into the sorbent cartridge to replenish the urease containing module, or solid urease can be added to the sorbent cartridge. The sorbent module can also comprise other, rechargeable, sorbent materials for removing toxins other than urea from spent dialysate.

A sorbent dialysis cartridge is provided for removal of uremic toxins from dialysate wherein the sorbent cartridges can use non-enzymatic urea-binding materials in place of urease. The cartridge can have a first sorbent layer loaded with a polymerizable urea complexing agent and a second sorbent layer loaded with a crosslinker. The crosslinker can be crosslinkable with a soluble urea complex reaction product of the polymerizable urea complexing agent and urea when passing through the first sorbent layer to form a crosslinked polymeric urea complex which is attachable to the second sorbent layer. In another option, a sorbent layer can be used which has an insolubilized crosslinked polymeric urea-bindable complex attached thereto, wherein the crosslinked polymeric urea-bindable complex can be a reaction product of a crosslinker and polymerizable urea complexing agent. Methods and sorbent dialysis systems using the cartridge, and methods of making the sorbent material, are provided.

An apparatus and method for replenishing urease in a sorbent cartridge for use in sorbent dialysis using urease pouches. The sorbent cartridge is configured to allow insertion of a urease pouch or injection of a urease solution into the sorbent cartridge containing a urease pouch. The sorbent module can also comprise other, rechargeable, sorbent materials for removing toxins other than urea from spent dialysate.

The invention discloses a recombinant helicobacter pylori protein vaccine and a preparation method thereof. The active ingredient recombinant fusion protein of the vaccine consists of recombinant LTAl-Ureal protein and LTB protein, the amino acid sequence of the recombinant LTAl-Ureal protein is shown as Seq ID No.1, the amino acid sequence of the LTB protein is shown as Seq ID No.2. Epitope-containing gene segments of Hp urease A subunit is inserted in an LTA subunit-encoded gene, a toxic part-containing segment is replaced to prepare a recombinant plasmid so as to express and obtain recombinant fusion protein polymer as a vaccine antigen, the fusion antigen and LTB protein pentamer are combined to form a hexamer structure, so that not only can the structure basis and activity of an LT mucosa adjuvant be remained, but also the toxicity can be removed, the immune response of organism muscosa can be effectively induced through immunity of mucosa path, to generate specific IgA antibody. The recombinant helicobacter pylori protein vaccine provides a vaccine manner for preventing and treating infection of helicobacter pylori.

The invention relates to preparation technique and application of non-odor soybean. The preparation technique of non-odor soybean comprises cleaning, alkali treatment, microwave treatment, drainage and drying treatment and package, wherein the aqueous alkali comprises one or mixture of a plurality of sodium carbonate, sodium bicarbonate, potassium carbonate, potassium bicarbonate, sodium hydroxide and potassium hydroxide. The preparation technique combines the chemical treatment mode with the physical treatment mode to remove the beany flavor, the activity of the lipoxidase of the treated soybean is completely lost, and bean products prepared by the non-odor soybean do not generate n-hexylaldehyde or any beany flavor; besides, the preparation technique provided by the invention can eliminate the antinutritional factors in the soybean, the activity of the hemagglutinin and the urease of the treated soybean is completely lost, and the activity loss rate of the trypsin inhibitor is larger than 75%; and meanwhile, the nutritive value of the soybean is not being damaged at all.

The present invention discloses recombinant vaccine based on the Helicobacter pylori neutral granulocyte activated protein A (NapA) antigen and its preparation process and application in inducing the protecting immune reaction resisting Helicobacter pylori infection. The vaccine consists of independent Helicobacter pylori NapA as the basic active component, or the fusion protein comprising Helicobacter pylori NapA, adhesion HpaA and urase B subunit active segment (UreB414), and one or several kinds of pharmaceutically acceptable adjuvant and excipient.

The invention discloses a type of flavanol (isobutene flavanol) compounds. The structural formulae of the compounds are shown in the specifications. The compounds have good inhibiting actions on urease, and can be used for preparing medicaments for resisting gastritis, gastric ulcer, lithangiuria and the like. The invention further discloses a preparation method of the compounds.

Solvent systems for the formulation of alkyl thiophosphoric triamide urease inhibitors, that provide stable dispersion of alkyl thiophosphoric triamides for even distribution (in low or high concentrations) onto fertilizers containing urea in liquid or solid form.

A method for or producing an organic-inorganic hybrid membrane by enzyme induction belongs to the technical field of membrane separation and comprises steps: firstly, assembling a plurality of layers of cationic polyelectrolyte and anionic polyelectrolyte alternately on the surface of a treated membrane to obtain a membrane 1; secondly assembling a plurality of layers of cationic polyelectrolyte, calcium salt and anionic polyelectrolyte and urease alternately on the surface of the membrane 1 to obtain a membrane 2; and finally soaking the membrane 2 in a urea solution, catalyzing urea in the urea solution by the urease in the membrane 2 to generate carbon dioxide, and then reacting with calcium salt on the surface of the membrane 2 to obtain calcium carbonate particles. The obtained calcium carbonate particles can effectively enhance the hydrophilic performance of the surface of the membrane, so that the membrane flux is increased.

A genistein hydroxamic acid compound has the following structural general formula. The genistein hydroxamic acid compound has good inhibitional effect on urease and can be used for preparing drugs for treating gastritis, gastric ulcer, lithangiuria and the like. A manufacturing method of the genistein hydroxamic acid compound is disclosed.

The invention discloses an artificial micro-nano robot and a preparation method thereof, and relates to the field of micro-nano robots. By taking active enzymes such as magnetic micro-nano particles,silicon dioxide microspheres, nano-gold particles, glucose oxidase, catalase and urease as materials, the artificial micro-nano robot with an asymmetric dimer structure is prepared by fusing the enzymes with the catalytic activity, the magnetic micro-nano particles, the silicon dioxide microspheres and the nano-gold particles together by utilizing a vacuum plasma sputtering technology and a chemical modification method. The method is high in repeatability and simple in preparation process. The prepared artificial micro-nano robot can rapidly move by decomposing biological media such as glucosein blood and urea in the bladder without generating any substance having toxic and side effects on the body, or by applying an exogenous magnetic field, targeted movement of the artificial micro-nanorobot in the movement direction is achieved, and the artificial micro-nano robot is applied to the biomedical fields of drug targeting delivery, tumor treatment and the like.

The invention relates to a diaryl propionyl hydroxamic compound, which has the following general formula of structure shown in the specification. The compound has relatively good inhibitory action on urease, and can be used to prepare drugs for resisting gastritis, gastric ulcer, lithangiuria and the like. The invention discloses a preparation method of the compound.

The invention discloses a 1,3,4-oxadiazole derivative containing a glucosamine fragment as well as a synthetic method of the 1,3,4-oxadiazole derivative containing the glucosamine fragment and a use of the derivative. The 1,3,4-oxadiazole derivative containing the glucosamine fragment is a novel compound, and has relatively high inhibitory activity on urease. The synthetic method disclosed by the invention is a convenient, high-yield and efficient method; paratoluensulfonyl chloride is taken as a cyclization reagent, the operation is simple and safe, the environmental pollution is small, the scope of application is wide and the yield is high.

The invention relates to a preparation method of chitosan hydrogel. The preparation method comprises the following steps: adding urea and urease into a chitosan solution, and increasing the pH value of the chitosan solution to gelate the chitosan solution, so that the chitosan hydrogel is obtained. Compared with the prior art, the method provided by the invention is simple and is easy for manufacturing, and the gelation time of the chitosan enzymatic physical-crosslinking hydrogel can be accurately regulated and controlled by adjusting concentrations of urea and urease.

The invention discloses a preparation method of a reagent for quantitatively determining a Helicobacter pylori antigen in excrement. The method comprises following steps of preparing an R2 reagent: S1, extracting Helicobacter pylori antigen (urease) protein, cloning a urease gene into a pGEX-4T-1 plasmid, fermenting and expressing by escherichia coli, and purifying by affinity chromatography to obtain a recombinant Helicobacter pylori antigen (urease); S2, preparing a polyclonal antibody of an anti-Helicobacter pylori antigen (urease); S3, preparing latex particles coated with the polyclonal antibody of the anti-Helicobacter pylori antigen (urease); and S4, dispersing in buffer solution to obtain the R2 reagent. The method is a latex enhanced immunoturbidimetry method, the prepared reagentis solution capable of directly detecting Helicobacter pylori through excrement, detection can be completed by only a excrement sample, and therefore sampling is convenient. The human body is free ofpotential safety hazards, detection efficiency is high, stability is good, specificity is high, and probability of interference is low.

The invention belongs to the field of detection, particularly relates to a rapid helicobacter pylori detection reagent, and provides the following scheme aiming at the problems of low test speed and poor test accuracy in the prior art: the detection reagent comprises sodium chloride, urea, an indicator, an ethanol solution, an absorbent, an additive and a culture medium, the absorbent comprises bischofite, plant ash and salt, the additive comprises vinegar, disodium hydrogen phosphate and sodium hydroxide, and the helicobacter pylori is rapidly cultured by adopting an improved urease method and an international common ultra-rapid culture medium culture test, so that the helicobacter pylori rapidly secretes high-activity urease to decompose a substrate urea to generate ammonia gas, and the ammonia gas is converted into the high-purity helicobacter pylori. When ammonia is dissolved in water, the PH value is increased, the color of the indicator is changed, the helicobacter pylori in saliva, tartar or gastric mucosa tissue is indirectly dyed, whether the helicobacter pylori exists or not is judged, the testing speed is high, and the accuracy is high.

This invention relates to a urase gene of streptococcus salivarius, concretely, concerning about a 57.I urase gene of streptococcus salivarius and its urase. A 57.I urase gene of streptococcus salivarius , whose nucleotide sequence is what is described in SEQ ID NO.1. Nickel ion bonding in the gene and nucleotide sequence of transit-related gene MQO are what are described in SEQ ID NO.2. The advantage of this invention is that gaining the cloning of urase gene of streptococcus salivarius U35248 by the method of segment cloning and gradually enzyme-cutting connection ,and its object gene length proceeds to 8Kb, increasing Nickel ion bonding of the gene and transit-related gene MQO compared with existing technology , therefore, expressing ureolytic activity without adding exogenous NiCl2.Attained cloning can be used for future replacement therapy in anti-caries research building without adding exogenous NiCl2,more suitable for clinical application of production alkali effect germ.

The invention relates to a method for measuring the creatinine content and a creatinine diagnosis agent box in the field of medical testing technology. The agent box comprises: buffer solution, reducing coenzyme, phosphoenolpyruvate, creatinine enzyme, atinase enzyme, urease, phosphoenolpyruvate carboxylase, malate dehydrogenase and stabilizer. It mixes the sample and the agent with a certain volume ratio to do enzyme coupling reaction; then it dispositions the end resting material on the chemical analyzer to detect the speed of the main wavelength light absorption to measure the content of the creatinine.

The invention discloses a gel reagent for rapid screening of urease activity, a kit and a rapid screening method, and belongs to the field of medical detection. The gel reagent for rapid screening of urease activity comprises 0.2-0.3 part by volume of urea; 4-8 parts by volume of phenol red; 0.02-0.1 part by volume of a proclin300; 2-10 parts by volume of a pH buffer solution; and 5-10 parts by volume of agar gel, whereinthe concentration of urea is greater than or equal to 7mg/ml, and the concentration of phenol red is greater than or equal to 30 * 10 <-3 > mg/ml. According to the detection method, a reaction product of urease and urea in saliva or a biopsy specimen is utilized to trigger corresponding index change so as to measure the urease activity. The detection steps: are as follows collecting a proper amount of samples by using an applicator device, pushing the whole samples from tweezers to a position below the surface of gel in the kit by using the applicator device, exposing the samples in the gel as many as possible, standing for 2-3 minutes, observing the color change of the gel by a visual method, determining that urease is positive if the gel becomes red, and determining that urease is negative if the color is not changed.

The invention discloses a method for capturing and detecting abnormal metabolic cells in urine. The method comprises the following steps: removing sample impurities; to be specific, taking a urine sample, pouring the urine sample into a test tube, then adding a urease aqueous solution into the test tube, carrying out standing and incubating to remove urea in the urine sample, then adding an activeadsorbent, and removing sulfur and phosphorus in the urine sample by using ultrasonic oscillation; and adding a PBS solution; to be specific, pouring the urine sample into centrifugal equipment for centrifuging, and adding the PBS solution to obtain FPBS resuspended urine cells. According to the method, the technological processes of removing sample impurities, adding a PBS solution, treating a vessel, pouring into the vessel and detecting are set and thus problems that the settling velocity difference is large caused by different viscosities and thus images are not clear and the detection result is inaccurate according to the existing cell capturing and detecting method are solved. The method for capturing and detecting abnormal metabolic cells in urine has advantages of being clear in image and accurate in detection result.

The invention relates to a stomach function and stomach cancer risk detection device and a preparation method thereof, and belongs to the field of medical detection equipment. The device is prepared by adhering a nitrocellulose membrane with a solid phase containing high-specificity gastrin 17, pepsinogen I, pepsinogen II antibody, anti-human IgG antibody, anti-human IgM antibody and goat-anti-mouse IgG polyclonal antibody, a glass fiber membrane adsorbed with colloidal gold labeled G-17, PGI and PGII antibodies and HP urease antigens, a sample pad, absorbent paper and other auxiliary materials. According to the invention, on the basis of ensuring complete release of immune colloidal gold, the reaction sensitivity is effectively improved; under the same threshold value, the dosage of immune colloidal gold can be reduced, the cost is saved, five gastric function evaluation and gastric cancer risk markers of G-17, PGI, PGII, HP urease IgG antibodies and IgM antibodies in a specimen can be detected at the same time, and the complexity of production operation is not increased. The test paper is high in sensitivity, strong in specificity, simple and convenient to operate, time-saving and strong in practicability.

N-aroyl thiosemicarbazide compounds have a structural formula as shown in the specification, have great inhibiting effects on urease and can be used for preparation of medicines for treating gastritis, gastric ulcer, lithangiuria and the like. The invention further discloses a preparation method of the N-aroyl thiosemicarbazide compounds.