71 results about "Urease enzyme" patented technology

The invention relates to a particulate composition comprising, or substantially consisting of, urea, an inhibitor, preferably a urease inhibitor and / or a nitrification inhibitor, and an additive, wherein the additive comprises components (i), (ii), and optionally (iii): (i) one or more polyhydric alcohols; preferably one or more carbohydrates, preferably selected from monosaccharides, disaccharides, and oligosaccharides; (ii) a first polymer, preferably an amine-containing polymer; more preferably a polyvinylamine or a polyalkyleneimine; and (ii) optionally, a second polymer, preferably a polar polymer; more preferably a water-soluble polar polymer. The invention further relates to a process for producing the particulate composition, an additive for a particulate composition, and the use of the particulate composition as a fertilizer.

Fertilizers with urease inhibitors and / or nitrification inhibitors are described. The fertilizer can include an extruded granule containing urea, a polymeric binder, and a nitrification inhibitor and / or an urease inhibitor.

A method for distinguishing metal ions Cu 2+ and Mn 2+ This method utilizes a pH clock reaction system of "urease-CO(NH2)2-H2SO4" as the distinguishing solution, based on the Cu of the sample to be distinguished. 2+ and Mn 2+ The reactions between Cu and the products of urea hydrolysis catalyzed by urease differ, resulting in different patterns produced by the pH clock system, thus enabling the determination of Cu... 2+ and Mn 2+ Qualitative analysis of Cu involved in this invention. 2+ and Mn 2+ The qualitative analysis method is characterized by high accuracy, ease of operation, and convenience.

The invention relates to a method for producing particles comprising urea and at least one urease inhibitor and / or at least one nitrification inhibitor, and to a suitable apparatus for carrying out the method.

The invention provides an innovative dual-mode detection method based on a dipeptide nano framework (DNPs), which is used for efficiently detecting helicobacter pylori. According to the method, firstly, a DNPs material formed by chelating dipeptide (Trp-Phe) and zinc ions (Zn (II)) is synthesized, and the material is decomposed along with the increase of the pH value in an alkaline environment, so that the Zn (II) is released; then, urea is hydrolyzed under the catalysis of urease to generate ammonia, so that the pH value of the solution is increased, and the decomposition of DNPs and the release of Zn (II) are further promoted. By utilizing an electrochemical detection mode, real-time analysis on urea and urease is realized by monitoring an electrochemical signal of Zn (II); meanwhile, in combination with the fluorescence characteristics of DNPs, the concentration of urea and urease is indirectly and quantitatively analyzed by monitoring the fluorescence intensity change. The dual-mode detection method combines the advantages of electrochemistry and fluorescence detection, has high sensitivity and real-time performance, provides an effective means for accurate detection of helicobacter pylori, and shows a wide application prospect in clinical diagnosis and sensing technologies.

The vertical shaft excavation drilling tool comprises a liquid injection mechanism used for injecting a urease solution and a drill rod, a drill bit is fixedly installed at one end of the drill rod, a groove is formed in the side wall of the drill rod and extends in the length direction of the drill rod, and the liquid injection mechanism is used for injecting the urease solution. A liquid inlet hole and a plurality of liquid outlet holes which are communicated with the groove are formed in the side wall of the drill rod, the liquid inlet hole is located at the end, away from the drill bit, of the drill rod, the liquid outlet holes are distributed in the length direction of the groove and located at the end, close to the drill bit, of the drill rod, and the liquid injection mechanism is communicated with the liquid inlet hole. The utility model aims to solve the problems that in the prior art, cement slurry materials or cement-water glass mixed slurry is mostly adopted to block cracks after water leakage of the side wall of a vertical shaft, but the cement slurry materials can shrink after being hardened, small cracks are easily generated, the cracks are gradually enlarged under hydraulic scouring, and the water blocking effect is poor. And the construction difficulty of plugging the crack after water leakage is high.

The invention discloses a urease activity detection method based on a non-reversible reaction of dopamine and resorcinol. The urease activity detection method comprises the following steps: mixing a sample to be detected, a buffer solution with the pH value of 7.0 and a urea solution with a certain concentration; adding a certain amount of dopamine and resorcinol into the mixed solution, under the condition of 25-45 DEG C, catalyzing urea hydrolysis by urease, causing the pH value of the surrounding area of the urease to rise so as to form a local alkaline environment, further inducing the reaction of the dopamine and the resorcinol to generate a blue fluorescent substance, and adding excessive hydrochloric acid to terminate the reaction after the reaction is carried out for 40-120 minutes; and detecting the fluorescence intensity of the reaction liquid by using a fluorospectro photometer, and calculating the urease activity in the sample according to a fluorescence intensity-urease concentration standard curve. The urease activity detection method based on the reaction of dopamine and resorcinol has the advantages of high detection speed, high sensitivity, good selectivity and the like.

The application discloses a water-retaining agent with salt resistance and urease inhibition, and a preparation method and application thereof, and belongs to the technical field of water-retaining in agriculture and forestry. The water-retaining agent is prepared from the following raw materials in mass parts: 100 parts of monomers, 3-10 parts of anionic natural polymer derivatives, 0.1-0.5 parts of cross-linking agents, 5-15 parts of modified lithium saponite or modified bentonite, and 0.1-1 parts of initiators; wherein the monomers are one or more of acrylic acid, acrylic acid salt or acrylamide; and the modified lithium saponite or modified bentonite is obtained by modifying lithium saponite or bentonite by quaternary ammonium salt natural polymer derivatives. The water-retaining agent has high water absorption ratio and salt water absorption ratio, can maintain stable water absorption and water retention performance in a high-salt environment, can delay the urea hydrolysis rate and reduce nitrogen loss, can realize synchronous application of the water-retaining agent and fertilizer, and can simplify farm operations.

Disclosed is a dual functional urease and nitrification inhibitor composition and method for inhibiting the hydrolysis of urea and nitrification of ammonia in soil. Methods of making the dual functional urease and nitrification inhibitor and / or a composition containing the dual functional urease and nitrification inhibitor are also disclosed.

The application discloses a kind of biological hybrid micro machine population oral delivery system of imitating Helicobacter pylori survival mechanism, belong to oral stomach medicine delivery technical field.The system is by active power module (flagellum driven chlamydomonas reinhardtii), environmental regulation module (acid urease modified on algal surface) and multifunctional load module (macrophage membrane wrapped drug-loaded nanoparticles) synergistic assembly through polydopamine (PDA) spatial conformation control layer is formed.Active power module utilizes self-movement ability to penetrate mucus barrier and extend intracavity retention time to 12 hours or more;Environmental regulation module produces local alkalization effect by catalyzing urea hydrolysis, provides chemical shield for system in extremely acid environment of pH 1.0-3.0, protects active power module from gastric acid inactivation;Multifunctional load module is accurately delivered to gastric epithelial cells by inflammation targeting effect, such as CRISPR / Cas9 biological macromolecule, and realizes up to 31.8% in-vivo gene editing efficiency.The application provides a non-invasive, modular active delivery platform for the precise treatment of gastric diseases such as gastritis through the synergistic effect of physical penetration, metabolic reprogramming and gene intervention.

The present application relates to the field of solidifying agent, and more particularly to a kind of composite solidifying agent for construction waste recycled material flow state solidified soil and preparation method thereof, comprising the following raw materials: sulphoaluminate cement 20-30 parts, slag powder 15-25 parts, recycled brick powder 10-15 parts, amino-terminated hyperbranched polymer 4-7 parts, silane coupling agent 2-4 parts, urease composite material 2-3 parts, urea microspheres 1-3 parts, calcium chloride 0.5-2 parts, construction waste recycled micro powder 10-18 parts, fly ash 5-10 parts.The solidifying agent solves the technical problems of weak interface in construction waste recycled material flow state solidified soil, enzyme activity easy deactivation, large strength fluctuation, serious shrinkage and cracking and the like through multi-component synergistic effect.

The invention provides a fertilizer synergist. The fertilizer synergist comprises a urease inhibitor, glutamic acid, organic silicon and a load carrier, the invention further provides a preparation method of the fertilizer and application of the fertilizer in improving the fertilizer utilization rate in the dry-farming potato planting process. According to the invention, the space-time synergistic effect of NBPT and L-glutamic acid discovered for the first time is taken as a basic mechanism; nBPT delays urea hydrolysis, and creates a time window for L-glutamic acid to activate root nitrogen transporter; l-glutamic acid is combined with soil particles through carboxyl of L-glutamic acid to form a slow-release microenvironment, meanwhile, root systems are promoted to secrete organic acid, and nitrogen absorption is enhanced; the organic silicon can change the soil agglomeration structure, increase the water holding capacity of the soil and reduce water infiltration and evaporation; through the synergistic effect of the three, the effective retention time of the nitrogen fertilizer in a root zone is remarkably prolonged, and the nitrogen utilization rate and the water utilization efficiency are improved.

The invention relates to an application of a ruminant non-protein nitrogen feed synergist N-methylglycine and salts thereof as a rumen urease inhibitor in ruminant feeding. The N-methylglycine and the salts thereof have an efficient inhibition effect on rumen urease, and can be used as a novel non-protein nitrogen feed synergist to be combined with urea to replace soybean meal feed to be applied to ruminant feeding.

The present invention relates to mixtures comprising a solid support comprising a urease inhibitor and another solid support comprising a nitrification inhibitor, such as 3,4-dimethylpyrazole phosphate (DMPP) or 2-(3,4-dimethyl-1H-pyrazol-1-yl)succinic acid (DMPSA), to a method for enhancing the nitrification inhibitory effect or for increasing the health of a plant using mixtures of at least one compound I and at least one compound II; to the use of mixtures comprising compounds I and II for increasing the health of a plant.

The present application belongs to the technical field of poultry and livestock breeding, and relates to a poultry and livestock breeding method for reducing ammonia emission; the method comprises the following steps: laying a three-layer composite bedding material composed of a biological activation layer, an acid-base buffer layer and a basic adsorption layer before poultry and livestock enter a house, and continuously and stably reducing ammonia emission through the synergistic effect of physical adsorption, chemical neutralization and biological degradation; daily feeding of special ammonia-reducing feed containing composite plant extract, polysaccharide enzyme preparation and urease inhibitor to reduce the excretion of nitrogen-containing substances in feces from the source; spraying of a composite ammonia-removing agent according to the ammonia concentration gradient in the house to achieve precise control; mixing of feces and bedding material after poultry and livestock defecate, and supplement of ammonia adsorbent, so that the old bedding material can be composted into organic fertilizer. The present application constructs a whole-chain ammonia emission reduction system of'source control-process blocking-end conversion', effectively realizes long-term and stable control of the ammonia concentration in the poultry house, and improves the breeding environment.

The present invention relates to a urease inhibitor composition, which can be used in agricultural and pharmaceutical sectors. The composition comprises a urease inhibitor, specifically dihydrolevoglucosenone, and can include co-solvents, plant extracts, living microorganisms or extracts thereof.

A method and a composition, characterized as having enhanced inhibitory urease activities, having a diarylheptanoid compound having a first aryl moiety group coherently bonded to a linear heptane moiety and a second aryl moiety group described by the following formula:

This invention relates to the field of biomedical technology, and provides an NH4 + Biotransformation scavenging materials and their preparation methods utilize glutamate dehydrogenase to decompose urea into NH4, which is generated by urease. + α-Ketoglutarate and nicotinamide adenine dinucleotide are converted into glutamate under the action of enzymes, thereby removing NH4, which is highly toxic to the human body. + Furthermore, MAF-7 material was synthesized by reacting zinc nitrate and 3-methyl-1,2,4-triazole in aqueous solution. Simultaneously, glutamate dehydrogenase was added during the synthesis process to immobilize the enzyme in situ onto MAF-7. This material can regenerate NH4+, a byproduct of peritoneal dialysis fluid regeneration in WAK. + It can effectively remove NH4 and also achieve long-term continuous removal. + This reduces the amount of adsorbent used and the size and weight of the device, further improving the urea treatment efficiency and solving the Na+ problem in the REDY system. + H + Risk of leakage.

The invention discloses an immobilized urease catalytic ultrafiltration membrane for degrading urea in yellow wine and a preparation method of the immobilized urease catalytic ultrafiltration membrane, and belongs to the technical field of crossing of polymer separation membranes and enzyme engineering. According to the invention, urease is stably fixed on the inner wall of the membrane hole and / or the membrane surface of the ultrafiltration membrane in a covalent bonding manner, so that the integrated catalytic separation membrane is constructed. The preparation method of the membrane comprises the following steps: performing surface amination modification on a polysulfone (PSF) matrix membrane, and further realizing covalent immobilization of urease through amidation reaction. When the prepared catalytic ultrafiltration membrane is used for treating yellow rice wine, interception of macromolecular impurities and enzymatic degradation of micromolecular urea can be synchronously completed, the urea removal rate can reach 80% or above, enzyme immobilization is firm, no dissolution risk exists, the process is simple and efficient, and a brand new solution is provided for controlling generation of ethyl carbamate (EC) in the yellow rice wine from the source.

The present application relates to the technical field of fertilizer synergism, and particularly relates to application of catechol in nitrogen synergist, aiming at the problems of unstable field application effect, complex process flow, high production cost and difficult to guarantee the effective period of existing double-effect nitrogen inhibitor, the present application first prepares the nitrogen synergist by taking catechol as the core effective component, and realizes urease and nitration double-effect inhibition by using the ortho-diphenol structure of catechol, the catechol-based nitrogen special synergistic fertilizer proposed in the present application can be prepared through three simple steps of raw material mixing, heating, water dissolving and homogenizing cooling, and efficient nitrogen double-effect inhibition can be realized by using catechol, the effective period of the catechol-based nitrogen special synergistic fertilizer is much longer than that of traditional single nitrogen inhibitor, nitrogen loss in the growth period of crops can be effectively avoided, the low addition amount feature completely solves the problem of crop seedling burning caused by high concentration of traditional inhibitor, and the normal growth of crops is guaranteed.

This invention discloses a method for producing a composite water treatment agent. It uses a poly(N-isopropylacrylamide-acrylic acid) copolymer as a temperature- and pH-responsive wall material, encapsulating a flocculant core composed of polyferric sulfate and polysilicic acid. After dopamine biomimetic modification, urease is covalently grafted onto the core, which is then combined with sodium alginate and spray-granulated. By setting response thresholds of 18-38℃ and pH 7.0-8.5, precise flocculant dosing is achieved. The method utilizes a synergistic and reversible response sequence of "response-flocculation-pH drop activating enzyme degradation-sedimentation" to correct water quality mismatches, solving problems of inaccurate dosing and residual pollution. This method is suitable for municipal wastewater and industrial cooling water treatment.

The present invention relates to a urease-inhibiting composition applicable in the agricultural and pharmaceutical sectors. The composition comprises a urease inhibitor, specifically dihydrolevoglucosenone, and may include co-solvents, plant extracts, live microorganisms, or extracts thereof.

This invention relates to solvent compositions comprising alkyl glycol ester type solvents and glycol type solvents, non-aqueous solvent delivery formulations comprising solvent compositions and urease inhibitors dissolved in solvent compositions, fertilizers comprising urea-based components and non-aqueous solvent delivery formulations, and the use of fertilizers in agricultural applications.

The utility model provides a urea production system with a triple-effect function. The urea production system comprises a urea solution conveying pipeline, a first-section evaporator, a second-section evaporator, a granulation tower, a conveying device, a mixing roller and a stock bin which are connected in sequence, the urea solution conveying pipeline is further connected with a humic acid solution dosing tank and a furfural acid solution dosing tank, a mixed inhibitor dosing device is arranged above the conveying device, and the mixed inhibitor dosing device is connected with a urease inhibitor dosing device and a denitrification inhibitor dosing device; urea produced by the urea production system has three functions of loosening soil, promoting rapid growth of root systems and inhibiting rapid loss of fertilizer efficiency.

The application discloses a Cu II Coordination polymer, preparation method thereof and application of the coordination polymer as urease inhibitor. The technical scheme is as follows: 1,4-butanediamine is used as raw material to synthesize N,N'-bis(3-pyridine carboxamide)-1,4-butane, then the N,N'-bis(3-pyridine carboxamide)-1,4-butane is used as a ligand to construct a novel Cu II Coordination polymer by using a hydrothermal method, the coordination polymer has the characteristics of stable structure, simple process and good repeatability, the coordination environment of the Cu II Coordination polymer is analyzed and urease inhibition experiment is carried out, the coordination polymer has good inhibition effect on urease activity, can be added into a fertilizer as a novel urease inhibitor, inhibits urease activity, delays urea hydrolysis time, and makes urea hydrolysis blocked, so that soil urea ammonia volatilization is reduced, nitrogen loss is reduced and utilization rate is improved by applying the substance.

Disclosed are methods of extracting an enzyme, comprising soaking a seed in an aqueous solution, homogenizing the seed to produce a homogenized suspension, and filtering the homogenized suspension to produce a crude extract, wherein the crude extract comprises urease and non-urease proteins.

The application relates to a preparation method of a copper-based coordination polymer urease inhibitor suitable for fertilizers, and relates to a preparation method of agricultural fertilizers. In the application, N,N'-bis(3-pyridine carboxamide)-1,2-ethane and benzoic acid are used as ligands to construct a copper-based coordination polymer. Through urease inhibition experiments, the copper-based coordination polymer is added to the fertilizer as a urease inhibitor, can be well filled in the active pocket of urease, interacts in the active binding pocket of urease, occupies the binding site belonging to nickel, causes irreversible inhibition of urease activity, slows down the urea hydrolysis speed, delays the conversion of urea state nitrogen into ammonium state nitrogen in the soil fertilization process, achieves the effect of inhibiting urease activity, reduces the additional loss of nitrogen fertilizer, and improves the nitrogen fertilizer utilization rate. When applied to the fertilizer, the application has the characteristics of safety, effectiveness and environmental pollution reduction.

The invention discloses a method for detecting urea in urine based on pH sensitive carbon dots and urease, and belongs to the technical field of analysis and detection. According to the method for detecting the urea in the urine based on the pH-sensitive carbon dots and the urease, the urea in the urine is detected on the basis that the pH-sensitive carbon is synthesized through a hydrothermal method by taking isoprenaline hydrochloride as a precursor, the detection sensitivity is high, and the specificity and the anti-interference performance are high; the method is simple, can realize high-efficiency detection of urea in a complex liquid environment of urine, is simple, has strong operability, and has wide application prospects in the field of medical detection.