104 results about "Urease enzyme" patented technology

The invention relates to a particulate composition comprising, or substantially consisting of, urea, an inhibitor, preferably a urease inhibitor and / or a nitrification inhibitor, and an additive, wherein the additive comprises components (i), (ii), and optionally (iii): (i) one or more polyhydric alcohols; preferably one or more carbohydrates, preferably selected from monosaccharides, disaccharides, and oligosaccharides; (ii) a first polymer, preferably an amine-containing polymer; more preferably a polyvinylamine or a polyalkyleneimine; and (ii) optionally, a second polymer, preferably a polar polymer; more preferably a water-soluble polar polymer. The invention further relates to a process for producing the particulate composition, an additive for a particulate composition, and the use of the particulate composition as a fertilizer.

The invention discloses a probiotic animal feed and a preparation method thereof, and belongs to the technical field of animal feeds, the probiotic animal feed comprises the following components by mass: 40-55 parts of caragana microphylla powder, 20-30 parts of fermented soybean meal, 3-5 parts of a composite probiotic preparation, 5-10 parts of a medicinal and edible additive, and 2-3 parts of a mineral premix. According to the composite probiotic preparation, bacillus licheniformis, pediococcus pentosaceus and saccharomyces cerevisiae boulardii synergistically improve the fiber decomposition efficiency, inhibit pathogenic bacteria and reduce ammonia nitrogen emission. The medicinal and edible additive is of a multi-layer micro-capsule structure, the outer layer is carboxymethyl chitosan and copper gluconate modified nano zeolite, active substances in the middle layer are rapidly released through rumen pH response, and the middle layer contains a yucca extract, a liquorice extract and other components and can inhibit urease activity and ammonia-producing bacteria; in the inner layer, a nano-selenium-vitamin E compound, curcumin and the like are slowly released through sodium alginate-pectin gel, so that intestinal metabolism is optimized. The process of pretreating caragana microphylla through steam explosion and performing solid-state fermentation on soybean meal through aspergillus oryzae is utilized, so that grain-free production is realized.

The invention discloses a coated defluorination composite material based on a microbial mineralization technology and a preparation method thereof. According to the coated defluorination composite material, urease vaterite calcium carbonate particles are used as a material core part, and the outer surface of the core part is coated with a calcium cross-linked porous composite film to serve as a coating layer of the material; the calcium cross-linked porous composite membrane is a porous membrane material formed by cross-linking calcium ions and a porous matrix, and the porous matrix is selected from any one of chitosan, cellulose, sodium alginate and porous silicon; the urease vaterite calcium carbonate particles are generated in situ after urea hydrolysis is catalyzed by urease and a calcium source is added. The composite material has the advantages of efficient defluorination effect and good stability when used in fluorine-containing wastewater, and the preparation method of the coated defluorination composite material is simple and easy to implement.

The present invention relates to urease inhibitor compositions containing tert-butylhydroquinone and optionally an additive component to enhance the inhibitory properties of tert-butylhydroquinone. Further described are uses of these compositions in agriculture to increase nutrient uptake and inhibit urease enzyme activity.

Fertilizers with urease inhibitors and / or nitrification inhibitors are described. The fertilizer can include an extruded granule containing urea, a polymeric binder, and a nitrification inhibitor and / or an urease inhibitor.

A method for distinguishing metal ions Cu 2+ and Mn 2+ This method utilizes a pH clock reaction system of "urease-CO(NH2)2-H2SO4" as the distinguishing solution, based on the Cu of the sample to be distinguished. 2+ and Mn 2+ The reactions between Cu and the products of urea hydrolysis catalyzed by urease differ, resulting in different patterns produced by the pH clock system, thus enabling the determination of Cu... 2+ and Mn 2+ Qualitative analysis of Cu involved in this invention. 2+ and Mn 2+ The qualitative analysis method is characterized by high accuracy, ease of operation, and convenience.

The invention relates to a method for producing particles comprising urea and at least one urease inhibitor and / or at least one nitrification inhibitor, and to a suitable apparatus for carrying out the method.

A method for contamination control when growing yeasts is provided. Bacterial contamination is controlled by using urea as the primary nitrogen source while simultaneously limiting the amount of nickel available to contaminating bacteria. Bacteria require nickel as a cofactor for urease enzymes in order to use urea for growth while yeasts do not require nickel as a cofactor for any enzymes. Nickel is limited by using metals in heat exchangers that do not leach nickel. Ethyl carbamate is limited by using a carbon / nitrogen ratio that consumes all urea during fermentation. After fermentation completes, yeast is recycled using centrifugation, enabling use of high concentrations of yeast to reduce fermentation time from 48 hours to 12 hours and to eliminate bacterial contamination from growth on free amino nitrogen.

A method for stabilizing a reducing slag includes the steps of: subjecting a reducing slag which contains an alkaline earth metal oxide to a drying treatment, so as to obtain a dried reducing slag; mixing a urease-producing bacterium, a fermentation medium, and a nutrient, followed by conducting a fermentation treatment, so as to obtain a fermentation product containing urease; subjecting the fermentation product to a foam fractionation treatment, so as to obtain a foam containing urease; mixing the dried reducing slag, the foam containing urease, and urea, followed by conducting a urea hydrolysis reaction and a precipitation reaction in sequence, so as to obtain a product containing a stabilized reducing slag and a residual liquid; and subjecting the product to a solid-liquid separation treatment, so as to separate the stabilized reducing slag and the residual liquid from the product.

The invention provides an innovative dual-mode detection method based on a dipeptide nano framework (DNPs), which is used for efficiently detecting helicobacter pylori. According to the method, firstly, a DNPs material formed by chelating dipeptide (Trp-Phe) and zinc ions (Zn (II)) is synthesized, and the material is decomposed along with the increase of the pH value in an alkaline environment, so that the Zn (II) is released; then, urea is hydrolyzed under the catalysis of urease to generate ammonia, so that the pH value of the solution is increased, and the decomposition of DNPs and the release of Zn (II) are further promoted. By utilizing an electrochemical detection mode, real-time analysis on urea and urease is realized by monitoring an electrochemical signal of Zn (II); meanwhile, in combination with the fluorescence characteristics of DNPs, the concentration of urea and urease is indirectly and quantitatively analyzed by monitoring the fluorescence intensity change. The dual-mode detection method combines the advantages of electrochemistry and fluorescence detection, has high sensitivity and real-time performance, provides an effective means for accurate detection of helicobacter pylori, and shows a wide application prospect in clinical diagnosis and sensing technologies.

The vertical shaft excavation drilling tool comprises a liquid injection mechanism used for injecting a urease solution and a drill rod, a drill bit is fixedly installed at one end of the drill rod, a groove is formed in the side wall of the drill rod and extends in the length direction of the drill rod, and the liquid injection mechanism is used for injecting the urease solution. A liquid inlet hole and a plurality of liquid outlet holes which are communicated with the groove are formed in the side wall of the drill rod, the liquid inlet hole is located at the end, away from the drill bit, of the drill rod, the liquid outlet holes are distributed in the length direction of the groove and located at the end, close to the drill bit, of the drill rod, and the liquid injection mechanism is communicated with the liquid inlet hole. The utility model aims to solve the problems that in the prior art, cement slurry materials or cement-water glass mixed slurry is mostly adopted to block cracks after water leakage of the side wall of a vertical shaft, but the cement slurry materials can shrink after being hardened, small cracks are easily generated, the cracks are gradually enlarged under hydraulic scouring, and the water blocking effect is poor. And the construction difficulty of plugging the crack after water leakage is high.

The invention discloses a urease activity detection method based on a non-reversible reaction of dopamine and resorcinol. The urease activity detection method comprises the following steps: mixing a sample to be detected, a buffer solution with the pH value of 7.0 and a urea solution with a certain concentration; adding a certain amount of dopamine and resorcinol into the mixed solution, under the condition of 25-45 DEG C, catalyzing urea hydrolysis by urease, causing the pH value of the surrounding area of the urease to rise so as to form a local alkaline environment, further inducing the reaction of the dopamine and the resorcinol to generate a blue fluorescent substance, and adding excessive hydrochloric acid to terminate the reaction after the reaction is carried out for 40-120 minutes; and detecting the fluorescence intensity of the reaction liquid by using a fluorospectro photometer, and calculating the urease activity in the sample according to a fluorescence intensity-urease concentration standard curve. The urease activity detection method based on the reaction of dopamine and resorcinol has the advantages of high detection speed, high sensitivity, good selectivity and the like.

The application discloses a water-retaining agent with salt resistance and urease inhibition, and a preparation method and application thereof, and belongs to the technical field of water-retaining in agriculture and forestry. The water-retaining agent is prepared from the following raw materials in mass parts: 100 parts of monomers, 3-10 parts of anionic natural polymer derivatives, 0.1-0.5 parts of cross-linking agents, 5-15 parts of modified lithium saponite or modified bentonite, and 0.1-1 parts of initiators; wherein the monomers are one or more of acrylic acid, acrylic acid salt or acrylamide; and the modified lithium saponite or modified bentonite is obtained by modifying lithium saponite or bentonite by quaternary ammonium salt natural polymer derivatives. The water-retaining agent has high water absorption ratio and salt water absorption ratio, can maintain stable water absorption and water retention performance in a high-salt environment, can delay the urea hydrolysis rate and reduce nitrogen loss, can realize synchronous application of the water-retaining agent and fertilizer, and can simplify farm operations.

Disclosed is a dual functional urease and nitrification inhibitor composition and method for inhibiting the hydrolysis of urea and nitrification of ammonia in soil. Methods of making the dual functional urease and nitrification inhibitor and / or a composition containing the dual functional urease and nitrification inhibitor are also disclosed.

Preventing the flow of soil grains into oil and gas wells is highly desirable for the protection of hydrocarbon production. Geotechnical engineering solutions addressing this issue may utilize enzyme-induced precipitation methods to consolidate and increase shear strength. Methods may comprise: providing a mixture comprising urea, a urease enzyme, calcium chloride, magnesium chloride, and a cellulose; contacting at least a portion of the mixture with at least a portion of a subterranean formation; and forming a biomineral precipitate within the subterranean formation. The biomineral precipitate, comprised of a carbonate mineral and an evaporite mineral, may consolidate particles within the subterranean formation to form a consolidated body.

The application discloses a kind of biological hybrid micro machine population oral delivery system of imitating Helicobacter pylori survival mechanism, belong to oral stomach medicine delivery technical field.The system is by active power module (flagellum driven chlamydomonas reinhardtii), environmental regulation module (acid urease modified on algal surface) and multifunctional load module (macrophage membrane wrapped drug-loaded nanoparticles) synergistic assembly through polydopamine (PDA) spatial conformation control layer is formed.Active power module utilizes self-movement ability to penetrate mucus barrier and extend intracavity retention time to 12 hours or more;Environmental regulation module produces local alkalization effect by catalyzing urea hydrolysis, provides chemical shield for system in extremely acid environment of pH 1.0-3.0, protects active power module from gastric acid inactivation;Multifunctional load module is accurately delivered to gastric epithelial cells by inflammation targeting effect, such as CRISPR / Cas9 biological macromolecule, and realizes up to 31.8% in-vivo gene editing efficiency.The application provides a non-invasive, modular active delivery platform for the precise treatment of gastric diseases such as gastritis through the synergistic effect of physical penetration, metabolic reprogramming and gene intervention.

The present application relates to the field of solidifying agent, and more particularly to a kind of composite solidifying agent for construction waste recycled material flow state solidified soil and preparation method thereof, comprising the following raw materials: sulphoaluminate cement 20-30 parts, slag powder 15-25 parts, recycled brick powder 10-15 parts, amino-terminated hyperbranched polymer 4-7 parts, silane coupling agent 2-4 parts, urease composite material 2-3 parts, urea microspheres 1-3 parts, calcium chloride 0.5-2 parts, construction waste recycled micro powder 10-18 parts, fly ash 5-10 parts.The solidifying agent solves the technical problems of weak interface in construction waste recycled material flow state solidified soil, enzyme activity easy deactivation, large strength fluctuation, serious shrinkage and cracking and the like through multi-component synergistic effect.

The invention provides a fertilizer synergist. The fertilizer synergist comprises a urease inhibitor, glutamic acid, organic silicon and a load carrier, the invention further provides a preparation method of the fertilizer and application of the fertilizer in improving the fertilizer utilization rate in the dry-farming potato planting process. According to the invention, the space-time synergistic effect of NBPT and L-glutamic acid discovered for the first time is taken as a basic mechanism; nBPT delays urea hydrolysis, and creates a time window for L-glutamic acid to activate root nitrogen transporter; l-glutamic acid is combined with soil particles through carboxyl of L-glutamic acid to form a slow-release microenvironment, meanwhile, root systems are promoted to secrete organic acid, and nitrogen absorption is enhanced; the organic silicon can change the soil agglomeration structure, increase the water holding capacity of the soil and reduce water infiltration and evaporation; through the synergistic effect of the three, the effective retention time of the nitrogen fertilizer in a root zone is remarkably prolonged, and the nitrogen utilization rate and the water utilization efficiency are improved.

The invention relates to a self-repairing anti-scaling polyurea-cellulose nanocrystalline coating as well as a preparation method and application thereof, and belongs to the technical field of chemistry and chemical engineering. The coating comprises an aqueous polyurea-disulfide bond dynamic matrix, cellulose nanocrystals, a urease / urea microcapsule self-repairing agent and an auxiliary agent, and the urease / urea microcapsule self-repairing agent comprises urease, urea, first isocyanate, polyether amine and an emulsifier. The main structure of the coating is a dynamic polyurea network containing disulfide bonds, and the network is filled with nanoscale coarse structure cellulose nanocrystals and a urease / urea microcapsule self-repairing agent. Wherein the disulfide bond dynamic polyurea network structure improves the structural stability and durability of the coating in high-temperature, high-speed sand-containing washing and strong acid / weak acid environments, and the urease / urea microcapsule self-repairing agent endows the coating with the capability of quickly repairing damage in a high-humidity environment.

The invention relates to an application of a ruminant non-protein nitrogen feed synergist N-methylglycine and salts thereof as a rumen urease inhibitor in ruminant feeding. The N-methylglycine and the salts thereof have an efficient inhibition effect on rumen urease, and can be used as a novel non-protein nitrogen feed synergist to be combined with urea to replace soybean meal feed to be applied to ruminant feeding.

The present invention relates to mixtures comprising a solid support comprising a urease inhibitor and another solid support comprising a nitrification inhibitor, such as 3,4-dimethylpyrazole phosphate (DMPP) or 2-(3,4-dimethyl-1H-pyrazol-1-yl)succinic acid (DMPSA), to a method for enhancing the nitrification inhibitory effect or for increasing the health of a plant using mixtures of at least one compound I and at least one compound II; to the use of mixtures comprising compounds I and II for increasing the health of a plant.

The invention relates to the technical field of compound fertilizers, and discloses a nitrogen-phosphorus-potassium balanced compound fertilizer containing an alginic acid chelating carrier, which comprises the following raw materials by weight: urea, monocalcium phosphate, potassium sulfate compounded potassium chloride, alginic acid, medium trace elements, a slow release regulator, a bioactive substance, diatomite, gamma-polyglutamic acid and a coating material. According to the nitrogen-phosphorus-potassium balanced compound fertilizer containing the alginic acid chelating carrier and the preparation method of the nitrogen-phosphorus-potassium balanced compound fertilizer, the alginic acid microporous gel carrier prepared by gamma-ray irradiation degradation and electrostatic spinning technologies has an ultra-large specific surface area and a nanoscale microporous structure, and can efficiently adsorb basic nutrients such as nitrogen, phosphorus and potassium and medium trace elements to form a stable chelating system; by combining a slow-release regulator composed of carboxymethyl chitosan and humic acid and a multilayer coating design of a urease inhibitor, a nitrification inhibitor, PLA microcapsules and fatty acid calcium, nutrient loss is reduced, and the utilization rate of the fertilizer is remarkably increased.

The present invention discloses a pomalidomide-linked urea feed additive and its preparation method and application, belonging to the technical field of functional feed additive synthesis. The technical solution of the present invention is as follows: the pomalidomide-linked urea feed additive molecule has a structure of 2-Boc aminobenzoic acid as the starting material, first reacting to obtain 2-(2,6-dioxopiperidin-3-yl)-4-aminoisoindoline-1,3-dione, and then converting the amine group on the structure into azide, and obtaining the target compound through a click reaction. The compound is loaded with exosomes by a supercritical method, has a certain inhibitory effect on cattle urease, and can be used as a potential feed additive.

Preventing the flow of soil grains into oil and gas wells is highly desirable for the protection of hydrocarbon production. Geotechnical engineering solutions addressing this issue may utilize enzyme-induced precipitation methods to consolidate and increase shear strength. Methods may comprise: providing a mixture comprising urea, a urease enzyme, calcium chloride, magnesium chloride, and a cellulose; contacting at least a portion of the mixture with at least a portion of a subterranean formation; and forming a biomineral precipitate within the subterranean formation. The biomineral precipitate, comprised of a carbonate mineral and an evaporite mineral, may consolidate particles within the subterranean formation to form a consolidated body.

The present application belongs to the technical field of poultry and livestock breeding, and relates to a poultry and livestock breeding method for reducing ammonia emission; the method comprises the following steps: laying a three-layer composite bedding material composed of a biological activation layer, an acid-base buffer layer and a basic adsorption layer before poultry and livestock enter a house, and continuously and stably reducing ammonia emission through the synergistic effect of physical adsorption, chemical neutralization and biological degradation; daily feeding of special ammonia-reducing feed containing composite plant extract, polysaccharide enzyme preparation and urease inhibitor to reduce the excretion of nitrogen-containing substances in feces from the source; spraying of a composite ammonia-removing agent according to the ammonia concentration gradient in the house to achieve precise control; mixing of feces and bedding material after poultry and livestock defecate, and supplement of ammonia adsorbent, so that the old bedding material can be composted into organic fertilizer. The present application constructs a whole-chain ammonia emission reduction system of'source control-process blocking-end conversion', effectively realizes long-term and stable control of the ammonia concentration in the poultry house, and improves the breeding environment.

The present invention relates to a urease inhibitor composition, which can be used in agricultural and pharmaceutical sectors. The composition comprises a urease inhibitor, specifically dihydrolevoglucosenone, and can include co-solvents, plant extracts, living microorganisms or extracts thereof.

The present application relates to the application of trimethylglycine and its salts as urease inhibitors in nitrogen fertilizer synergism. Trimethylglycine and its salts have high inhibitory effect on urease, and can be applied as a new type of nitrogen fertilizer synergist in crop planting.

The present invention relates to a fertilizer additive, and in particular to a liquid preparation containing a urease inhibitor. The liquid preparation comprises the following components in parts by weight: 16-36 parts of a urease inhibitor, 41-150 parts of a solvent, 3-14 parts of a cosolvent, and 0.6-3 parts of a regulator; wherein the urease inhibitor is N-n-butylthiophosphoric triamide; the solvent is a mixture of one or more ketones having 3 to 7 carbon atoms; the cosolvent is a mixture of one or more diol or triol derivatives; and the regulator is a mixture of one or more triethanolamine, diethanolamine, or an alkyldiethanolamine having an alkyl group having 1 to 4 carbon atoms. The liquid preparation containing a urease inhibitor prepared using the liquid preparation has good solubility and anti-coagulation properties at room temperature and low temperature, and can be stably present at both room temperature and high temperature. When applied to fertilizer, the fertilizer does not dissolve and does not clump.

A method and a composition, characterized as having enhanced inhibitory urease activities, having a diarylheptanoid compound having a first aryl moiety group coherently bonded to a linear heptane moiety and a second aryl moiety group described by the following formula: