213 results about "Urease enzyme" patented technology

The present invention is directed to degassing devices for dialysate circuits. One embodiment has a first housing and a second housing positioned within the first housing in an annular relationship. A second embodiment comprises a dialysate regeneration system with urease, a dialyzer, and a housing with an external wall, where the external wall is exposed to atmosphere and comprises a material that passes gas but does not pass liquid and where the housing is positioned between the urease and dialyzer.

An odor-inhibiting fiber having a cellulosic fiber and an odor-inhibiting formulation. The odor-inhibiting formulation may contain an odor-inhibiting agent, such as a biocide, an enzyme, a urease inhibitor. The odor-inhibiting formulation also may contain a liquid carrier such as a hydrophobic or hydrophilic organic liquid, or a mixture of a hydrophobic and hydrophilic organic liquid. The cellulosic fiber is impregnated with the odor-inhibiting formulation to produce fiber having odor-inhibiting characteristics. The resultant odor-inhibiting fiber is useful in making absorbent articles with odor-inhibiting characteristics. The fiber of the embodiments prevents odor by inhibiting bacteria growth and ammonia production, especially when used in an absorbent article such as a diaper or adult incontinence device.

A sorbent hemodialysis system includes a dialyzer configured to receive a flow of clean dialysate from a reservoir and to output an unclean dialysate flow. The system also includes a sorbent component having a urease section and a sorbent section through which the unclean dialysate flow from the dialyzer passes, wherein the sorbent component removes urea from the dialysate. The system further comprises a membrane electrolyzer that receives at least a portion of said clean dialysate flow and separates the dialysate flow into an acidic component flow and a base component flow. A mixing conduit combines the base component flow from the membrane electrolyzer and an output dialysate solution from the urease section of the sorbent component to separate the dialysate solution into an ammonia gas amount and ammonia liquid amount. A gas vent is used to vent the ammonia gas amount, and the sorbent section with a suitable amount of zirconium phosphate (ZrP) removes the ammonia liquid amount from the unclean dialysate flow before flowing the clean dialysate to the reservoir. The system can further include a second mixing conduit upstream of the sorbent section of the sorbent component, the second mixing conduit combining the acidic component flow and the ammonia liquid amount in the dialysate solution to increase the pH of the dialysate solution to about 7.5 prior to returning to the reservoir.

Solutions are prepared by dissolving N-(n-butyl)-thiophosphoric triamide (NBPT) in one or more N-substituted morpholines and mixtures thereof. The solutions may be used in urea fertilizers to reduce nitrogen volatilization, or to reduce the odor of animal waste or urine. Methods of preparing urea fertilizers and the resultant products are also described.

The present invention provides improved solvent systems for the preparation of liquid formulations of urease inhibitors, specifically NBPT, comprising aryl alkyl alcohols. The solvent systems provided good solubility of the urease inhibitor as well as at least one of improved stability, lower flammability, lower toxicity, improved cold temperature storage, improved handling, improved adsorption onto and / or solubility with solid media such as urea. Methods of making and using the compositions are also provided.

An apparatus and method for replenishing urease in a sorbent cartridge for use in sorbent dialysis. The sorbent cartridge is configured to allow an amount of urease to be added to the sorbent cartridge. A urease solution can be injected into the sorbent cartridge to replenish the urease containing module, or solid urease can be added to the sorbent cartridge. The sorbent module can also comprise other, rechargeable, sorbent materials for removing toxins other than urea from spent dialysate.

An apparatus and method for replenishing urease in a sorbent cartridge for use in sorbent dialysis using urease pouches. The sorbent cartridge is configured to allow insertion of a urease pouch or injection of a urease solution into the sorbent cartridge containing a urease pouch. The sorbent module can also comprise other, rechargeable, sorbent materials for removing toxins other than urea from spent dialysate.

An apparatus and method for replenishing urease in a sorbent cartridge for use in sorbent dialysis. The system is configured to allow insertion of a urease pouch, injection of a urease solution, or addition of a urease cartridge, into a dialysis cabinet containing a dialysis flow loop. The urease can be dissolved and the resulting urease solution added to the sorbent cartridge in the flow loop to replenish the urease within the sorbent cartridge. The sorbent cartridge can also comprise other, rechargeable, sorbent materials for removing toxins other than urea from spent dialysate.

The invention discloses a long-acting synergistic controlled release fertilizer, which mainly comprises a nitrogen fertilizer, a phosphate fertilizer, a potash fertilizer, a biological inoculant, a urease inhibitor, a nitrification inhibitor and poly(aspartic acid). Specifically, in terms of weight percentage, the nitrogen fertilizer accounts for 10-60, the phosphate fertilizer accounts for 10-60, the potash fertilizer accounts for 10-60, an organic biological multielement compound agent accounts for 5-50, wherein the biological inoculant accounts for 6-8, a liquid binder accounts for 3-5, a filling material accounts for 10-20, livestock and poultry manure or humic acid accounts for 50-80, the urease inhibitor accounts for 0.1-0.15, the nitrification inhibitor accounts for 0.15-0.2, and the poly(aspartic acid) accounts for 6-20. The invention aims to overcome the defects in the prior art, ordinary fertilizers have the disadvantages of short effective period, unreasonable nutrient proportion, unscientific and unbalanced nutrient supply, especially the nitrogen fertilizer conflict is more prominent. The long-acting synergistic controlled release fertilizer is released along with the absorption rate of crops, fertilizer loss can be effectively reduced, and saving, yield increase, synergia, convenience, environmental protection and multiple objectives can be achieved, and agricultural sustainable development can be realized. The long-acting synergistic controlled release fertilizer is simple and feasible in application, and is suitable for China's actual conditions, thus being an inevitable course for the world fertilizer development in the 21st century.

The invention discloses a melt granulated polypeptide stable fertilizer and a preparation method thereof, relating to the field of compound fertilizers. The preparation method comprises the following steps: proportionally preparing urea, monoammonium phosphate, ammonium chloride, potassium chloride, compound urease inhibitor, compound nitrification inhibitor, polypeptide synergist and talcum powder; and heating the raw materials, mixing, carrying out spray granulation, cooling, screening, measuring and packaging to obtain the finished product. The stable fertilizer disclosed by the invention has the advantages of high utilization ratio, no pollution and obvious yield increase effect, promotes absorption of microelements, improves the quality of the crops, and enhances the stress tolerance.

The invention discloses a melt granulation polypeptide stability lost control compound fertilizer and a preparing method thereof, which relate to the technical field of compound fertilizers. The melt granulation polypeptide stability lost control compound fertilizer adopts urea, monoammonium phosphate, ammonium chloride, potassium chloride, zinc oxide, ferrous oxide, manganese sulfate, combined urease inhibitors, combined nitrification inhibitors, polypeptide synergist and fillings as raw matreials, and all the raw materials are dosed according to a specified ratio. The polypeptide stability lost control compound fertilizer product is obtained through processes such as mixing stirring, spray granulating, cooling curing, sieving, measuring and packing. The melt granulation polypeptide stability lost control compound fertilizer product has the advantages of improving fertilizer use ratio, being completely degraded without residuals and being high in absorbed efficiency.

An inhibitor composition contains dicyandiamide as a nitrification inhibitor, alkyl thiophosphoric triamide as a urease inhibitor, or a combination thereof, dissolved in a liquid medium comprising an organic solvent selected from, among others, one or more polar aprotic solvents, including one or more organophosphates, amine solvents, heterocyclic alcohol solvents, and mixtures thereof, is useful in making fertilizer compositions and in a method of fertilizing target plants.

The invention provides a special slow control / release fertilizer for an eggplant. The fertilizer is characterized by comprising, in terms of mass percent, 0.05% to 0.08% of n-butyl triamine thiophosphate, 0.05 to 0.08% of hydroquinone and 0.24 to 0.28% of dicyandiamide. According to the invention, the special slow control / release fertilizer is prepared by combining macro-element, medium-element and trace-element fertilizers like nitrogen, phosphorus, potassium, calcium, magnesium, molybdenum, boron, zinc and iron with organic fertilizers like high-quality decomposed rapeseed dregs, chicken manure and grass carbon, adding a urease inhibitor and a nitrification inhibitor and carrying out mixing; the special slow control / release fertilizer has a slow release effect in soil, is rich in a variety of nutrient elements needed in growth of the eggplant, can meet demands of the eggplant for nutrients in different growth and development phases for a long time and effectively improves output of the eggplant.

The present invention provides an improved method of producing a stable acid denatured soy / urea adhesive having improved wet and dry strengths, with more efficient production and lower production costs. The method comprises combining urea with soy flour that has been acid treated until denatured and substantially free from urease activity. The soy flour is preferably lowered to a pH of 2.0 to 4.0 for at least 1 minute. Optionally, the method may also include adding a crosslinking agent, diluent or both to the soy flour / urea adhesive and / or adding an emulsified or dispersed polymer. Adhesives and dispersions prepared according to the methods of this invention offer increased stability and strength properties.

The invention provides a microcological feed additive for lowering the concentration of ammonia in a livestock and poultry house and application of the microcological feed additive. The microcological feed additive comprises the following components: bacillus subtilis in the ratio of 3.92-4.08*10<9>pcs / g, bacillus licheniformis in the ratio of 0.98-1.02*10<9>pcs / g, butyrate clostridium in the ratio of 1.96-2.04*10<8>pcs / g, SSF (sodium fluosilicate) enzyme prepared by aspergillus oryzae in the ratio of 148-152mg / g and a carrier in the ratio of 98-102mg / g. The microcological feed additive for lowering the concentration of ammonia in the livestock and poultry house can improve protein absorbing capacity of an intestinal canal of livestock and poultry through producing enzyme, thus a nitrogensource is reduced; growth of putrefying bacteria is inhibited by beneficial bacteria, thus emission of the ammonia is reduced; and the activity of urease is inhibited, and the ammonia generated by uric acid and urea under the catalysis of urease is reduced, thus the aim of lowering the concentration of the ammonia in a livestock house is achieved.

The invention provides a special slow / controlled release fertilizer for capsicum. The special slow / controlled release fertilizer for capsicum is characterized in that the special slow / controlled release fertilizer for capsicum comprises, by mass, 0.10-0.15% of N-(n-Butyl)thiophosphoric triamide or quinhydrone and 0.25-0.30% of dicyandiamide. A preparation method of the special slow / controlled release fertilizer for capsicum comprises the following steps: combining fertilizers of macro, medium and trace elements of N, P, K, Mg, B, Zn and the like with organic fertilizers comprising high-quality decomposed rapeseed meal, chicken manure and the like, adding a urease inhibitor and a nitration inhibitor, and mixing. The special slow / controlled release fertilizer for capsicum has a slow release effect in soil, is rich in all nutritional elements specially required by the growth of capsicum, can effectively satisfy the nutrient needs of the capsicum of different growth stages, and can effectively improve the capsicum output.

The invention discloses a pickle prolonged-effect-time compound fertilizer and a preparation method thereof. The pickle prolonged-effect-time compound fertilizer includes nitrogen fertilizer, phosphate fertilizer, potash fertilizer, urease inhibitor, nitrification inhibitor and fertilizer synergist, wherein the total mass fraction of the nitrogen fertilizer, the phosphate fertilizer and the potash fertilizer is not lower than 96%; the preparation method of the pickle prolonged-effect-time compound fertilizer includes the steps as follows: smashing urea to powder so as to btain urea powder; adding water for fully dissolving borax, thiourea, dicyandiamide and polyaspartic acid to obtain a mixed solution; spraying all the mixed solution into the urea powder and then mixing with calcium superphosphate and potassium chloride to obtain a mixture; adding a binder; and granulating the mixture and then drying so as to obtain products. The pickle prolonged-effect-time compound fertilizer has high utilization rate, long fertilizer effect time, using safety and lower relative cost, can meet primary fertilizer application of the pickle, and can obviously reduce the convent of nitrate in the pickle.

The invention discloses a method for measuring nano-gold mimetic peroxidase based urease and an inhibitor thereof. The method is characterized by comprising the following steps: coupling a urease catalysis urea reaction system with a nano-gold mimetic peroxidase-hydrogen peroxide-3,3',5,5'-tetramethylbenzidine dihydrochloride catalytic chromogenic reaction system, and combining with the changes of solution colors and ultraviolet absorption spectrum characteristics to directly measure the content of urease and the inhibitor thereof, wherein in a range of 1.8-90U / L, A450 and the urease concentration have a linear relationship, and the limit of detection is 1.8U / L; and by virtue of software fitting, IC50 of obtained acetohydroxamic acid of the urease inhibitor is 0.05mmoL / L. The method disclosed by the invention is simple and convenient to operate and high in sensitivity, and can be applied to the measurement of the content of urease and the inhibitor thereof in environment and life science systems as an analysis method.

The present invention provides a soy protein-based adhesive composition and a process for making the composition that may be used in particleboards and similar wood composite systems and that is formed from an aqueous mixture of calcium oxide, pine oil, protein, lignin, and acid. The preferred protein is an enzymatically-modified soy protein and the preferred enzyme is urease.

The present invention relates to methods of improving the environment within a poultry farming facility including reducing ammonia production in a poultry facility, inhibiting urease enzymes in poultry litter, reducing levels of pathogenic bacteria in poultry litter, improving productivity of poultry farms, reducing or preventing pododermatitis in poultry reared in mass production poultry facilities and controlling pests in poultry litter. Compositions, suitable for use in such methods, comprising at least one microorganism of the genus Bacillus and at least one biosurfactant wherein the biosurfactant is present in an amount of 2 mg / L to 7000 mg / L are also described.

The invention discloses preparation of N,S codoped graphene quantum dot having pH sensitive response and determination of urease activity. A preparation method comprises the steps that a carbon source compound and a sulfur source compound perform hydrothermal reaction, then a nitrogen source compound is added to obtain graphene quantum dot having pH response. The steps of the preparation method are simple, the used source materials are common, the cost is low, the yield is high, and the impurity amount is small. Therefore, popularization and application of the graphene quantum dot are promoted. The N,S codoped graphene quantum dot is stable in structure and rich in group on surface, has good fluorescence property and has good linearity in the pH range of 3-9. Due to the fact that OH- and NH<4+> are generated due to urea hydrolysis of urease, a pH value of a whole solution system is improved, the changing fluorescence intensity is further determined through the N,S codoped graphene quantum dot having the pH sensitive response and used for detecting the urease activity, and the lowest detection limit of the urease activity can be up to 2.5 U / L.

A pharmaceutical composition and method for use in inhibiting growth of cancer cells in a mammalian subject are disclosed. The composition includes a urease enzyme, and associated therewith, a chemical entity effective to enhance the delivery of the enzyme to cancer cells, when the composition is administered to the subject. Also disclosed are a method of enhancing the effectiveness of weakly basic anti-tumor compounds, a method assessing the presence, size or condition a solid tumor in a subject, and a gene therapy composition for treating a cancer in a subject.

The invention which discloses a special slow release fertilizer for corn zinc power and a preparation method thereof relates to the technical field of slow release fertilizers. The preparation method which follows a nutrient absorption rule of corn comprises the following steps: burdening heated short grained urea, monoammonium phosphate, potassium chloride, chelated zinc, a urease inhibitor, a nitration inhibitor and bentonite according to a prescribed ratio, fusing urea at a high temperature, mixing, nozzle-granulating, cooling, screening, metering, and packaging. The special fertilizer has the advantages of reasonable nutrient distribution ratio, corn photosynthesis promotion, effective improvement of the absorption utilization rate of the fertilizer, and production cost saving.

The invention discloses a urease inhibitor determination method based on a fluorescence gold nano cluster. The urease inhibitor determination method is characterized in that urea is catalyzed to generate ammonium and carbon dioxide by utilizing the specificity of urease, the pH value of the system can be increased through the newly generated ammonium, the fluorescence of the gold nano cluster protected by N-acetyl-L-cysteine is extinguished, the urease inhibitor can prevent the process that the urea is decomposed by the catalyzing of the urease, the extinguishment of the fluorescence is inhibited, and the urease inhibitor determination method can be used for detecting the urease inhibitor. An F650 value is determined, the inhabitation rate is calculated, and the contents of IC50 of cysteamine and p-benzoquinone, respectively 2.8mu mol / L and 11.9mu mol / L, can be obtained through the fitting by software. The urease inhibitor determination method can be used for the high-throughput screening of the urease inhibitor.

The invention discloses an efficient preparation method and applications of food-grade acid ureases, belonging to the technical field of biological engineering. The efficient expression and preparation methods and applications of acid ureases are realized successfully by using food-grade lactococcus lactis expression system, the enzyme activity of the acid ureases reaches 10200 U / L, and the enzyme activity of purified acid ureases is 325.6 U / mg. The specific enzyme activity of purified enzymes to an urethane substrate reaches 243.4 U / mg, and food-grade acid ureases which are prepared by using the method disclosed by the invention and added in a yellow rice or millet wine can efficiently degrade urea (the degradation rate per two days is 100%) and urethane (the degradation rate per three days is 72% %). Recombined acid ureases prepared by using the method disclosed by the invention can be applied to the removal of urea and urethane in fermented food, so that the residue problem of urethane in fermented food can be efficiently solved. The efficient preparation method and applications of food-grade acid ureases disclosed by the invention lay a foundation for the implementation of the industrialized production of food-grade acid ureases.

A dialysis apparatus for treating a patient with liver and / or kidney disease. In one embodiment a liver dialysis apparatus comprises an artificial liver. The artificial liver comprises a blood compartment, a vegetable protein compartment, and a clear dialysis compartment. In another embodiment the liver dialysis apparatus comprises cartridge made up of at least one layer of vegetable protein. In a further embodiment of the invention a dialysate regeneration device is provided which comprises at least one layer of vegetable protein. The vegetable protein is preferably a soy protein. The soy protein may be unmodified soy protein with urease enzyme activity, modified soy protein with or without urease activity, alone or in combination.

Slow-release fertilizer is produced by composting animal manure with a magnesium-rich compound The temperature of this mixture is maintained at 20-30° C. and the pH is maintained between 7-10. Urease is added to the mixture. Next, the mixture is inoculated with bacteria of the species Bacillus sphaericus, Bacillus globisporus, or Bacillus fusiformis. The mixture is then allowed to incubate for about 14 days to form magnesium ammonium phosphate. Optionally, phosphatase, an enzyme promoting the formation of phosphate from phosphorus-rich organic compounds, is added with the urease enzyme to increase the yield of magnesium-ammonium phosphate.

The invention relates to a compound deodorant special for livestock and poultry and a preparation method and application thereof. The compound deodorant comprises, by mass percentage, 3-4% of urease inhibitor, 5-10% of bacillus subtilis, 10-15% of clostridium butyricum, 1-2% of glucose oxidase, 10-13% of spider enzymes, 8-10% of fructo-oligosaccharide powder, and the balance corn starch. The sum of the contents of the components is 100%. The method for preparing the compound deodorant comprises a step 1 of first weighing glucose oxidase, the urease inhibitor and a certain amount of corn starch to perform premixing, and obtaining a preparation I for standby application; a step 2 of weighing and mixing bacillus subtilis, the fructo-oligosaccharide powder, clostridium butyricum and the spider enzymes, and obtaining a preparation II for standby application; a step 3 of fully mixing the preparation I and the preparation II, finally adding the auxiliary material corn starch to regulated mass, and obtaining the compound deodorant special for livestock and poultry. Various in vivo regulating agents are compounded scientifically and reasonably, the problems that an ordinary deodorant is low in deodorization efficiency, poor in safety and high in addition cost and the like are solved, the deodorization effect is improved, and the production performance of the livestock and poultry is improved.

The invention provides a method for preparing humic acid coated urea, which comprises the following steps of: dissolving 20 to 400 parts of urea in 40 to 200 parts of water at the temperature of between 20 and 60 DEG C, reacting with 40 to 200 parts of humic acid feed coal at the temperature of between 40 and 100 DEG C for 5 to 30 minutes, adding 10 to 100 parts of phosphate, continuously stirring for reacting for 30 to 120 minutes, performing solid-liquid separation, and evaporating and concentrating to obtain a humic acid-urea complex concentrated liquor, or drying to obtain a solid humic acid-urea complex; and spraying, infiltrating and drying by using coat drying equipment to obtain the humic acid coated urea. The humic acid feed coal has rich resources, and is cheap and readily available; a process flow is short, equipment is easy and convenient to operate, reaction conditions are mild, and the method is easy for production control; the production process is environment-friendly,pollution emission is avoided, and the method is suitable for large-scale industrial production and promotion and application; and the product has good water solubility and attractive appearance, caneffectively inhibit urease from decomposing the urea and improve the fertilizer efficiency of the urea and the utilization rate of nitrogen.