202 results about "Urease enzyme" patented technology

A sorbent hemodialysis system includes a dialyzer configured to receive a flow of clean dialysate from a reservoir and to output an unclean dialysate flow. The system also includes a sorbent component having a urease section and a sorbent section through which the unclean dialysate flow from the dialyzer passes, wherein the sorbent component removes urea from the dialysate. The system further comprises a membrane electrolyzer that receives at least a portion of said clean dialysate flow and separates the dialysate flow into an acidic component flow and a base component flow. A mixing conduit combines the base component flow from the membrane electrolyzer and an output dialysate solution from the urease section of the sorbent component to separate the dialysate solution into an ammonia gas amount and ammonia liquid amount. A gas vent is used to vent the ammonia gas amount, and the sorbent section with a suitable amount of zirconium phosphate (ZrP) removes the ammonia liquid amount from the unclean dialysate flow before flowing the clean dialysate to the reservoir. The system can further include a second mixing conduit upstream of the sorbent section of the sorbent component, the second mixing conduit combining the acidic component flow and the ammonia liquid amount in the dialysate solution to increase the pH of the dialysate solution to about 7.5 prior to returning to the reservoir.

An apparatus and method for replenishing urease in a sorbent cartridge for use in sorbent dialysis. The sorbent cartridge is configured to allow an amount of urease to be added to the sorbent cartridge. A urease solution can be injected into the sorbent cartridge to replenish the urease containing module, or solid urease can be added to the sorbent cartridge. The sorbent module can also comprise other, rechargeable, sorbent materials for removing toxins other than urea from spent dialysate.

An apparatus and method for replenishing urease in a sorbent cartridge for use in sorbent dialysis using urease pouches. The sorbent cartridge is configured to allow insertion of a urease pouch or injection of a urease solution into the sorbent cartridge containing a urease pouch. The sorbent module can also comprise other, rechargeable, sorbent materials for removing toxins other than urea from spent dialysate.

An apparatus and method for replenishing urease in a sorbent cartridge for use in sorbent dialysis. The system is configured to allow insertion of a urease pouch, injection of a urease solution, or addition of a urease cartridge, into a dialysis cabinet containing a dialysis flow loop. The urease can be dissolved and the resulting urease solution added to the sorbent cartridge in the flow loop to replenish the urease within the sorbent cartridge. The sorbent cartridge can also comprise other, rechargeable, sorbent materials for removing toxins other than urea from spent dialysate.

The invention discloses a long-acting synergistic controlled release fertilizer, which mainly comprises a nitrogen fertilizer, a phosphate fertilizer, a potash fertilizer, a biological inoculant, a urease inhibitor, a nitrification inhibitor and poly(aspartic acid). Specifically, in terms of weight percentage, the nitrogen fertilizer accounts for 10-60, the phosphate fertilizer accounts for 10-60, the potash fertilizer accounts for 10-60, an organic biological multielement compound agent accounts for 5-50, wherein the biological inoculant accounts for 6-8, a liquid binder accounts for 3-5, a filling material accounts for 10-20, livestock and poultry manure or humic acid accounts for 50-80, the urease inhibitor accounts for 0.1-0.15, the nitrification inhibitor accounts for 0.15-0.2, and the poly(aspartic acid) accounts for 6-20. The invention aims to overcome the defects in the prior art, ordinary fertilizers have the disadvantages of short effective period, unreasonable nutrient proportion, unscientific and unbalanced nutrient supply, especially the nitrogen fertilizer conflict is more prominent. The long-acting synergistic controlled release fertilizer is released along with the absorption rate of crops, fertilizer loss can be effectively reduced, and saving, yield increase, synergia, convenience, environmental protection and multiple objectives can be achieved, and agricultural sustainable development can be realized. The long-acting synergistic controlled release fertilizer is simple and feasible in application, and is suitable for China's actual conditions, thus being an inevitable course for the world fertilizer development in the 21st century.

The invention discloses a melt granulated polypeptide stable fertilizer and a preparation method thereof, relating to the field of compound fertilizers. The preparation method comprises the following steps: proportionally preparing urea, monoammonium phosphate, ammonium chloride, potassium chloride, compound urease inhibitor, compound nitrification inhibitor, polypeptide synergist and talcum powder; and heating the raw materials, mixing, carrying out spray granulation, cooling, screening, measuring and packaging to obtain the finished product. The stable fertilizer disclosed by the invention has the advantages of high utilization ratio, no pollution and obvious yield increase effect, promotes absorption of microelements, improves the quality of the crops, and enhances the stress tolerance.

The invention discloses a melt granulation polypeptide stability lost control compound fertilizer and a preparing method thereof, which relate to the technical field of compound fertilizers. The melt granulation polypeptide stability lost control compound fertilizer adopts urea, monoammonium phosphate, ammonium chloride, potassium chloride, zinc oxide, ferrous oxide, manganese sulfate, combined urease inhibitors, combined nitrification inhibitors, polypeptide synergist and fillings as raw matreials, and all the raw materials are dosed according to a specified ratio. The polypeptide stability lost control compound fertilizer product is obtained through processes such as mixing stirring, spray granulating, cooling curing, sieving, measuring and packing. The melt granulation polypeptide stability lost control compound fertilizer product has the advantages of improving fertilizer use ratio, being completely degraded without residuals and being high in absorbed efficiency.

The invention provides a special slow control/release fertilizer for an eggplant. The fertilizer is characterized by comprising, in terms of mass percent, 0.05% to 0.08% of n-butyl triamine thiophosphate, 0.05 to 0.08% of hydroquinone and 0.24 to 0.28% of dicyandiamide. According to the invention, the special slow control/release fertilizer is prepared by combining macro-element, medium-element and trace-element fertilizers like nitrogen, phosphorus, potassium, calcium, magnesium, molybdenum, boron, zinc and iron with organic fertilizers like high-quality decomposed rapeseed dregs, chicken manure and grass carbon, adding a urease inhibitor and a nitrification inhibitor and carrying out mixing; the special slow control/release fertilizer has a slow release effect in soil, is rich in a variety of nutrient elements needed in growth of the eggplant, can meet demands of the eggplant for nutrients in different growth and development phases for a long time and effectively improves output of the eggplant.

The invention discloses a pickle prolonged-effect-time compound fertilizer and a preparation method thereof. The pickle prolonged-effect-time compound fertilizer includes nitrogen fertilizer, phosphate fertilizer, potash fertilizer, urease inhibitor, nitrification inhibitor and fertilizer synergist, wherein the total mass fraction of the nitrogen fertilizer, the phosphate fertilizer and the potash fertilizer is not lower than 96%; the preparation method of the pickle prolonged-effect-time compound fertilizer includes the steps as follows: smashing urea to powder so as to btain urea powder; adding water for fully dissolving borax, thiourea, dicyandiamide and polyaspartic acid to obtain a mixed solution; spraying all the mixed solution into the urea powder and then mixing with calcium superphosphate and potassium chloride to obtain a mixture; adding a binder; and granulating the mixture and then drying so as to obtain products. The pickle prolonged-effect-time compound fertilizer has high utilization rate, long fertilizer effect time, using safety and lower relative cost, can meet primary fertilizer application of the pickle, and can obviously reduce the convent of nitrate in the pickle.

The present invention relates to methods of improving the environment within a poultry farming facility including reducing ammonia production in a poultry facility, inhibiting urease enzymes in poultry litter, reducing levels of pathogenic bacteria in poultry litter, improving productivity of poultry farms, reducing or preventing pododermatitis in poultry reared in mass production poultry facilities and controlling pests in poultry litter. Compositions, suitable for use in such methods, comprising at least one microorganism of the genus Bacillus and at least one biosurfactant wherein the biosurfactant is present in an amount of 2 mg/L to 7000 mg/L are also described.

A pharmaceutical composition and method for use in inhibiting growth of cancer cells in a mammalian subject are disclosed. The composition includes a urease enzyme, and associated therewith, a chemical entity effective to enhance the delivery of the enzyme to cancer cells, when the composition is administered to the subject. Also disclosed are a method of enhancing the effectiveness of weakly basic anti-tumor compounds, a method assessing the presence, size or condition a solid tumor in a subject, and a gene therapy composition for treating a cancer in a subject.

The invention which discloses a special slow release fertilizer for corn zinc power and a preparation method thereof relates to the technical field of slow release fertilizers. The preparation method which follows a nutrient absorption rule of corn comprises the following steps: burdening heated short grained urea, monoammonium phosphate, potassium chloride, chelated zinc, a urease inhibitor, a nitration inhibitor and bentonite according to a prescribed ratio, fusing urea at a high temperature, mixing, nozzle-granulating, cooling, screening, metering, and packaging. The special fertilizer has the advantages of reasonable nutrient distribution ratio, corn photosynthesis promotion, effective improvement of the absorption utilization rate of the fertilizer, and production cost saving.

The invention discloses a urease inhibitor determination method based on a fluorescence gold nano cluster. The urease inhibitor determination method is characterized in that urea is catalyzed to generate ammonium and carbon dioxide by utilizing the specificity of urease, the pH value of the system can be increased through the newly generated ammonium, the fluorescence of the gold nano cluster protected by N-acetyl-L-cysteine is extinguished, the urease inhibitor can prevent the process that the urea is decomposed by the catalyzing of the urease, the extinguishment of the fluorescence is inhibited, and the urease inhibitor determination method can be used for detecting the urease inhibitor. An F650 value is determined, the inhabitation rate is calculated, and the contents of IC50 of cysteamine and p-benzoquinone, respectively 2.8mu mol/L and 11.9mu mol/L, can be obtained through the fitting by software. The urease inhibitor determination method can be used for the high-throughput screening of the urease inhibitor.

The invention discloses an efficient preparation method and applications of food-grade acid ureases, belonging to the technical field of biological engineering. The efficient expression and preparation methods and applications of acid ureases are realized successfully by using food-grade lactococcus lactis expression system, the enzyme activity of the acid ureases reaches 10200 U/L, and the enzyme activity of purified acid ureases is 325.6 U/mg. The specific enzyme activity of purified enzymes to an urethane substrate reaches 243.4 U/mg, and food-grade acid ureases which are prepared by using the method disclosed by the invention and added in a yellow rice or millet wine can efficiently degrade urea (the degradation rate per two days is 100%) and urethane (the degradation rate per three days is 72% %). Recombined acid ureases prepared by using the method disclosed by the invention can be applied to the removal of urea and urethane in fermented food, so that the residue problem of urethane in fermented food can be efficiently solved. The efficient preparation method and applications of food-grade acid ureases disclosed by the invention lay a foundation for the implementation of the industrialized production of food-grade acid ureases.

A dialysis apparatus for treating a patient with liver and/or kidney disease. In one embodiment a liver dialysis apparatus comprises an artificial liver. The artificial liver comprises a blood compartment, a vegetable protein compartment, and a clear dialysis compartment. In another embodiment the liver dialysis apparatus comprises cartridge made up of at least one layer of vegetable protein. In a further embodiment of the invention a dialysate regeneration device is provided which comprises at least one layer of vegetable protein. The vegetable protein is preferably a soy protein. The soy protein may be unmodified soy protein with urease enzyme activity, modified soy protein with or without urease activity, alone or in combination.

The invention relates to a compound deodorant special for livestock and poultry and a preparation method and application thereof. The compound deodorant comprises, by mass percentage, 3-4% of urease inhibitor, 5-10% of bacillus subtilis, 10-15% of clostridium butyricum, 1-2% of glucose oxidase, 10-13% of spider enzymes, 8-10% of fructo-oligosaccharide powder, and the balance corn starch. The sum of the contents of the components is 100%. The method for preparing the compound deodorant comprises a step 1 of first weighing glucose oxidase, the urease inhibitor and a certain amount of corn starch to perform premixing, and obtaining a preparation I for standby application; a step 2 of weighing and mixing bacillus subtilis, the fructo-oligosaccharide powder, clostridium butyricum and the spider enzymes, and obtaining a preparation II for standby application; a step 3 of fully mixing the preparation I and the preparation II, finally adding the auxiliary material corn starch to regulated mass, and obtaining the compound deodorant special for livestock and poultry. Various in vivo regulating agents are compounded scientifically and reasonably, the problems that an ordinary deodorant is low in deodorization efficiency, poor in safety and high in addition cost and the like are solved, the deodorization effect is improved, and the production performance of the livestock and poultry is improved.

The invention provides a method for preparing humic acid coated urea, which comprises the following steps of: dissolving 20 to 400 parts of urea in 40 to 200 parts of water at the temperature of between 20 and 60 DEG C, reacting with 40 to 200 parts of humic acid feed coal at the temperature of between 40 and 100 DEG C for 5 to 30 minutes, adding 10 to 100 parts of phosphate, continuously stirring for reacting for 30 to 120 minutes, performing solid-liquid separation, and evaporating and concentrating to obtain a humic acid-urea complex concentrated liquor, or drying to obtain a solid humic acid-urea complex; and spraying, infiltrating and drying by using coat drying equipment to obtain the humic acid coated urea. The humic acid feed coal has rich resources, and is cheap and readily available; a process flow is short, equipment is easy and convenient to operate, reaction conditions are mild, and the method is easy for production control; the production process is environment-friendly,pollution emission is avoided, and the method is suitable for large-scale industrial production and promotion and application; and the product has good water solubility and attractive appearance, caneffectively inhibit urease from decomposing the urea and improve the fertilizer efficiency of the urea and the utilization rate of nitrogen.

A method for preparing a biochar-based fertilizer by using macadamia nut shells comprises the following steps: a raw material macadamia nut shells undergoes anaerobic pyrolysis to prepare modified biochar, and the biochar is mixed with cow dung biogas residues, a chemical fertilizer, a urease inhibitor and a nitration inhibitor to prepare the high-efficiency slow release fertilizer. The slow release fertilizer prepared in the invention has the advantages of simple production process, easily available raw material, low production cost, effective increase of the utilization of N, P and K, reduction of the loss of nutrients, improvement of the quality of soil, improvement of the quality of agricultural products, and facilitation of the benign development of the agricultural ecologic system.

The invention discloses a special water-soluble fertilizer for high-quality wheat and a preparation method of the special water-soluble fertilizer, and belongs to the technical field of agricultural fertilizers. The special water-soluble fertilizer for the high-quality wheat is prepared from the following raw materials in parts by weight: 60 to 75 parts of urea, 10 to 20 parts of monoammonium phosphate, 5 to 15 parts of potassium chloride, 1 to 10 parts of iron, 1 to 5 parts of manganese, 1 to 10 parts of boron, 5 to 15 parts of zinc, 1 to 5 parts of copper, 0.01 to 0.1 part of urease inhibitor and 0.01 to 0.1 part of nitrification inhibitor. The prepared water-soluble fertilizer has the characteristics of high dissolving speed, relatively high absorption and utilization rate, multiple nutrients and the like, can be applied to facility agriculture such as spraying, drip irrigation and the like, realizes water and fertilizer integration, improves the utilization efficiency of water andfertilizer, and achieves the effects of saving water, fertilizer and labor; meanwhile, the fertilizer can reduce the damage to soil and the environment caused by long-term use of a large amount of chemical fertilizer. In addition, the method can also be used for foliar fertilizer spraying and soilless culture.

Particulate fertilizer compositions that include nitrification inhibitors and urease inhibitors are described herein. The inhibitors are separated from each other by being in separate particles. Fertilizer particles in the composition include particles having a core-shell structure, with an inhibitor included in the core particle.

The invention relates to a non-enveloped slow release type enhancing compound fertilizer and a preparation method of the non-enveloped slow release type enhancing compound fertilizer. The non-enveloped slow release type enhancing compound fertilizer is prepared by the following components in percentage by weight: 0.50 to 1.50% of gamma-polyglutamic acid, 0.3 to 1.5% of a dissolution inhibitor, 0.3 to 0.6% of a nitrification inhibitor, 0.2 to 0.6% of a urease inhibitor, 0.5 to 1.2% of sodium dodecyl benzene sulfonate, and the balance of a compound fertilizer. The non-enveloped slow release type enhancing compound fertilizer provided by the invention is ecological, and environmental-friendly, has the capacity of water retention and fertilizer retention, is capable of reducing waste of the compound fertilizer, activating nutrient elements, improving an utilization rate of the compound fertilizer, slowly releasing nutrient, reducing application amount of the fertilizer, an environmetal pollution problem caused by largely using compound fertilizers is avoided, and agricultural production cost is saved.

A method for immobilizing bifunctional acidic urease through graphene oxide and chitosan microspheres is disclosed, and belongs to the technical field of enzyme preparations. The enzyme immobilizationamount, pH stability and temperature stability are obviously improved by utilizing a graphene oxide-chitosan crosslinked carrier. Complex application conditions of the bifunctional acidic urease areovercome, and the enzyme application rate is increased. The immobilized enzyme can be used for column packing conveniently so that a yellow wine treatment manner is facilitated. The immobilized enzymehas extremely low influences on volatile compounds in yellow wine, and has good urea and ethyl carbamate removing effects.

The invention relates to a stable long-acting compound fertilizer which comprises the following components in parts by weight: 50-130 parts of coated urea, 10-25 parts of calcium superphosphate, 5-20parts of ammonium chloride, 7-25 parts of potassium chloride, 10-30 parts of sweet potato type citric acid residue, 6-30 parts of vinasse, 3-18 parts of EDTA chelated zinc, 4-20 parts of amino acid chelated iron, 10-25 parts of plant growth regulator, 5-18 parts of urease inhibitor and 6-28 parts of an anti-caking agent. The compound fertilizer disclosed by the invention can meet the requirement of crops on nutrition, is reasonable in proportioning, slowly releases nutrients, improves the utilization rate of the fertilizer, promotes the crops to absorb nutrition, enhances the stress resistanceof the crops and reduces soil hardening and environmental pollution caused by fertilizer loss.