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Rapid detection reagent for helicobacter pylori

A Helicobacter pylori and detection reagent technology, applied in the field of detection, can solve problems such as complicated operation steps, poor accuracy, and error-prone

Pending Publication Date: 2022-04-05
南京康容健康科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] But this a kind of Helicobacter pylori detection kit and preparation method thereof also have some problems, such as detection speed is slow, often need to spend a large amount of time to wait for observation, just can find color change, and operation step is complicated, often needs operator to have extremely High level of experimentation, error-prone, poor test accuracy

Method used

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  • Rapid detection reagent for helicobacter pylori
  • Rapid detection reagent for helicobacter pylori

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Embodiment Construction

[0017] The present invention will be further explained below in conjunction with specific embodiments.

[0018] refer to figure 1 , embodiment one

[0019] A kind of Helicobacter pylori rapid detection reagent is proposed in the present embodiment, and detection reagent comprises sodium chloride, urea, indicator, ethanol solution, absorbent, additive and culture medium, and absorbent comprises nickelite, plant ash and salt, Additives include vinegar, disodium hydrogen phosphate and sodium hydroxide, and the amount of the internal components of the detection reagent is 15 parts of sodium chloride, 14 parts of urea, 10 parts of indicator, 60 parts of ethanol solution, 10 parts of absorbent, 9 parts of additives and 60 parts of culture medium, the ratio of hydrochlorite, plant ash and table salt in the absorbent is 4.5:2.5:3, and the ratio of vinegar, disodium hydrogen phosphate and sodium hydroxide in the additive is 6:2.5: 1.5. The ethanol solution is used to dissolve sodium ...

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Abstract

The invention belongs to the field of detection, particularly relates to a rapid helicobacter pylori detection reagent, and provides the following scheme aiming at the problems of low test speed and poor test accuracy in the prior art: the detection reagent comprises sodium chloride, urea, an indicator, an ethanol solution, an absorbent, an additive and a culture medium, the absorbent comprises bischofite, plant ash and salt, the additive comprises vinegar, disodium hydrogen phosphate and sodium hydroxide, and the helicobacter pylori is rapidly cultured by adopting an improved urease method and an international common ultra-rapid culture medium culture test, so that the helicobacter pylori rapidly secretes high-activity urease to decompose a substrate urea to generate ammonia gas, and the ammonia gas is converted into the high-purity helicobacter pylori. When ammonia is dissolved in water, the PH value is increased, the color of the indicator is changed, the helicobacter pylori in saliva, tartar or gastric mucosa tissue is indirectly dyed, whether the helicobacter pylori exists or not is judged, the testing speed is high, and the accuracy is high.

Description

technical field [0001] The invention relates to the technical field of detection, in particular to a rapid detection reagent for Helicobacter pylori. Background technique [0002] The patent with application number CN201911364775.6 discloses a Helicobacter pylori detection kit and its preparation method. The present invention discloses a Helicobacter pylori detection kit and its preparation method. The kit of the present invention can be used to detect Helicobacter pylori in saliva For Helicobacter infection, the reagent is simple, the cost is low, there is no harm, pain, side effect and contraindication to the human body, the operation is simple and fast, the sensitivity is high, and it is suitable for popularization and application. [0003] But this a kind of Helicobacter pylori detection kit and preparation method thereof also have some problems, such as detection speed is slow, often need to spend a large amount of time to wait for observation, just can find color chang...

Claims

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Application Information

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IPC IPC(8): C12Q1/04C12R1/01
Inventor 张广明
Owner 南京康容健康科技有限公司
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