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Method for obtaining polypeptide products from proteins

A protein and product technology, applied in the field of peptide product acquisition, can solve the problems of high production cost, low peptide purity, and low peptide acquisition rate, and achieve the effects of low cost, high peptide yield, and simple production process

Inactive Publication Date: 2017-10-13
ANHUI WANGJIABA ECOLOGICAL AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Peptides are some special fragment sequences contained in proteins, fundamentally have a positive effect on human health, and can promote health after oral administration. Egg protein is rich in protein, and the peptides obtained after enzymatic hydrolysis have good degrading properties. Blood pressure effect, but the current protein and peptide acquisition process mainly has the problems of high production cost, low peptide acquisition rate, and low purity of the obtained peptide

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1: A method for obtaining polypeptide products from proteins, specifically,

[0019] (1) Boil the eggs, get the protein, crush it to 60 mesh, add deionized water, then add malic acid to adjust the pH to 3, stir at a speed of 180r / min for 8 minutes, and then react in a water bath at 65°C 30 minutes;

[0020] (2) The recovered protein is dried at low temperature until the water content is 13%, and sent to a high-speed disperser for dispersing for 16 minutes to obtain a dispersed material;

[0021] (3) Evenly mix the dispersion material and sodium pyrophosphate at a weight ratio of 1000:4, add water to adjust the humidity of the dispersion material to 85%, inoculate Candida albicans with an inoculation amount of 2‰, and set the ambient temperature to 23°C. Fermentation treatment for 6 hours;

[0022] (4) Mix the dispersion material and water at a volume ratio of 1:6, centrifuge at a high speed of 5000r / min for 8 minutes, remove the residue, concentrate the supern...

Embodiment 2

[0023] Example 2: A method for obtaining polypeptide products from proteins, specifically,

[0024] (1) Boil the eggs, get the protein, crush it to 65 mesh, add deionized water, then add malic acid to adjust the pH to 3.2, stir at a speed of 200r / min for 10 minutes, and then react in a water bath at 70°C 32 minutes;

[0025] (2) The recovered protein is dried at low temperature until the water content is 15%, and sent to a high-speed disperser for dispersing for 18 minutes to obtain a dispersed material;

[0026] (3) Evenly mix the dispersion material and sodium pyrophosphate at a weight ratio of 1000:5, add water to adjust the humidity of the dispersion material to 88%, inoculate Candida albicans with an inoculation amount of 3‰, and set the ambient temperature to 25°C. Fermentation treatment for 7 hours;

[0027] (4) Mix the dispersion material and water at a volume ratio of 1:7, centrifuge at a high speed of 6000r / min for 10 minutes, remove the residue, concentrate the su...

Embodiment 3

[0028] Example 3: A method for obtaining polypeptide products from proteins, specifically,

[0029] (1) Boil the eggs, get the protein, crush it to 62 mesh, add deionized water, then add malic acid to adjust the pH to 3.1, stir at a speed of 190r / min for 9 minutes, and then react in a water bath at 68°C 31 minutes;

[0030] (2) The recovered protein is dried at low temperature until the water content is 14%, and sent to a high-speed disperser for dispersing for 17 minutes to obtain a dispersed material;

[0031] (3) Evenly mix the dispersion material and sodium pyrophosphate at a weight ratio of 1000:4.6, add water to adjust the humidity of the dispersion material to 87%, inoculate Candida albicans with an inoculation amount of 2.5‰, and set the ambient temperature to 24°C. Fermentation treatment for 6.5 hours;

[0032] (4) Mix the dispersion material and water at a volume ratio of 1:6.2, centrifuge at a high speed of 5500r / min for 9 minutes, remove the residue, concentrate ...

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Abstract

The invention relates to the technical field of acquisition of polypeptide products, and particularly relates to a method for acquiring polypeptide products from proteins. The method comprises the following steps: cooking eggs; acquiring proteins; crushing and adding deionized water; adjusting the pH value with malic acid; stirring for 8-10 min; drying the proteins recovered after reacting in water bath at a low temperature; dispersing at a high speed to obtain a dispersed material; uniformly mixing the dispersed material with sodium pyrophosphate at a weight ratio of 1000: 4-5; adding water to adjust the humidity of the dispersed material; inoculating saccharomyces albicans for fermentation treatment; mixing the dispersed material with the water uniformly; performing centrifugal treatment at a high speed; removing residues; concentrating supernatant; spray-drying a concentrated solution to obtain protein polypeptide products. The method for producing the protein polypeptide products, provided by the invention, has advantages of simple production process, low requirement on equipment, low cost, high yield of polypeptide and high purity; the obtained polypeptide products have significant effect on an aspect of decreasing blood pressure.

Description

technical field [0001] The invention relates to the technical field of obtaining polypeptide products, in particular to a method for obtaining polypeptide products from proteins. Background technique [0002] Functional food is called the food of the 21st century. Its unique function is that the active ingredients contained in it have a physiological regulation effect on the human body, so as to achieve the purpose of preventing, treating or assisting the treatment of diseases and promoting health. In recent decades, with the development of genetic The rapid development of biotechnology such as engineering, cell engineering, and enzyme engineering has greatly strengthened and improved the research level of functional foods; [0003] Peptides are some special fragment sequences contained in proteins, fundamentally have a positive effect on human health, and can promote health after oral administration. Egg protein is rich in protein, and the peptides obtained after enzymatic ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J3/04
CPCA23J3/04
Inventor 王金玲
Owner ANHUI WANGJIABA ECOLOGICAL AGRI
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