The invention discloses a loss preventing and hair strengthening nourishing solution. The nourishing solution comprises components in percentage by mass as follows: 0.0005%-0.002% of a hair follicle activation factor, 0.01%-1% of vitamins, 0.05%-5% of amino acid, 0.01%-1% of microelements, 0.01%-20% of a transdermal agent, 0.02%-10% of a conditioner and the balance of water. Meanwhile, the invention discloses a preparation method of the nourishing solution. The hair follicle activation factor is taken as the core effective component of the loss preventing and hair strengthening nourishing solution and matched with other auxiliary scalp nutrients, all the components cooperate and can balance microcirculatory system of scalp and reconstruct scalp barriers; the nourishing solution can activate resting cells, repair damaged hair follicle and rapidly stop loss and strengthen hair. Besides, the nourishing solution directly supplies hair growth nutrients through scalp hair follicle, can strengthen hair roots, activate activity of hair follicle, dredge blood vessels and promote rapid growth of hair, and has significant effects on soft hair, dry hair, splitting, no gloss, scalp itching, more dandruff, more hair oil, climacteric hair loss, alopecia areata and sebrrheic alopecia.