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ABO/RhD blood typing detection reagent card and preparation thereof

A technology for detecting reagents and blood types, which is applied in the field of blood type antibodies, can solve the problems of reagent sensitivity, uneven particle gap, misjudgment, etc., and achieve accurate and reliable detection results, maintain sufficient swelling, and ensure the effect of ionic strength

Inactive Publication Date: 2012-10-03
BEIJING KINGHAWK PHARMA
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  • Summary
  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

The card basically meets the detection requirements, but it lacks the RhD blood type test and self-agglutinin tube test control in the anti-typing test tube project. In some special cases, such as the presence of self-condensing hormones, it is easy to cause misjudgment of ABO blood type. And it is impossible to detect the D antigen in the RhD blood type at the same time; the selection and preparation of polyacrylamide dextran gel are easily affected by production operations and the environment, so that the gap between the particles is not uniform enough, which is easy to affect the sensitivity of the reagent, making the reagent Unstable quality

Method used

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  • ABO/RhD blood typing detection reagent card and preparation thereof
  • ABO/RhD blood typing detection reagent card and preparation thereof
  • ABO/RhD blood typing detection reagent card and preparation thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] 1. Preparation of gel suspension medium buffer: The formula of gel suspension medium buffer is as follows:

[0062]

[0063] The above reagents were dissolved in purified water, and the pH value was adjusted to 6.6-6.8.

[0064] 2. Gel preparation:

[0065] Select imported commercial cross-linked dextran G50 (Sephadex G50), fully swell the cross-linked dextran with the gel suspension medium buffer prepared above, and then wash with the suspension medium for 5 times to remove gel fragments and incomplete swelling Collect the gel that has been fully swollen.

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Abstract

The invention relates to an ABO / RhD blood typing detection reagent card and preparation of the ABO / RhD blood typing detection reagent card. The ABO / RhD blood typing detection reagent card is characterized in that the detection reagent card is provided with 8 micro-column gel tubes, wherein one micro-column gel tube contains gel and an anti-A monoclonal antibody of IgM property, one micro-column gel tube contains gel and an anti-B monoclonal antibody of IgM property, one micro-column gel tube contains gel and an anti-D RhD blood type monoclonal antibody of IgM property, and the rest five gel tubes contain gel suspending medium buffer solutions and gels.

Description

Technical field: [0001] The present invention relates to a blood type testing card and its preparation method, specifically refers to the use of micro-column gel technology to detect blood group antigens on the surface of human red blood cells, blood group antibodies in serum or plasma, and relates to the composition, preparation and application of the test card . Background technique: [0002] Human erythrocyte blood type is the earliest human blood type discovered, and it is a genetic marker of human beings, which is of great significance to human genetics, blood transfusion, tissue matching, organ transplantation and neonatal immunity. [0003] Since Landsteinor discovered the ABO blood type in 1900, Hoktoen first proposed the importance of blood type identification for blood transfusion therapy in 1907, and in 1908 Ottenberg further proposed the concept of donor-recipient blood cross-matching, further emphasizing the far-reaching significance of blood type identification...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/80
Inventor 李艳召戈军张志曹建荣
Owner BEIJING KINGHAWK PHARMA
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