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ABO/RhD blood group antigen detection reagent card and preparation method thereof

A technology for detecting reagents and blood group antigens, which is applied in the field of blood group antigen detection cards and its preparation, can solve problems such as uneven particle gaps, unstable reagent quality, and reagent sensitivity effects, so as to ensure ionic strength, maintain buffer range, and test results. Accurate and reliable results

Inactive Publication Date: 2012-09-19
BEIJING KINGHAWK PHARMA
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AI Technical Summary

Problems solved by technology

[0010] The above two domestic patents are not pure blood group antigen detection, and the use in the field of blood group antigen detection alone will easily cause waste of reagents and increase workload, and the selection and preparation of polyacrylamide dextran gel are easily affected by production operations and the environment. Influence, the gap between the particles is not uniform enough, it is easy to affect the sensitivity of the reagent, and the quality of the reagent is unstable

Method used

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  • ABO/RhD blood group antigen detection reagent card and preparation method thereof
  • ABO/RhD blood group antigen detection reagent card and preparation method thereof
  • ABO/RhD blood group antigen detection reagent card and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0058] 1. Preparation of gel suspension medium buffer: The formula of gel suspension medium buffer is as follows:

[0059]

[0060]

[0061] The above reagents were dissolved in purified water, and the pH value was adjusted to 6.6-6.8.

[0062] 2. Gel preparation:

[0063] Select imported commercial cross-linked dextran G50 (Sephadex G50), fully swell the cross-linked dextran with the gel suspension medium buffer prepared above, and then wash with the suspension medium for 5 times to remove gel fragments and incomplete swelling Collect the gel that has been fully swollen.

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Abstract

The invention relates to a preparation method of an ABO / RhD blood group antigen detection reagent card. Eight microcolumn gel tubes are arranged on the detection reagent card, wherein two gel tubes contain gel and anti-A monoclonal antibodies with an immunoglobulin m (IgM) property, two gel tubes contain gel and anti-B monoclonal antibodies with the IgM property, two gel tubes contain gel and anti-D monoclonal antibodies of RhD blood type with the IgM property, and two gel tubes contain a gel suspending medium buffer solution and gel.

Description

Technical field: [0001] The invention relates to a blood group antigen detection card and a preparation method thereof, in particular to the detection of blood group antigens on the surface of human red blood cells by micro-column gel technology, and relates to the composition, preparation and application of the detection card. Background technique: [0002] Human erythrocyte blood type is the earliest human blood type discovered, and it is a genetic marker of human beings, which is of great significance to human genetics, blood transfusion, tissue matching, organ transplantation and neonatal immunity. [0003] Since Landsteinor discovered the ABO blood type in 1900, Hoktoen first proposed the importance of blood type identification for blood transfusion therapy in 1907, and in 1908 Ottenberg further proposed the concept of donor-recipient blood cross-matching, further emphasizing the far-reaching significance of blood type identification for clinical blood transfusion signi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/80
Inventor 李艳召戈军张志曹建荣
Owner BEIJING KINGHAWK PHARMA
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