Broad spectrum antihuman globulin reagent assay card and preparation thereof

A technology of anti-human globulin and detection card, which is applied in the field of Coombs' test and anti-human globulin test. It can solve the problems of reagent sensitivity, insufficient particle gap, unstable reagent quality, etc., and achieve accurate and reliable detection results. Guaranteed ionic strength and the effect of maintaining the buffer range

Inactive Publication Date: 2012-09-26
BEIJING KINGHAWK PHARMA
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  • Summary
  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

[0006] The above domestic patents choose polyacrylamide dextran gel as the medium, and the preparation of the gel is easily affected by the production operation and the environment, so that the gap between the particles is not uniform enough, which easily affects the sensitivity of the reagent and makes the quality of the reagent unstable.

Method used

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  • Broad spectrum antihuman globulin reagent assay card and preparation thereof
  • Broad spectrum antihuman globulin reagent assay card and preparation thereof
  • Broad spectrum antihuman globulin reagent assay card and preparation thereof

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Experimental program
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Embodiment 1

[0060] 1. Preparation of gel suspension medium buffer: The formula of gel suspension medium buffer is as follows:

[0061]

[0062] The above reagents were dissolved in purified water, and the pH value was adjusted to 6.6-6.8.

[0063] 2. Gel preparation:

[0064] Select imported commercial cross-linked dextran G50 (Sephadex G50), fully swell the cross-linked dextran with the gel suspension medium buffer prepared above, and then wash with the suspension medium for 5 times to remove gel fragments and incomplete swelling Collect the gel that has been fully swollen.

[0065] 3. Antibody selection:

[0066] Choose broad-spectrum anti-human globulin reagents (anti-IgG, C 3 D).

[0067] The reagent reacts positively with anti-D sensitized erythrocytes of IgG nature, and with C 3 D antibody sensitized erythrocytes produce a positive reaction, and neither IgG antibody nor C 3 Antibody-D sensitized erythrocytes produce a negative reaction.

[0068] Broad-spectrum anti-human g...

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Abstract

The invention relates to a broad spectrum antihuman globulin reagent assay card and preparation thereof. The assay card is provided with 8 microcolumn gel tubes, wherein broad spectrum IgG (immunoglobulin globulin) and C3D antibody reagents are contained in 8 microcolumn gel tubes.

Description

Technical field: [0001] The invention relates to a blood type detection card and a preparation method thereof, which are mainly used for blood type cross-matching, irregular antibody screening, and irregular antibody identification. Antiglobulin test, Coombs' test. It relates to the composition, preparation and application of the detection card. Background technique: [0002] Micro-column gel technology was invented by Yves lapierre in 1984, and the patented product was introduced to the market in 1988 by Daamey, Switzerland. The principle is based on whether free red blood cells and aggregated red blood cells pass through the colloidal medium composed of gel particles with molecular sieve function, so that red blood cells in different states can be separated. Micro-column gel broad-spectrum anti-human globulin reagent detection card is a new product combining micro-column gel technology and anti-human globulin test method, which has been widely used in recent years. Comp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/80
Inventor 戈军李艳召张志曹建荣
Owner BEIJING KINGHAWK PHARMA
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