Rh blood type detecting card

A technology for detecting card and rh blood type, applied in the medical field, can solve the problems of inability to effectively protect the stability of antibodies, inability to balance sensitivity and specificity, and inability to fully and completely swell the gel, so as to ensure purity, ensure ionic strength, and ensure The effect of stability

Active Publication Date: 2015-04-29
合肥天一生物技术研究所有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, some manufacturers at home and abroad have produced Rh blood type test cards, but there are some shortcomings as follows: one is to use a single type of dextran gel or polypropylene dextran gel, which makes it difficult to balance sensitivity and specificity; Physiological saline or antibody buffer swells the gel, making the gel unable to fully swell completely and destroying the ionic strength of the reaction system; third, for gels with different antibodies added, the gel washing buffer is exactly the same, which cannot effectively protect the stability of the antibody

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A Rh blood type detection card includes a detection card containing six microcolumns, and the six microcolumns are respectively equipped with anti-D gel, anti-C gel, anti-c gel, anti-E gel and anti-e gel and blank gels, which were prepared by:

[0037] (1) Swelling of dextran gel:

[0038] Select Sephadex G-25, Sephadex G-50 and Sephadex G-75 to mix according to the mass ratio of 1:2:1 to obtain Sephadex mixture , and then add purified water according to the mass volume ratio of the dextran gel mixture and purified water as 1:3, and suspend and swell for 48 hours; then wash it with purified water three times to remove broken particles, and obtain uniform and complete Spherical curdlan gum particles;

[0039] (2) Preparation of antibody diluent:

[0040] Anti-D diluent: use phosphate buffered saline (PBS) as the base solution, which contains ethylenediaminetetraacetic acid dipotassium salt (EDTA dipotassium salt) 10g / L, bovine serum albumin 40g / L, the pH value of the ...

Embodiment 2

[0062] A Rh blood type detection card includes a detection card containing six microcolumns, and the six microcolumns are respectively equipped with anti-D gel, anti-C gel, anti-c gel, anti-E gel and anti-e gel and blank gels, which were prepared by:

[0063] (1) Swelling of dextran gel:

[0064] Select Sephadex G-75 and Sephadex G-100 to mix according to the mass ratio of 1:1 to obtain Sephadex mixture, and then follow the method of Sephadex mixture and purified water Add purified water at a mass volume ratio of 1:2, suspend and swell for 72 hours; then wash it with purified water three times to remove broken particles, and obtain gelatin particles with uniform size and complete spherical shape;

[0065] (2) Preparation of antibody diluent:

[0066] Anti-D diluent: use phosphate buffered saline (PBS) as the base solution, which contains ethylenediaminetetraacetic acid dipotassium salt (EDTA dipotassium salt) 15g / L, bovine serum albumin 350g / L, the pH value of the diluent 6...

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PUM

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Abstract

The invention provides an Rh blood type detecting card comprising a detecting card with six micro-columns, wherein the six micro-columns are internally and respectively filled with equivalent D-resistant gel, C-resistant gel, c-resistant gel, E-resistant gel, e-resistant gel and blank gel. The Rh blood type detecting card is prepared by using the following method comprising the steps of swelling dextran gels; preparing all antibody diluents; preparing all antibody working solutions; washing the gels; and subpackaging to form the Rh blood type detecting card. According to the Rh blood type detecting card, crosslinked dextran gels with different sizes and sieving ranges are mixed and swelled by using purified water, so that the specificity is effectively ensured, meanwhile, the Rh blood type detecting card has very high sensitivity, and the ion strength of a system is effectively ensured.

Description

technical field [0001] The invention relates to the medical field, in particular to a Rh blood type detection card. Background technique [0002] The Rh system is an extremely complex blood group system, second only to the ABO system in importance. Among them, D antigen is the most important antigen in the Rh blood group antigen system. If D antigen exists on the human red blood cell membrane, it is RhD positive, and if D antigen does not exist, it is RhD negative. In addition to the D antigen, the C, c, E, and e antigens are also the main antigens in the Rh system, which have strong clinical significance. In addition, since the vast majority of irregular antibodies belong to the antibodies of the Rh blood group system, after transfusion of blood containing the corresponding antigens, it can cause severe immune hemolytic transfusion reactions, and IgG antibodies of the Rh system have a strong ability to pass through the placenta. Destruction of fetal red blood cells with...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/80
CPCG01N33/531G01N33/80
Inventor 何流张艳徐寅生尹培培
Owner 合肥天一生物技术研究所有限责任公司
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