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Serum protein marker for early screening and diagnosis of esophageal squamous carcinoma, kit and detection method

A serum protein and esophageal squamous cell carcinoma technology, applied in the field of biomedicine, can solve the problems of poor diagnostic ability of the diagnostic index tumor, the application of a single anti-TAA autoantibody, and the low frequency of occurrence, and achieves low cost, high sensitivity, and good reference. effect of value

Active Publication Date: 2020-01-21
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Numerous studies have also provided the basis and feasibility for anti-TAAs autoantibodies to be used in the early diagnosis of esophageal cancer. However, based on the complexity of the tumorigenesis process, the ability of a single diagnostic index for tumor diagnosis is relatively poor, and generally cannot meet the requirements of clinical tumor diagnosis. The need for diagnosis, as studies have shown, the frequency of a single anti-TAAs autoantibody in the serum of patients with esophageal cancer is very low, generally no more than 20%, which limits the application of a single anti-TAA autoantibody in tumor diagnosis

Method used

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  • Serum protein marker for early screening and diagnosis of esophageal squamous carcinoma, kit and detection method
  • Serum protein marker for early screening and diagnosis of esophageal squamous carcinoma, kit and detection method
  • Serum protein marker for early screening and diagnosis of esophageal squamous carcinoma, kit and detection method

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experiment example

[0050] 1 Preparation of serum samples

[0051] 1.1 Serum samples for protein chip experiments

[0052] Patients with primary esophageal squamous cell carcinoma (esophageal squamous cell carcinoma diagnosed by pathology) were collected in Beijing You'an Hospital and the First Affiliated Hospital of Zhengzhou University, with patient consent and approval by the Institutional Review Board and the Hospital Ethics Committee. For all samples, 5-10mL of whole blood was collected in red-headed blood collection tubes. After standing at room temperature for 2 hours, centrifuge at 1000xg for 15 minutes, take the supernatant, divide each sample into several copies, label them, and store them in a low-temperature refrigerator at -80°C. , avoid repeated freezing and thawing.

[0053] According to epidemiological analysis, 86 cases of primary esophageal squamous cell carcinoma sera and 50 normal control sera from You'an Hospital were collected for preliminary microarray screening. Among th...

Embodiment

[0116] A serum protein marker for early screening and diagnosis of esophageal squamous cell carcinoma, the serum protein marker is a combination of proteins encoded by P53, GNA11, GNAS, EGFR, SRSF2, MEN1 and DAXX genes;

[0117] The protein encoded by the P53 gene has the amino acid sequence shown in SEQ ID NO.1;

[0118] The protein encoded by the GNA11 gene has the amino acid sequence shown in SEQ ID NO.2;

[0119] The protein encoded by the GNAS gene has the amino acid sequence shown in SEQ ID NO.3;

[0120] The protein encoded by the EGFR gene has the amino acid sequence shown in SEQ ID NO.7;

[0121] The protein encoded by the SRSF2 gene has the amino acid sequence shown in SEQ ID NO.9;

[0122] The protein encoded by the MEN1 gene has the amino acid sequence shown in SEQ ID NO.10;

[0123] The protein encoded by the DAXX gene has the amino acid sequence shown in SEQ ID NO.11.

[0124] A kit, including serum protein markers for early screening and diagnosis of esophagea...

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Abstract

The invention discloses a serum protein marker for early screening and diagnosis of esophageal squamous carcinoma, and belongs to the field of biomedical technology. The serum protein marker is any one or a combination of two or more of proteins encoded by P53, GNA11, GNAS, PTEN, ACVR1B, FBXW7, EGFR, PDGFRA, SRSF2, MEN1, DAXX or CASP8 genes. Based on the role played by cancer driving genes in tumorigenesis and development, a human protein chip encoded by 138 cancer driving genes is customized, the human protein chip contains 180 human-derived recombinant proteins for screening out potential markers capable of diagnosing caners or other markers capable of characterizing cancers, early detection serum markers of the esophageal squamous carcinoma are primarily screened out by the protein chip, and then are verified by an ELISA indirect method experiment, a group of joint serum protein markers of esophageal squamous carcinoma for the early screening and diagnosis of the esophageal squamouscarcinoma is screened out at last for assisting the clinical diagnosis of the esophageal squamous carcinoma, so that the serum protein marker has better reference value.

Description

technical field [0001] The invention belongs to the technical field of biomedicine. Background technique [0002] Esophageal cancer (esophageal carcinoma, EC) is one of the common malignant tumors of the digestive tract in humans. From the perspective of tissue type, esophageal cancer is mainly divided into esophageal squamous cell carcinoma (esophageal squamous cell carcinoma) and esophageal adenocarcinoma. According to the latest data on global tumor prevalence (Globocan 2018) released by the Institute of Cancer Research, there were 572,000 cases of esophageal cancer worldwide in 2018, ranking seventh among malignant tumors; 509,000 deaths from esophageal cancer worldwide during the same period, ranking first among malignant tumors. The sixth place in death, the incidence of esophageal cancer can vary by 2-3 times in different regions of the world. China is one of the five regions with the highest incidence of esophageal cancer in the world. About 53% of the new cases of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/536G01N33/574G01N21/31
CPCG01N33/536G01N33/57407G01N33/57484G01N21/31
Inventor 张建营王鹏叶华代丽萍史健翔王晓孙桂英姜国忠赵志华
Owner ZHENGZHOU UNIV
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