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Newborn ABO/Rh blood grouping reagent card and preparation method thereof

A neonatal and reagent card technology, applied in the composition of ABO/Rh blood typing reagent cards, neonatal blood typing, and preparation fields, can solve the problems of ABO/Rh blood typing errors and reduce the accuracy of ABO/Rh blood typing, etc. Accelerate the reaction speed, shorten the incubation time, and the effect of excellent specificity

Active Publication Date: 2012-06-20
JIANGSU ZHONGSHENG MEDICAL DIAGNOSTIC REAGENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the existing card only detects RhIgM antibody and does not detect Rh IgG antibody, which reduces the accuracy of ABO / Rh blood typing and easily leads to ABO / Rh blood type errors

Method used

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  • Newborn ABO/Rh blood grouping reagent card and preparation method thereof
  • Newborn ABO/Rh blood grouping reagent card and preparation method thereof
  • Newborn ABO/Rh blood grouping reagent card and preparation method thereof

Examples

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Effect test

Embodiment Construction

[0043] A method for preparing a neonatal ABO / Rh blood type identification reagent card is characterized in that it comprises the following steps:

[0044] 1) Preparation of gel suspension buffer

[0045] The buffer formula is as follows:

[0046] 20 mL of 0.15M phosphate buffered saline

[0047] Sodium chloride 1.75g

[0048] Glycine 18g

[0049] Among them, 20mL of 0.15M phosphate buffer is made of 11.3mL of 0.15MKH 2 PO 4 and 8.7 mL of 0.15M Na 2 HPO 4 composition;

[0050] Dissolve the above reagents in 800 ml of distilled water, adjust the pH to 6.7 with 1M sodium hydroxide, and finally set the volume to 1 liter;

[0051] Add 0.6 g of methyl p-hydroxybenzoate and 0.13 g of propyl p-hydroxybenzoate, add bovine serum albumin, the addition amount is to make the final concentration of bovine serum albumin be 3%; obtain gel suspension buffer;

[0052] 2) Gel preparation

[0053] Select dextran gel, the particle size is 20-50 microns, fully soak with the gel suspension...

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Abstract

The invention discloses a newborn ABO / Rh blood grouping reagent card and a preparation method thereof. Eight microcolumn gel tubes are provided, including: a gel tube containing a monoclonal anti-A antibody with an Ig (Immunoglobulin) M property, a gel tube containing a monoclonal anti-B antibody with the Ig M property, a gel tube containing a monoclonal anti-AB antibody with the Ig M property, a gel tube containing a monoclonal anti-D antibody with the Ig M property, a gel tube containing a monoclonal anti-D antibody with the Ig M property and an Ig G property, a gel tube which contains gel suspending medium and is used as negative control, a gel tube containing an anti-Ig G reagent and a gel tube containing the anti-Ig G reagent and an anti-C3d reagent. The reagent card disclosed by the invention has the advantages that standardization is provided for newborn ABO / Rh (D) blood grouping and conditions are created for accurate determination of ABO / Rh (D) blood group and early diagnosis and treatment of hemolytic diseases of newborn.

Description

technical field [0001] The invention relates to a newborn blood typing and its application. More specifically, the present invention relates to the composition, preparation method and application of an ABO / Rh blood typing reagent card, and relates to the field of early diagnosis of neonatal hemolytic disease and blood transfusion medicine. Background technique [0002] Hemolytic disease of the newborn (HDN for short) is an immune hemolytic disease that occurs in fetuses and early neonates. The disease is caused by incompatibility of blood types of mother and baby. When the dominant antigen inherited by the fetus from the father is just lacking in the mother, the antigen enters the mother through the placenta during pregnancy, stimulating the mother to produce corresponding immune antibodies, which can be strengthened by being stimulated by re-pregnancy. The immune antibodies corresponding to fetal antigens in the mother can enter the fetus through the placenta and combine ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/80
Inventor 朱晓霞王惠民施秀英
Owner JIANGSU ZHONGSHENG MEDICAL DIAGNOSTIC REAGENT
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