ABO-CDE blood type grouping reagent card and preparation method thereof

An ABO-CDE, blood type identification technology, applied in biological testing, material inspection products, etc., can solve the problems of wrong ABO blood type, lack of Rh type, reducing the accuracy of ABO blood type type, etc., to achieve the effect of strong buffering capacity

Inactive Publication Date: 2012-01-25
JIANGSU ZHONGSHENG MEDICAL DIAGNOSTIC REAGENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At home and abroad, the gel that some manufacturers have adopted is dextran gel. The diameter of the gel particles is about 20-50 microns, and the ABO / Rh blood typing test for ABO blood typing and Rh series typing has been produced. Reagent cards, but the existing cards only perform ABO positive typing and do not perform ABO reverse typing, which reduces the accuracy of ABO blood typing and easily leads to ABO blood typing errors
In addition, the existing ABO / Rh blood type test card only carries out the identification of D (Rh1), but does not have the finalization of the Rh (Rh2, Rh3) series

Method used

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Examples

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Embodiment Construction

[0042] A preparation method for ABO-CDE blood type identification reagent card, comprises the following steps:

[0043] 1) Preparation of gel buffer

[0044] The buffer formula is as follows:

[0045]20 mL of 0.15M phosphate buffered saline

[0046] Sodium chloride 1.75g

[0047] Glycine 18g

[0048] Wherein 20mL of 0.15M phosphate buffer was mixed with 11.3mL of 0.15M KH 2 PO 4 and 8.7 mL of 0.15M Na 2 HPO 4 composition;

[0049] Dissolve the above reagents in 800 ml of distilled water, adjust the pH to 6.7 with 1M sodium hydroxide, and finally adjust the volume to 1 liter; add 0.6 g of methyl p-hydroxybenzoate and 0.13 g of propyl p-hydroxybenzoate, and add bovine serum albumin , the added amount is to make the final concentration of bovine serum albumin be 3%; obtain the gel suspension buffer;

[0050] 2) Gel preparation

[0051] Select dextran gel with a particle size of 20-50 microns, fully soak with the gel buffer prepared in step 1), and then wash with the buf...

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Abstract

The invention discloses an AB / CDE blood type grouping reagent card. The reagent card is provided with eight microcolumn gel tubes, namely a gel tube containing an immune globulin (IgM) monoclonal anti-A antibody, a gel tube containing an IgM monoclonal anti-B antibody, a gel tube containing an IgM monoclonal anti-D antibody, a gel tube containing an IgM monoclonal anti-C antibody, a gel tube containing an IgM monoclonal anti-E antibody, a gel tube which is used for negative control and contains a gel buffer solution and two gel tubes which are used for reverse typing and contain gel buffer solutions. The invention provides standard for the ABO-CDE blood type grouping; and the ABO-CDE blood type grouping reagent card which conforms to standard can be used in all hospitals and blood transfusion services to accurately group ABO-CDE blood types, so conditions are created for guaranteeing transfusion safety.

Description

technical field [0001] The invention relates to the preparation and application of a blood type typing card. More specifically, the present invention relates to the composition, preparation method and application of an ABO-CDE blood typing reagent card, and relates to the field of blood transfusion medicine. Background technique [0002] The ABO blood group system and the Rh blood group system are the two most important blood group systems for humans. Their high immunogenicity makes them have important clinical significance, especially closely related to clinical emergency blood transfusion and organ transplantation. Therefore, research on ABO blood group and Rh blood group antibodies is of great significance for clinical blood group identification, blood group theory research and the application of blood group antibodies to prevent and treat hemolytic disease of newborns. [0003] Since the discovery of ABO blood type in 1900, Hoktoen first proposed the importance of blood...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/80
Inventor 朱晓霞王惠民施秀英
Owner JIANGSU ZHONGSHENG MEDICAL DIAGNOSTIC REAGENT
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