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Forward and reverse ABO typing and RhD blood type detecting card

A positive and negative stereotyping and detection card technology, applied in the medical field, can solve the problems of inability to effectively protect the stability of antibodies, the inability to balance sensitivity and specificity, and the inability to fully swell the gel, so as to ensure purity, ionic strength, The effect of ensuring stability

Active Publication Date: 2015-04-29
合肥天一生物技术研究所有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, some manufacturers at home and abroad have produced ABO positive and negative typing and RhD blood type test cards, but there are some shortcomings: one is to use a single type of dextran gel or polypropylene dextran gel, which makes the sensitivity and specificity Can not take into account; the second is to use normal saline or antibody buffer to swell the gel, so that the gel cannot be fully swollen, and destroy the ionic strength of the reaction system; the third is to add different antibodies to the gel, the gel washing buffer is exactly the same, Unable to effectively protect antibody stability

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A detection card for ABO positive and negative typing and RhD blood type, including a detection card containing six microcolumns, the first three microcolumns in the six microcolumns are equipped with equal amounts of anti-A gel, anti-B gel and anti-B gel respectively. Anti-D gel, and the remaining three microcolumns were filled with an equal amount of blank gel, which was prepared by the following method:

[0033] (1) Swelling of dextran gel:

[0034] Select Sephadex G-25, Sephadex G-50 and Sephadex G-75 to mix according to the mass ratio of 1:2:1 to obtain Sephadex mixture , and then add purified water according to the mass volume ratio of the dextran gel mixture and purified water as 1:3, and suspend and swell for 48 hours; then wash it with purified water three times to remove broken particles, and obtain uniform and complete Spherical curdlan gum particles;

[0035] (2) Preparation of antibody diluent:

[0036] Anti-A diluent: use physiological saline as the bas...

Embodiment 2

[0052] A detection card for ABO positive and negative typing and RhD blood type includes a detection card containing six microcolumns, and the first three microcolumns in the six microcolumns are equipped with equal amounts of anti-A gel, anti-B gel and anti-B gel respectively. Anti-D gel, and the remaining three microcolumns were filled with an equal amount of blank gel, which was prepared by the following method:

[0053] (1) Swelling of dextran gel:

[0054] Select Sephadex G-75 and Sephadex G-100 to mix according to the mass ratio of 1:1 to obtain Sephadex mixture, and then follow the method of Sephadex mixture and purified water Add purified water at a mass volume ratio of 1:2, suspend and swell for 72 hours; then wash it four times with purified water to remove broken particles, and obtain gelatin particles with uniform size and complete spherical shape;

[0055] (2) Preparation of antibody diluent:

[0056] Anti-A diluent: use physiological saline as the base fluid, w...

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PUM

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Abstract

The invention discloses a forward and reverse ABO typing and RhD blood type detecting card comprising a detecting card with six micro-columns, wherein the first three micro-columns in the six micro-columns are respectively filled with equivalent A-resistant gel, B-resistant gel and D-resistant gel, and the left three micro-columns are filled with equivalent blank gel. The forward and reverse ABO typing and RhD blood type detecting card is prepared by using the following method comprising the steps of swelling dextran gels; preparing all antibody diluents; preparing all antibody working solutions; washing the gels; and subpackaging to form the RhD blood type detecting card. According to the forward and reverse ABO typing and RhD blood type detecting card, crosslinked dextran gels with different sizes and sieving ranges are mixed and swelled by using purified water, so that the specificity is effectively ensured, meanwhile, the forward and reverse ABO typing and RhD blood type detecting card has very high sensitivity, and the ion strength of a system is effectively ensured.

Description

technical field [0001] The invention relates to the medical field, in particular to an ABO positive and negative typing and RhD blood type detection card. Background technique [0002] There are many blood group antigen systems on the human erythrocyte membrane, among which the ABO blood group antigen system is the most important, followed by the Rh blood group antigen system. This is because the correct identification of ABO blood type is the guarantee of safe blood transfusion. If the ABO blood type is incompatible, the blood transfusion will almost without exception cause serious symptoms of immediate hemolytic transfusion reaction. There are four most common phenotypes of ABO blood type: A, B, AB, and O. The ABO blood typing test consists of a positive typing test and a negative typing test. Only when the positive and negative typing results match, can the ABO blood type be correctly judged. [0003] In the Rh blood group antigen system, the D antigen is the most impo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/80
CPCG01N33/80
Inventor 何流张艳徐寅生尹培培
Owner 合肥天一生物技术研究所有限责任公司
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