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Preparation method of chitosan hydrogel

A technology of hydrogel and chitosan, which is applied in the field of preparation of chitosan hydrogel, can solve the problems of too high and cytotoxicity, etc., and achieves the effects of simple method, precise control of gelation time, and easy production.

Active Publication Date: 2020-01-17
TONGJI UNIV
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the disadvantage of this method is that it requires a high concentration of phosphate, which is cytotoxic due to its high osmotic pressure, thus limiting the clinical application of this gelation method.

Method used

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  • Preparation method of chitosan hydrogel
  • Preparation method of chitosan hydrogel

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preparation example Construction

[0027] The invention discloses a preparation method of a chitosan hydrogel. Adding urea and urease into a chitosan solution can uniformly increase the pH value of the chitosan solution to make it gel and obtain the chitosan hydrogel.

[0028] refer to figure 1 , add 1-50mg / L phenol red to the chitosan solution as a pH indicator, before adding urea and urease, the solution is yellow, after adding urea and urease, the solution turns into a gel state and is purple .

Embodiment approach

[0029] The present invention provides an embodiment, comprising the following steps:

[0030] 1. Prepare 0.1-0.2M hydrochloric acid / acetic acid (1-50mg / L phenol red can be added as a pH indicator);

[0031] 2. Dissolve chitosan in acetic acid or hydrochloric acid to prepare a chitosan solution with a concentration of 1-5g / 100ml;

[0032] 3. Dissolving urease in PBS to prepare 5-1000U / ml urease solution;

[0033] 4. Dissolving urea (urea) in deionized water to prepare 8-10M urea solution;

[0034] 5. Mix the chitosan solution and the urease solution evenly, then add the urea solution and mix evenly to prepare chitosan enzymatic physical crosslinking hydrogel.

Embodiment 1

[0036] The present embodiment starts with the chitosan solution whose concentration is 2.5g / 100ml in 1ml, and the addition amount of urease is respectively 3.75, 7.5, 15, 30, 600U / ml through investigation, and the addition amount of urea is respectively 30, 37.5, 45 , 52.5, 60mM, the gelation time of chitosan enzymatically cross-linked hydrogel, the results are as follows figure 2 As shown, the test tube inversion method was used to detect the gelation time of chitosan enzymatically cross-linked hydrogel. Put the prepared solution in the centrifuge tube in a constant temperature metal bath or water bath, and use a stopwatch to time the gel time. The gel time is the time between the sol flowing in the centrifuge tube and the centrifuge tube not flowing after being inverted.

[0037] From figure 2 It can be seen that within a certain range, the gelation time of chitosan enzymatically physically crosslinked hydrogel can be precisely regulated by adjusting the concentration of ...

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Abstract

The invention relates to a preparation method of chitosan hydrogel. The preparation method comprises the following steps: adding urea and urease into a chitosan solution, and increasing the pH value of the chitosan solution to gelate the chitosan solution, so that the chitosan hydrogel is obtained. Compared with the prior art, the method provided by the invention is simple and is easy for manufacturing, and the gelation time of the chitosan enzymatic physical-crosslinking hydrogel can be accurately regulated and controlled by adjusting concentrations of urea and urease.

Description

technical field [0001] The invention belongs to the technical field of biomaterials, in particular to a preparation method of chitosan hydrogel. Background technique [0002] Among many biomaterials, chitosan has received more and more attention in the fields of tissue engineering and regenerative medicine. Chitosan is the deacetylation product of chitin, which is widely found in shrimp, crab, fungi and algae, and its content is extremely rich. Chitosan has a wide range of sources and is cheap and easy to obtain. Because of its good biocompatibility, biodegradability, promotion of tissue regeneration, anti-inflammatory and antibacterial effects, it is a class of biomaterials approved by the FDA and has been widely used in the field of biomedicine. [0003] Chitosan can be made into powder, film, fiber, hydrogel and porous material. The injectable in situ hydrogel has the advantages of strong plasticity, can fill various irregular defects, and less trauma, and has the bion...

Claims

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Application Information

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IPC IPC(8): C08J3/24C08J3/075C08L5/08C08K5/21
CPCC08J3/075C08J3/24C08J2305/08C08K5/21
Inventor 闫香珍
Owner TONGJI UNIV
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