Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Gel reagent for rapid screening of urease activity, kit and rapid screening method

A technology of urease and kit, which is applied in the field of medical testing, can solve the problems of low equipment penetration rate and mercury content, and achieve the effect of simple operation, high convenient application performance, intuitive and rapid detection

Inactive Publication Date: 2021-07-09
许奕鹏
View PDF3 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Nessler's reagent contains mercury, and Raman spectroscopy needs to use toxic reagents such as silver nitrate, and the equipment penetration rate is low, so neither method has been widely used

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Gel reagent for rapid screening of urease activity, kit and rapid screening method
  • Gel reagent for rapid screening of urease activity, kit and rapid screening method
  • Gel reagent for rapid screening of urease activity, kit and rapid screening method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0023] In the examples 1-25, gel reagents with different urea concentrations and different phenol concentrations are provided, and the concentrations are measured in mg / ml. The formulations of each component are shown in Table 1, and are measured in volume (ml).

[0024] Table 1 embodiment 1~25 each component formula of gel reagent forms

[0025]

[0026]

[0027] A method for rapid screening of urease activity in the present embodiment is carried out according to the following steps:

[0028] a. Use a toothpick or cotton swab to collect an appropriate amount of samples (saliva, tartar, biopsy stomach, etc.);

[0029] b. push the sample from the tweezers to below the surface of the gel reagent in the kit with a toothpick or cotton swab, so that the sample is exposed to the gel reagent as much as possible, and ensure that the biopsy specimen is completely immersed in the gel reagent;

[0030] c. Stand still for 2-3 minutes, and visually observe the color change of the ge...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a gel reagent for rapid screening of urease activity, a kit and a rapid screening method, and belongs to the field of medical detection. The gel reagent for rapid screening of urease activity comprises 0.2-0.3 part by volume of urea; 4-8 parts by volume of phenol red; 0.02-0.1 part by volume of a proclin300; 2-10 parts by volume of a pH buffer solution; and 5-10 parts by volume of agar gel, whereinthe concentration of urea is greater than or equal to 7mg / ml, and the concentration of phenol red is greater than or equal to 30 * 10 <-3 > mg / ml. According to the detection method, a reaction product of urease and urea in saliva or a biopsy specimen is utilized to trigger corresponding index change so as to measure the urease activity. The detection steps: are as follows collecting a proper amount of samples by using an applicator device, pushing the whole samples from tweezers to a position below the surface of gel in the kit by using the applicator device, exposing the samples in the gel as many as possible, standing for 2-3 minutes, observing the color change of the gel by a visual method, determining that urease is positive if the gel becomes red, and determining that urease is negative if the color is not changed.

Description

technical field [0001] The invention belongs to the field of medical detection, and in particular relates to a gel reagent for rapid screening of urease activity, a kit and a rapid screening method. Background technique [0002] Dr. Robin Warren and Marshall first cultured this bacterium from a biopsy of the gastric antrum in 1982, calling it Campylobacter-like bacteria. Later, this genus was named Helicobacter pylori, which means spiral or spiral-shaped bacteria. Helicobacter pylori has been shown to cause active chronic gastritis and is a risk factor for gastric cancer and mucosa-associated lymphoid (MALT) lymphoma. Helicobacter pylori parasitizes in the human digestive tract, usually exists in the epithelium of the gastric mucosa, and can also multiply in the oral cavity. The urease produced by Helicobacter pylori can quickly enzymatically decompose urea to produce alkaline ammonia, so Helicobacter pylori is less susceptible to gastric acid attack in the stomach. [00...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 许奕鹏杨晓辉
Owner 许奕鹏
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products