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Lactobacillus acidophilus LA-10A capable of inhibiting helicobacter pylori and application thereof

A Helicobacter pylori, LA-10A technology, applied in the field of microorganisms, can solve the problem of incomplete determination of urease activity, and achieve the effect of preventing Helicobacter pylori infection

Active Publication Date: 2020-05-08
THANKCOME BIOLOGICAL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Patent CN102174450A discloses a screening method for Lactobacillus plantarum strains specifically for HP, but for the inhibition of gastric mucosal colonization ability of the most important strains of HP strains in vivo, it uses an incomplete and indirect method for evaluating the activity of urease

Method used

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  • Lactobacillus acidophilus LA-10A capable of inhibiting helicobacter pylori and application thereof
  • Lactobacillus acidophilus LA-10A capable of inhibiting helicobacter pylori and application thereof
  • Lactobacillus acidophilus LA-10A capable of inhibiting helicobacter pylori and application thereof

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Embodiment 1

[0055] Embodiment 1: strain preservation

[0056] The invention relates to a Lactobacillus acidophilus LA-10A, which is preserved in the China Center for Type Culture Collection, with a preservation number of CCTCC NO: M2019012.

Embodiment 2

[0057] Embodiment 2: Lactobacillus acidophilus LA-10A characteristic of the present invention

[0058] Has the following characteristics:

[0059] 2.1 At pH 2.0, it can effectively survive for 4 hours in a simulated gastric acid environment with pepsin added;

[0060] 2.2 Under liquid culture conditions, it has an inhibitory effect on the proliferation of Helicobacter pylori;

[0061] 2.3 Under liquid culture conditions, it has an inhibitory effect on the urease activity of Helicobacter pylori;

[0062]2.4 The LA-10A strain and its fermentation supernatant have an inhibitory effect on the solid culture of Helicobacter pylori;

[0063] 2.5 In vitro, it has an inhibitory effect on the adhesion (colonization) ability of Helicobacter pylori in gastric epithelial cells;

[0064] 2.6 Tests have shown that it can reduce or prevent Helicobacter pylori infection.

[0065] 3. The present invention also relates to the use of Lactobacillus acidophilus LA-10A, which is characterized in...

Embodiment 3

[0068] Embodiment 3: strain screening / detection

[0069] The Helicobacter pylori bacterial strain that the present invention is used for testing is model strain HpSS1

[0070] 2. This patent will use the following culture medium and solution, and its preparation method is as follows:

[0071] 2.1 Improved MRS liquid medium: 10 grams of peptone, 10 grams of beef extract, 5 grams of yeast extract, 2 grams of diammonium hydrogen phosphate, 20 grams of glucose, 1.0 mL of Tween-80, 5 grams of sodium acetate, 2 grams of dipotassium hydrogen phosphate , 0.58 grams of magnesium sulfate, 0.25 grams of manganese sulfate, 1000 mL of distilled water, pH 6.2 to 6.5; high temperature sterilization before use.

[0072] 2.2 Improved MRS solid medium: Add 18 grams of agar on the basis of the improved MRS liquid medium.

[0073] 2.3 Artificial gastric juice: Dissolve the filtered pepsin with sterile 0.5% NaCl solution to make the final concentration of pepsin 3g / L, and adjust the pH to 2.0, 2...

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Abstract

The invention provides a method of screening a bacterial strain capable of inhibiting helicobacter pylori. The method includes a way that qualitative and quantitative assessment can be directly conducted on screened bacterial strains if the screened bacterial strains can inhibit or eliminate the colonization of the helicobacter pylori on gastric mucosal cells by utilizing DNA fluorescent labels and flow cytometry. According to the invention, the following points are considered when conducting screening model tests in vitro: (1) viability in an extremely acidic stomach environment; (2) inhibition ability of the bacterial strains itself or metabolites of the bacterial strains to HP reproduction in vitro; and (3) ability of inhibiting or relieving the colonization of the helicobacter pylori strains on gastric mucosa. According to the invention, developing the method of directly assessing in vitro if the HP colonization can be inhibited is of great significance to the practical applicationof the bacterial strain provided by the invention. According to the invention, lactobacillus acidophilus LA-10A capable of inhibiting the helicobacter pylori is screened out. According to a test, thelactobacillus acidophilus LA-10A can relieve or prevent the helicobacter pylori infection. The lactobacillus acidophilus LA-10A can be used to prepare functional food or pharmaceutical compositions.

Description

technical field [0001] The invention belongs to the field of microorganisms, in particular to Lactobacillus acidophilus. Background technique [0002] Helicobacter pylori (HP) is the main cause of chronic gastritis, duodenitis and gastric ulcer, and is also a major risk factor for gastric cancer and gastric lymphoma. More than 50% of the world's population is infected with HP[1], but only about 15% of the population will cause disease. The reason for this result is not only related to the genetic susceptibility and environmental factors of the host itself, but also related to the virulence of the infected strain [2]. With the development of modern biomolecular technology, people have discovered HP strains with different virulence, and the traditional triple or quadruple therapy (PPI plus 2 or 3 antibiotics) has different eradication rates for HP strains with different virulence, Eradication may fail the first time in 10%-25% of patients [3]. The failure of eradication not...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/06C12Q1/04C12N1/20A61K35/747A61P31/04A23C9/123A23C11/10A23L2/38A23L33/135C12R1/23A23L11/50A23L11/65
CPCC12Q1/06C12Q1/04A61K35/747A61P31/04A23C9/1234A23C11/106A23L2/382A23L33/135G01N2333/335A23V2002/00C12R2001/23C12N1/205A23V2400/113A23V2200/32A23V2200/3204
Inventor 喻扬郁雪平吴婷婷
Owner THANKCOME BIOLOGICAL SCI & TECH CO LTD
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