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Composite additive for resisting macrobrachium rosenbergii larva enterobacterium diseases and preparation method and applications thereof

A technology of anti-Macrobrachium rosenbergii and compound additives, which can be used in the forming or processing of antibodies, antibacterial drugs, animal feed, etc., can solve problems such as difficult control of Enterobacter cloacae, and achieve good disease resistance, high nutritional value, Immunity-boosting effect

Inactive Publication Date: 2019-01-15
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, it is difficult to control the occurrence and prevalence of diseases caused by Enterobacter cloacae in the production of Macrobrachium rosenbergii seedlings. There is a lack of a disease-resistant and immune-enhancing additive that is easy to use and suitable for Macrobrachium rosenbergii larvae.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1, a kind of composite additive of anti-Macrobrach rosenbergii larva enterobacteriasis, it is made up of following three kinds of raw materials of weight ratio,

[0026] (1) Egg yolk antibody against Enterobacteriaceae rosenbergii larvae 1;

[0027] (2) Three flavors of Chinese herbal medicine extract 1;

[0028] (3) β-cyclodextrin; β-cyclodextrin accounts for 5% of the total weight of the compound additive.

[0029] The preparation method of the described anti-Macrobrach rosenbergii larva Enterobacteriaceae yolk antibody liquid is as follows:

[0030] ① The main pathogen Enterobacter cloacae ( Enterobacter cloacae ) XL3-1 strains were inoculated on ordinary nutrient broth medium, cultured on a shaker at 28°C for 24 hours, added formaldehyde with a final volume concentration of 0.3% at 28°C for 48 hours, and centrifuged to obtain inactivated bacteria;

[0031] ② Soak Astragalus membranaceus in distilled water for 30 minutes, decoct, filter, concentrate to...

Embodiment 2

[0035] Embodiment 2, a kind of composite additive of anti-Macrobrach rosenbergii larva enterobacteriasis, it is made up of following three kinds of raw materials of weight ratio,

[0036] (1) Egg yolk antibody against Enterobacteriaceae rosenbergii larvae 1;

[0037] (2) Three flavors of Chinese herbal medicine extract 3;

[0038] (3) β-cyclodextrin; β-cyclodextrin accounts for 8% of the total weight of the compound additive;

[0039] The preparation method of the described anti-Macrobrach rosenbergii larva Enterobacteriaceae yolk antibody liquid is as follows:

[0040] ① The main pathogen Enterobacter cloacae ( Enterobacter cloacae ) XL3-1 strains were inoculated on ordinary nutrient broth medium, cultured on a shaker at 30°C for 24 hours, added formaldehyde with a final volume concentration of 0.3% at 28°C for 48 hours, and centrifuged to obtain inactivated bacteria;

[0041] ② Soak Astragalus membranaceus in distilled water for 60 minutes, decoct, filter, concentrate unti...

Embodiment 3

[0045] Embodiment 3, a kind of compound additive of anti-Macrobrach rosenbergii larva enterobacteriasis, it is made up of following three kinds of raw materials of weight ratio,

[0046] (1) Egg yolk antibody against Enterobacteriaceae rosenbergii larvae 1;

[0047] (2) Three flavors of Chinese herbal medicine extract 2;

[0048] (3) β-cyclodextrin; β-cyclodextrin accounts for 6% of the total weight of the compound additive;

[0049] The preparation method of the described anti-Macrobrach rosenbergii larva Enterobacteriaceae yolk antibody liquid is as follows:

[0050] ① The main pathogen Enterobacter cloacae ( Enterobacter cloacae ) XL3-1 strains were inoculated on ordinary nutrient broth medium, cultured on a shaker at 29°C for 24 hours, added formaldehyde with a final volume concentration of 0.3% at 28°C for 48 hours, and centrifuged to obtain inactivated bacteria;

[0051] ② Soak Astragalus membranaceus in distilled water for 45 minutes, decoct, filter, concentrate unt...

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PUM

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Abstract

The invention relates to a composite additive for resisting macrobrachium rosenbergii larva enterobacterium diseases. The additive is composed of an egg yolk solution for resisting macrobrachium rosenbergii larva enterobacterium diseases, an extracting liquid made from 3 Chinese herbal medicines, and beta-cyclodextrin. The egg yolk solution for resisting macrobrachium rosenbergii larva enterobacterium diseases is mainly used for reducing or protecting macrobrachium rosenbergii larvae from the infection of enterobacter cloacae; in the extracting liquid made from 3 Chinese herbal medicines, rhubarb rhizomes can inhibit the pathogenic enterobacter cloacae and have the effect of reducing or protecting the macrobrachium rosenbergii larvae from the infection of enterobacter cloacae, glossy privet fruits have the function of enhancing immunity, and hay has detoxification; and the beta-cyclodextrin mainly play a role of adhesion and sustained release on egg yolk antibody and a Chinese herbal medicine extracting liquid mixture. The additive can prevent and treat diseases caused by the pathogenic enterobacter cloacae during the cultivation of macrobrachium rosenbergii larvae, improve the disease resistance of the larvae, increase the macrobrachium rosenbergii seed yield, and reduce the use of antibiotics.

Description

technical field [0001] The invention relates to an anti-disease additive for aquaculture, in particular to a compound additive of Chinese herbal medicine and yolk antibody used for resisting enterobacteriasis of giant prawn larvae. Dual action with disease resistance. The invention also relates to a preparation method of the composite additive. Background technique [0002] Macrobrachium rosenbergii ( Macrobrachium rosenbergii ) is native to the tropical and subtropical waters of the Indian Ocean-Pacific Ocean. It is one of the largest freshwater shrimps in the world. It has the advantages of fast growth, large individual, wide appetite, easy domestication, strong adaptability, and short production cycle. Since it was introduced into my country by the Chinese Academy of Agricultural Sciences from Japan in 1976, and after the success of artificial breeding in 1977, after more than ten years of breeding promotion, the Macrobrachium rosenbergii nursery industry and breeding i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/80A23K10/30A01K61/59A23K40/10A61K39/40A61K36/708A61K36/638A61K36/484A61P31/04
CPCA61K36/484A61K36/638A61K36/708A61K39/40A23K10/30A23K40/10A23K50/80A01K61/59A61P31/04A61K2300/00Y02A40/81Y02A40/818
Inventor 张晓君高晓建杨辉刘晓丹李席席陈楠苗珍
Owner YANGZHOU UNIV
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