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Mulberry leaf disinfecting medicine composition, preparation method and application thereof

A composition and mulberry leaf technology, applied in the field of mulberry leaf disinfection medicinal compositions, can solve the problems of strong corrosiveness of foliar surface disinfectant, easily destroy mulberry leaf quality, reduce the taste of mulberry leaves, etc., achieve good application effect, reduce Cost of medication, simple effect of application method

Inactive Publication Date: 2015-12-09
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the foliar disinfectants currently used in production are mainly bleaching powder, bleaching powder essence and strong chlorine essence. During the process of spraying or cleaning and disinfecting mulberry leaves, such foliage disinfectants are highly corrosive and easy to damage mulberry leaves. quality, reduce the taste of mulberry leaves, and affect the health of silkworm farmers and pollute the environment (Yang Qiong, 2009)

Method used

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  • Mulberry leaf disinfecting medicine composition, preparation method and application thereof
  • Mulberry leaf disinfecting medicine composition, preparation method and application thereof
  • Mulberry leaf disinfecting medicine composition, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1 The killing test of pharmaceutical composition of the present invention to silkworm bacteriosis pathogenic bacteria, Beauveria bassiana conidia

[0028] Use the suspension quantitative method to kill the pathogenic bacteria of bacterial diseases, the specific steps are as follows:

[0029] 1. prepare the pharmaceutical composition containing the dibromohydantoin of different concentration, for avoiding repeating, present embodiment is respectively 1500mg / L, 1000mg / L, 500mg / L, 200mg / L, 50mg / L with the concentration of dibromohydantoin The pharmaceutical composition of L is illustrated as an example. Specifically:

[0030] Dibromohydantoin concentration is the preparation method of the pharmaceutical composition of 1500mg / L:

[0031] S01. Prepare 1000 mL of solution containing trisodium phosphate with a concentration of 5 g / L;

[0032] S02. Weigh 1.5 g of dibromohydantoin, and completely dissolve the dibromohydantoin in the solution described in S1.

[00...

Embodiment 2

[0061] Embodiment 2 The killing test of pharmaceutical composition of the present invention to polyhedrosis virus, microsporidia spore of silkworm

[0062] (1) preparation dibromohydantoin concentration is respectively 1500mg / L, 1000mg / L, 500mg / L, 200mg / L, the pharmaceutical composition of 50mg / L.

[0063] (2) Abolish the large sterile test tubes used in the detoxification test, add 0.5 mL of the test suspension stored in the refrigerator at 4°C respectively, and the concentrations of the virus polyhedron suspension and the silkworm Microsporidium spore suspension are 10% respectively. 9 pcs / mL, 1×10 9 Each / mL, then add 0.5mL of organic interfering substances, mix well, place in a 20°C water bath for 5min, use a sterile pipette to draw 4.0mL of the above-mentioned pharmaceutical composition into it, mix quickly, seal and place, and mark.

[0064] (3) After each test suspension interacts with different concentrations of dibromohydantoin disinfectant for 30 minutes, draw 0.5mL ...

Embodiment 3

[0086] The assay test of chlorophyll before and after embodiment 3 medicinal composition soaks mulberry leaf

[0087] Preparation dibromohydantoin concentration is 1500mg / L, 1000mg / L, 500mg / L, 200mg / L, the pharmaceutical composition of 50mg / L, gets the mulberry leaf that picks freshly, gets five points and marks on mulberry leaf, measures its For the content of chlorophyll, soak the measured mulberry leaves in disinfectants with different concentrations of dibromohydantoin for 5min, 10min, 20min, 30min, and 60min, take out the residual disinfectant on the surface of the mulberry leaves, and measure them at the marked 5 points. The chlorophyll content of mulberry leaves was taken, and the mulberry leaves soaked in water were used as negative control group. The results are shown in Table 7.

[0088] Table 7 The content of mulberry leaf chlorophyll when the medicinal composition soaks mulberry leaves at different times

[0089]

[0090]

[0091] Note: The negative control...

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Abstract

The invention relates to a mulberry leaf disinfecting medicine composition, a preparation method and an application thereof. The medicine composition includes dibromohydantoin being 50-1500 mg / L in concentration and trisodium phosphate being 5 g / L in concentration. In the invention, by means of composition of the dibromohydantoin and the trisodium phosphate, the medicine composition for disinfecting the mulberry leaves, wherein not only the characters that the dibromohydantoin is low in corrosion and stimulation and is high in efficiency achieved, but also the beneficial effects of excellent solubility and enlargement of sterilization range of the dibromohydantoin are achieved, so that the effect of disinfecting the mulberry leaves is achieved just by low-concentration dibromohydantoin, thereby reducing drug cost. The medicine composition is simple in application method and excellent in application effects.

Description

technical field [0001] The invention relates to the technical field of silkworm pest control, and more specifically relates to a mulberry leaf disinfection pharmaceutical composition and application thereof. Background technique [0002] Planting mulberry and raising silkworms has a long history in my country, and the amount of cocoon and silk produced currently accounts for about 80% of the world. In sericulture production, the loss due to silkworm disease is more than 10%, and even reaches more than 20% in some new sericulture areas. The occurrence of silkworm disease is mostly due to the mulberry leaves contaminated by pathogenic microorganisms eaten by silkworms. Studies have found that silkworm microspore spores have a long survival period and can survive in dust, soil, and water for 7 to 8 years (Zhang Peifen et al., 2010), and wild insects are easy to pollute mulberry leaves (Zhang Bijun, 2014). How to effectively reduce and prevent the pollution of mulberry leaves ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/50A01P1/00A01P3/00
Inventor 刘吉平张苏宁
Owner SOUTH CHINA AGRI UNIV
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