10 results about "Microsporidium" patented technology

The invention discloses a method for breeding, separating and purifying nosema bombycis, which comprises the following steps: infecting 2-year-old silkworms for 6-12 hours by using nosema bombycis with the concentration of 1.0 * 10 < 5 > spores / mL to 5.0 * 10 < 5 > spores / mL, feeding the silkworms with clean mulberry leaves until the silkworms fall asleep at 4 years old, and gradually causing pebrine death when the silkworms grow at 5 years old; the method comprises the following steps: grinding dead silkworms to prepare grinding liquid, filtering the grinding liquid, and separating out filtrate enriched with nosema bombycis; centrifuging the filtrate to obtain a precipitate which comprises grey silkworm body tissues on the upper layer and white nosema bombycis on the lower layer, shaking the centrifuge tube by using a shaking table to release the grey silkworm body tissues to supernate, pouring out the supernate, and retaining the white nosema bombycis at the bottom of the centrifuge tube. According to the method, the nosema bombycis is bred, separated and purified according to the growth rules and characteristics of the bombyx mori and the nosema bombycis, a high-quality sample can be efficiently obtained, and the nosema bombycis is high in content and few in impurity.

The invention provides application of vitamin B2 in preparation of a product for inhibiting or killing microsporidia, and belongs to the technical field of cultivation. The invention provides application of vitamin B2 (VB2) in preparation of a product for inhibiting or killing microsporidia. The VB2 can enable mature spores of the microsporidia to lose activity, so that the infection of the microsporidia is inhibited. From the perspective of safety, a proper amount of VB2 is harmless to human and animals, and can be used as a nutritional additive for animals. Moreover, the VB2 is used as a water-soluble vitamin necessary for organisms, the metabolic pathway of the VB2 is thoroughly researched, and the VB2 can participate in an oxidation-reduction reaction through a flavoenzyme system (such as FAD / FMN) and is naturally discharged out of a body without a residual risk. In addition, the VB2 keeps broad spectrum when inhibiting microsporidia, and is non-toxic to bred animals at the same time.

This invention relates to a triple nano-PCR detection method for silkworm microsporidia, Beauveria bassiana, and nucleopolyhedrovirus, belonging to the field of molecular diagnostic technology. Addressing the technical problem of existing silkworm disease diagnostic methods' inability to simultaneously, efficiently, and sensitively identify these three commonly mixed infection pathogens, this invention provides an integrated solution. The key technical points include extracting DNA from the sample to be tested, using this DNA as a template, performing nano-PCR amplification in the same reaction system using a specific primer set, and subsequently detecting the amplification products by agarose gel electrophoresis. The infection status of silkworm microsporidia, Beauveria bassiana, and nucleopolyhedrovirus is determined based on the specific bands at 733 bp, 230 bp, and 860 bp, respectively. This method is mainly used for the rapid, simultaneous diagnosis and identification of mixed infections of microsporidia, Beauveria bassiana, and hemorrhagic septicemia during silkworm rearing.

The present invention relates to the technical field of Microsporidia cryopreservation. Provided is a cryoprotectant for Enterocytozoon hepatopenaei. The cryoprotectant is mainly prepared from glycerol, dimethyl sulfoxide, and propylene glycol, with PBS buffer as a base solution. A volume percentage ratio of the raw materials dimethyl sulfoxide, glycerol, propylene glycol and PBS buffer is 10%:10%:10%:70%. The raw materials of the cryoprotectant are preferred, such that the obtained cryoprotectant achieves the optimal effect in indicators such as the mortality rate of Enterocytozoon hepatopenaei, the infection load in healthy shrimps, and the influence on polar tube protein 2 (EHP-ptp2) and heat shock protein 70 (hsp70). The present invention lays the foundation for achieving indoor preservation of Enterocytozoon hepatopenaei. The present invention solves the technical problem in the prior art that no cryoprotectant or preservation technique suitable for Enterocytozoon hepatopenaei is currently available.

The application is designed and screened according to the specific amplification primer of the conserved sequence of Chinese mitten crab microsporidium, the specific amplification primer screened is modified with a marker, and then the MIRA reaction condition is optimized; the MIRA detection technology has obvious advantages such as no need for thermal cycling, short detection time, simple operation, high sensitivity, simple equipment configuration and the like; compared with ordinary PCR detection, the MIRA detection technology of Chinese mitten crab microsporidium established in the experiment can complete the reaction at 37 DEG C in only 25 min, the minimum detection limit is 1 copies / µL, which is 10 4 copies / µL higher than that of ordinary PCR, the detection accuracy reaches 100%, and good specificity is achieved.

The invention discloses a rabbit encephalitis microsporidia nested PCR specific primer as well as a detection method and application thereof, and relates to the technical field of rabbit encephalitis microsporidia detection. The invention relates to a nested PCR (Polymerase Chain Reaction) specific primer for rabbit encephalitis microsporidia. A gene sequence of an outer forward outer primer is shown as SEQ ID NO.2; the gene sequence of the outer reverse outer primer is as shown in SEQ ID NO.3; the gene sequence of the inner side forward inner primer is as shown in SEQ ID NO. 4; and the gene sequence of the inner side forward outer and inner primers is as shown in SEQ ID NO. 5. The nested PCR primer designed by the invention is high in specificity, can only identify E.cloniculi, has no cross reaction with related species, has sensitivity improved by 100 times compared with that of conventional PCR, can detect more low-concentration positive samples, and reduces the omission ratio; the matched kit fills the market blank, and provides efficient and accurate technical support for clinic and prevention and control.

The invention provides a method, a kit and a system for detecting intestinal microsporidia based on a CRISPR-Cas12a technology, belongs to the field of nucleic acid detection, and aims to solve the problems that an existing intestinal microsporidia detection method is time-consuming, high in cost and low in sensitivity and specificity, and an existing CRISPR technology is poor in adaptability, insufficient in detection of low DNA template quantity and the like. Recombinase polymerase amplification (RPA) and nested polymerase chain reaction (PCR) are combined, a conserved genome sequence of the enterosporidia is taken as a target, high sensitivity of nucleic acid amplification and high specificity and signal amplification capability of CRISPR-Cas12a are fused, the lowest detection limit reaches 1021M, the lowest detection limit is far better than the single use effect of traditional PCR or CRISPR-Cas12a, ultra-sensitive, high-specificity, rapid and low-cost detection is realized, and the kit is suitable for clinical application. The kit is suitable for detecting enterosporidium pichia early infection or samples with low DNA template quantity.

This document discloses methods and compositions for use in various therapeutic, preventive, and control measures comprising from about 0.00001% by weight to about 100% by weight of an extract derived from sugarcane. The therapeutic, preventive, and control measures involve administering the extract to a subject, the extract comprising from about 10 catechin equivalents (CE) g / L to about 50 CE g / L of polyphenols or from about 100 CE mg / g to about 500 CE mg / g of polyphenols. These therapeutic and preventive measures include antimicrobial activity, particularly against respiratory viruses in humans, and treatment of microsporidia infections, such as EHP in shrimp and prawns. Control measures include reducing methane production in ruminants. The use of these compositions in, for example, dairy cows improves cow health by minimizing mastitis while increasing milk yield.

Particulate compositions comprising magnesium and low molecular weight chitosan and ss or dsRNA partially complementary to, binding to or at least 90% identical to mRNA targets of microsporidia and viruses pathogenic in farmed crustaceans, compositions and farmed aquatic crustaceans comprising the same, and methods for their use in treating or preventing parasitic microsporidia and viral infection in aquaculture are provided.

The invention discloses an application of a micromolecule Z1444019930 of targeted nosema bombycis aquaporin in preparation of a medicine for preventing and treating nosema bombycis, and belongs to the field of molecular biology and pathology. When the use final concentration of Z1444019930 in cells is more than 1 mu M, the Z1444019930 can inhibit the germination of nosema bombycis, and when the Z1444019930 is added to bombyx mori with the concentration of more than 1 mM along with mulberry leaves, the morbidity of the nosema bombycis can be obviously reduced. The method can be used for research, development and production of medicines for preventing and treating pebrine of silkworms, and has application and popularization values.