Culture medium for culturing lactobacillus helveticus strain with function of relieving lactose intolerance and culture method thereof

Abstract

The invention belongs to the technical field of microorganisms, and particularly relates to a culture medium for culturing a lactobacillus helveticus strain with a function of relieving lactose intolerance. A formula for a basic culture medium comprises 15 g / L of yeast peptone, 20 g / L of yeast extract, 20 g / L of lactose, 4 g / L of citric acid, 3 g / L of L-malic acid, 0.5 g / L of magnesium sulfate heptahydrate, 2 g / L of monopotassium phosphate and 5 g / L of sodium acetate, with the balance being purified water; and the pH value of the basic culture medium is 6.60. A formula for an optimized culturemedium comprises 25 g / L of yeast peptone, 35 g / L of yeast extract, 35 g / L of lactose, 4 g / L of citric acid, 3 g / L of L-malic acid, 0.5 g / L of magnesium sulfate heptahydrate, 0.5 g / L of calcium chloride, 5 g / L of sodium acetate and 2 g / L of monopotassium phosphate, with the balance being purified water; and the pH value of the optimized culture medium is 6.60. The prepared lactobacillus helveticusstrain with the function of relieving lactose intolerance has the advantages of high viable count, high enzyme activity and the like, wherein the viable count is as high as 109 cfu / mL, and the enzymeactivity reaches 0.584 U / mL.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a culture medium for cultivating Lactobacillus helveticum strains capable of alleviating lactose intolerance and a culturing method thereof. Background technique [0002] Lactose is a disaccharide unique to female mammals that is found only in milk. Lactose cannot be absorbed directly in the human body, it needs to be decomposed into glucose and galactose under the action of lactase to be absorbed. Lactase, also known as β-galactosidase, is a digestive enzyme located in the villi of the human small intestine. The enzyme can catalyze the decomposition of lactose, and also has the function of transferring galactoside. The activity and secretion of lactase in the human body are affected by primary and various secondary factors. People who lack lactose-decomposing enzymes will not be able to digest the lactose in dairy products after ingesting lactose, and then they wi...

AI Technical Summary

Problems solved by technology

Dietary lactose elimination therapy is to reduce the intake of lactose, and avoid lactose intolerance by eating lactose-free milk powder or milk, yogurt and other alternative diets; Function, and can repair small intestinal mucosal epithelial cells, promote the secretion of lactase, relieve abdominal distension, borborygmi and other clinical symptoms, such as bifidobacterium triple live bacteria tablets; lactase preparation therapy can effectively improve lactose absorption disorders without changing the lactose-containing diet, Guaranteed normal dietary nutrition intake, this therapy is widely recognized, but the production technology of domestic lactase raw materials is not yet mature, the existing production capacity cannot meet the domestic market demand, most of them need to be imported, and the cost is expensive

[0005] Lactobacillus helveticus is a relatively common lactic acid bacterium. It has been found to have probiotic properties such as preventing gastrointestinal infection, anti-hypertension, and immune regulation. It has not been found to alleviate lactose intolerance.

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