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Lactobacillus helveticus and application thereof

A Lactobacillus and bacterial agent technology, applied in the field of functional microorganisms, can solve problems such as neuronal function damage, and achieve the effects of relieving social barriers, improving manic behavior, and improving cognitive ability

Active Publication Date: 2020-07-14
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Continuous activity and proliferation of glial cells (astrocytes and microglia), and morphological changes, the release of various pro-inflammatory mediators (cytokines and chemokines) to coordinate immune responses and regulate neuronal function and connectivity, while this response can be neuroprotective (i.e., help repair damaged tissue), chronic dysregulation of CNS cytokines can lead to disruption of neuronal function through their roles in cognition and synaptic regulation, These cytokines include IL-6 and TNF-α, etc.

Method used

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  • Lactobacillus helveticus and application thereof
  • Lactobacillus helveticus and application thereof
  • Lactobacillus helveticus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] Example 1: Lactobacillus helveticus CCFM1076 has good tolerance to simulated gastrointestinal fluid

[0073] Inoculate the cryopreserved Lactobacillus helveticus CCFM1076 in mMRS medium (MRS medium + 0.05% cysteine ​​hydrochloride), culture anaerobically at 37°C for 48 hours, and subculture in mMRS medium for 2 to 3 times Finally, take 1mL of the culture solution of Lactobacillus helveticus CCFM1076, mix it with 9.0mL of pH 2.5 artificial simulated gastric juice (containing 1% pepsin, mMRS medium at pH=2.5), and culture it anaerobically at 37°C. Samples were taken at 0.5h, 1h, and 2h, and the mMRS agar medium was poured to culture the plate colonies, and the number of viable bacteria was determined and the survival rate was calculated.

[0074] The survival rate is the ratio of the logarithmic value of the number of viable bacteria at the time of sampling to the logarithmic value of the number of viable bacteria at the time of 0h in the culture fluid, expressed in %. T...

Embodiment 2

[0079] Embodiment 2: Lactobacillus helveticum CCFM1076 has no toxic and side effects to wistar rats

[0080] Lactobacillus helveticus CCFM1076 was resuspended in 10% skim milk to make a concentration of 3.0×10 8 CFU / mL bacterial suspension. Get 6 male wistar autistic rats with a body weight of about 200g. After one week of acclimatization, the bacterial suspension of this concentration was given by intragastric gavage once a day, observed for one week, and recorded death and body weight.

[0081] The results of these tests are listed in Table 3. These results indicate that feeding concentrations of 3.0×10 8 The Lactobacillus helveticus CCFM1076 of CFU / mL has no significant impact on rats, no significant change in body weight, and no death. The appearance of the rats showed no obvious pathological symptoms.

[0082] Table 3 Changes in body weight and death of mice

[0083]

[0084] Note: -: Rats did not die

Embodiment 3

[0085] Example 3: Lactobacillus helveticum CCFM1076 can significantly improve the autonomous exploration ability of autistic rats

[0086] (1) Breeding of young mice with autism

[0087] Healthy male and female wistar rats were bred and adapted to the environment for 1 week. They were caged together at 5:00 p.m. with a male-to-female ratio of 2:1. The vaginal plug was observed at 8:00 the next morning. If no vaginal plug was observed, a vaginal smear was performed. On the first day of pregnancy, the female mice were housed in separate cages after conception, and randomly divided into model group and control group. On the 12.5th day after pregnancy, intraperitoneal injection of sodium valproate (VPA) 600mg / kg (normal saline made into 250mg / mL solution) was given as the model group; Weaning.

[0088]

[0089] (2) Experimental grouping of autistic rats

[0090] Divided into control group, model group and intervention group.

[0091]

[0092]

[0093] (3) Open field t...

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Abstract

The invention discloses lactobacillus helveticus and application thereof. The lactobacillus helveticus CCFM1076 can improve the autonomous behavior and the exploration behavior caused by autism in a strange environment, relieve social disorder caused by autism, obviously improve the rigid behavior caused by autism, improve the cognitive ability of autism to new objects, improve the decline of limbcoordination ability caused by nerve injury, reduce the manic behavior caused by autism, and effectively adsorb polychlorinated biphenyl.

Description

technical field [0001] The invention belongs to the technical field of functional microorganisms, in particular to the application of Lactobacillus helveticus and Lactobacillus helveticus. Background technique [0002] Autism (Autism), also known as autism, has been recognized as a generalized neurodevelopmental disorder that frequently occurs in infants and young children, mainly manifested in social interaction impairment, communication (verbal and non-verbal) function impairment, and repetitive stereotyped behaviors. , often accompanied by a variety of clinical symptoms such as mental retardation, epilepsy or hyperactivity, usually before the age of 3. According to the latest data released by the US Centers for Disease Control and Prevention (CDC), the prevalence of ASD in 2018 was 1:59, a 15% increase from 1:68 in 2016, and the prevalence rate of men was 4 to 5 times that of women. . [0003] ASD is a highly heterogeneous neurodevelopmental disorder. Genetic factors ar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L33/135A23L11/00A23L19/00A23C9/123A61K35/747A61P25/00C12R1/225A23L11/50
CPCA23L33/135A23L19/00A23C9/1234A61K35/747A61P25/00A23V2002/00A23L11/50C12R2001/225C12N1/205A23V2400/147A23V2200/322
Inventor 陈卫王刚孔庆敏赵建新张灏
Owner JIANGNAN UNIV
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