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Lactobacillus preparation and application thereof

A technology of Lactobacillus and Lactobacillus delbrueckii, applied in the direction of Lactobacillus, application, bacteria, etc., can solve the problems of high survival rate, unfavorable microecological preparation transportation, storage and use, slow metabolism of probiotics, etc., and achieve high survival rate , improve high blood pressure, good effect of colonization

Pending Publication Date: 2021-12-07
INNER MONGOLIA YILI INDUSTRIAL GROUP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The metabolism of probiotics is slow under low temperature conditions, and the survival rate is relatively high, so most of the microecological preparations at home and abroad are required to be stored under low temperature conditions, while under normal temperature conditions, the survival rate of lactic acid bacteria generally reaches 0 in about 30 days. Conditions are also not conducive to the transportation, storage and use of probiotics

Method used

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  • Lactobacillus preparation and application thereof
  • Lactobacillus preparation and application thereof
  • Lactobacillus preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Example 1 Detection of Acid and Bile Resistance Ability of Different Bacterial Strains

[0066] 1. Select different Lactobacillus (commercial strains) to test the ability of acid resistance and bile salt resistance:

[0067] Lactobacillus delbrueckii subsp.bulgaricus L7 (referred to as GL01),

[0068] Lactobacillus paracasei 431 (referred to as GL02),

[0069] Lactobacillus plantarum LP299V (referred to as GL03),

[0070] Lactobacillus plantarum FLAV54 (referred to as GL04),

[0071] Lactobacillus rhamnosus HN001 (referred to as GL05),

[0072] Lactobacillus fermenti CECT5716 (referred to as GL06),

[0073] Lactobacillus paracasei 150DCU (marked as GL07),

[0074] Lactobacillus paracasei 10450 (referred to as GL08).

[0075] The method is as follows: after the strain is rejuvenated, insert 4% of the inoculum into the MRS liquid medium of pH 2.0 and pH 3.0 added with 0.3% bile salt, culture anaerobically at 37°C for 10 hours, and culture with sterile MRS The base ...

Embodiment 2

[0109] 1. Preparation of Lactobacillus helveticus powder:

[0110] Lactobacillus helveticus FLAV54, purchased from Danisco (China) Co., Ltd., the specific preparation steps of bacterial powder are as follows:

[0111] ① After diluting the freeze-dried bacteria with normal saline, pick a ring with an inoculation loop and streak it on the MRS solid medium, and then culture it in an anaerobic box at 38°C for 48 hours;

[0112] ② Pick a single colony and continue to streak and purify it on the MRS plate, and culture it in an anaerobic box at 38°C for 48 hours;

[0113] ③ Select a plate with good growth and colony morphology, scrape all the bacterial lawn into the first-grade MRS liquid medium with an inoculation loop, and culture it anaerobically at 38°C for 11-12 hours;

[0114] ④ Take out the primary fermentation liquid and insert it into the secondary MRS liquid medium with 5% inoculum amount, and culture it anaerobically at 38°C for 8-9 hours;

[0115] ⑤Centrifuge the second...

Embodiment 3

[0121] The preparation method of Lactobacillus helveticus bacterium powder is the same as embodiment 2, Lactobacillus helveticus GL04 bacterium powder, pregelatinized starch and magnesium stearate are formulated by following mass fraction: Lactobacillus helveticus bacterium powder 0.5%, magnesium stearate 0.5% %, the balance is pregelatinized starch. The preparation method of the capsule is as follows: mix the bacteria powder with the pregelatinized starch, then add magnesium stearate, mix well and put it into a gelatin hollow capsule, the volume of the capsule is 0.5mL, and the number of viable bacteria per capsule is about 350 million.

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Abstract

The invention relates to the technical field of probiotic preparations, in particular to a lactobacillus preparation and application thereof. According to the lactobacillus preparation provided by the invention, magnesium stearate and pregelatinized starch are used as auxiliary materials, so that the preparation can be stored at normal temperature, the survival rate of thalli can be kept high, and the lactobacillus preparation has good colonization capability in intestinal tracts. Based on the ACE inhibitory activity of lactobacillus, especially lactobacillus helveticus, the lactobacillus preparation provided by the invention can have the effect of improving hypertension, can be used as a medicine for improving hypertension or a health-care product suitable for hypertensive patients or food for special medical purposes, and can also be used as an additive of food. Experiments show that after the lactobacillus preparation provided by the invention is stored for 12 months at room temperature, the survival rate of the lactobacillus still reaches 72.28%.

Description

technical field [0001] The invention relates to the technical field of probiotic preparations, in particular to a lactobacillus preparation and its application. Background technique [0002] Hypertension is one of the most common cardiovascular diseases, and it is the most important pathogenic factor of coronary heart disease, sudden cerebral death, heart and renal failure. Angiotensin converting enzyme (Angiotensinconvertingenzyme, ACE) is the target enzyme for screening antihypertensive drugs. Through the action of ACE, angiotensin I can be converted into angiotensin II, which has the function of constricting blood vessels and boosting blood pressure; at the same time, ACE will Bradykinin with antihypertensive function is degraded, making it lose its antihypertensive effect, causing blood pressure to rise. Angiotensin inhibitors (ACEI) can inhibit the activity of ACE and play a role in lowering blood pressure. Antihypertensive drugs have certain side effects in the treat...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/04A23L33/135A61K35/747A61K47/12A61K47/36A61P9/12C12R1/225C12R1/25
CPCC12N1/20C12N1/04A23L33/135A61K35/747A61K47/12A61K47/36A61P9/12A23V2002/00A23V2400/137A23V2400/147A23V2400/143A23V2400/175A23V2400/169A23V2200/326
Inventor 刘斌吴超马国文张海斌
Owner INNER MONGOLIA YILI INDUSTRIAL GROUP CO LTD
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