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A probiotic strain for improving the health of respiratory tract as well as a composition and application of the probiotic strain

A respiratory and composition technology, applied in the field of microorganisms, can solve the problems of increasing indoor air pollution and exposure risks, and achieve the effects of promoting phagocytosis, inhibiting bacterial growth, and improving repair ability

Active Publication Date: 2020-07-21
TCI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, at present, the inhalation of PM2.5 can only be slowed down through negative methods to prevent or slow down the damage caused by PM2.5 to the human body
Among them, PM2.5 in the atmospheric environment can reduce the risk of exposure by reducing outdoor activity time and wearing personal protective equipment such as masks, etc., but PM2.5 will seep into the indoor environment through ventilation and air conditioning systems, doors, windows, and building gaps, increasing the risk of exposure. Indoor Air Pollution and Exposure Risks

Method used

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  • A probiotic strain for improving the health of respiratory tract as well as a composition and application of the probiotic strain
  • A probiotic strain for improving the health of respiratory tract as well as a composition and application of the probiotic strain
  • A probiotic strain for improving the health of respiratory tract as well as a composition and application of the probiotic strain

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Experimental program
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Effect test

Embodiment 1

[0064] Embodiment 1 The preparation method of Lactobacillus helveticum metabolites of the present invention

[0065] In an embodiment of the present invention, the frozen strain preservation tube of Lactobacillus helveticus of the present invention is cultivated in MRS (de Man, Rogosa and Sharpe, BD Difco TM In the Lactobacilli MRS Broth) culture medium, it is preferred to add 0.1 mL of activated strains to 10 mL of MRS, and after culturing at 37°C for 16-18 hours, centrifuge the culture liquid at 5000 rpm for 10-20 minutes, and collect the The supernatant is the metabolite of Lactobacillus helveticus of the present invention.

Embodiment 2

[0066] Embodiment 2 Lactobacillus helveticum metabolites of the present invention are effective in inhibiting bacterial growth

[0067] One embodiment of the present invention In order to test the effectiveness of the Lactobacillus helveticum metabolites of the present invention in inhibiting bacterial growth, use the zone of inhibition method to test its bacteriostasis effect, wherein the zone of inhibition method is also called the diffusion method, which is to use the test substance in the Diffusion in agaric gum inhibits the growth of bacteria around it to form a transparent zone, that is, the zone of inhibition, and the method of judging the antibacterial effect of the test object according to the size of the zone of inhibition is often used for the isolation and screening of antibiotic-producing bacteria . First, the Lactobacillus helveticus of the present invention was cultured in the MRS medium, and after culturing at 37° C. for 16 hours, the bacterial solution was cen...

Embodiment 3

[0069] Embodiment 3 Lactobacillus helveticus and its metabolites of the present invention are effective in repairing lung epithelial cells

[0070] In one embodiment of the present invention, human bronchial epithelial cells BEAS-2b (purchased from the American Type Culture Collection, No. CRL-9609) were used to test the Lactobacillus helveticus and its metabolites of the present invention in cell scratch repair experiments. The effect of repairing lung epithelial cells. The cells are cultured in Bronchial epithelial cell basal Medium (BEBM, purchased from Lonza, Switzerland). The cell scratch damage repair experiment is to observe the cells refilling an artificially created scratch to know the healing ability of the cells . First, place the 1.5x10 5 A BEAS-2b cell was cultured in each well of a 24-well culture dish, and cultured at 37°C for 16-18 hours to spread the cells on the bottom of the well in the culture dish, and then use a 200μL micropipette on the monolayer of ce...

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Abstract

The invention relates to the field of microorganisms, in particular to a probiotic strain for improving the health of respiratory tract as well as a composition and application of the probiotic strain. The probiotic strain for improving respiratory tract health provided by the invention is lactobacillus helveticus (numbered as DSM 33107), and the lactobacillus helveticus and metabolites thereof have the capabilities of inhibiting the growth of bacteria, promoting the repair of lung epithelial cells and promoting the phagocytosis of macrophages; the infection probability of the respiratory tract can be effectively reduced, the repairing capability of the respiratory tract is improved, PM2.5 in a human body is promoted to be removed, and foreign matters in a respiratory tract can be removed.The metabolite of the lactobacillus helveticus disclosed by the invention contains an effective component of 1, 2, 3, 4-tetrahydro-beta-carboline-3-carboxylic acid.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a probiotic strain for improving respiratory health, its composition and use. Background technique [0002] The development of industrial activities and the heavy demand for energy in modern life, but the process of obtaining energy has caused serious air pollution. According to the results of Harvard University's research on air pollution in Asia and Southeast Asia, coal-fired power plants not only directly emit PM2.5, but also emit sulfur compounds (SO 2 ), nitrogen oxides (NOX), soot and dust, etc. will indirectly promote the formation of PM2.5. PM2.5 particles are very small and are respirable particles, which can easily break through the respiratory defense mechanisms such as nasal villi and tracheal cilium mucus, enter the bronchi and alveoli, and cause serious health effects, including respiratory diseases, chronic bronchial Inflammation, lung cancer and cardiovascular dise...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/747A61P11/00A61P31/04A61P37/04A61K31/437C12R1/01
CPCA61K35/747A61P11/00A61P31/04A61P37/04A61K31/437C12R2001/225C12N1/205C12R2001/01
Inventor 林咏翔
Owner TCI CO LTD
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