Lactobacillus helveticus ZJUIDS12 capable of improving alcoholic liver disease and application of lactobacillus helveticus ZJUIDS12

A technology of lactobacillus and bacteria powder, applied in the field of food microorganisms, can solve the problem of no safe and effective treatment of ALD, and achieve the effects of inhibiting Helicobacter pylori ability, improving alcoholic liver disease, and strong antioxidant ability

Active Publication Date: 2022-04-22
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, ALD has become a public health problem of global concern, but so far there is no safe and effective clinical drug for the treatment of ALD.

Method used

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  • Lactobacillus helveticus ZJUIDS12 capable of improving alcoholic liver disease and application of lactobacillus helveticus ZJUIDS12
  • Lactobacillus helveticus ZJUIDS12 capable of improving alcoholic liver disease and application of lactobacillus helveticus ZJUIDS12
  • Lactobacillus helveticus ZJUIDS12 capable of improving alcoholic liver disease and application of lactobacillus helveticus ZJUIDS12

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1, screening and identification of Lactobacillus helveticus ZJUIDS12:

[0030] 1. Screening of Lactobacillus helveticus ZJUIDS12

[0031] 1.1 Sample source

[0032] The bacterial strain used in the present invention is isolated from sour cream products made by Mongolian herdsmen in Inner Mongolia, and 20 samples are collected in total.

[0033] 1.2 Isolation and purification of strains

[0034] About 5 g of fresh samples were collected with sterile tubes and immediately sent to the laboratory for strain isolation. Take 1 g of the sample and put it into 9 mL of MRS broth medium, vortex and mix it, and enrich and culture it at 37°C for 48 hours; then absorb 1 mL of the enriched solution in an ultra-clean bench, and perform a ten-fold gradient dilution with sterile normal saline, Pick 10 -6 、10 -7 、10 -8 For three dilution gradients, 100 μL of bacterial solution was taken from each gradient and spread on MRS agar medium, and cultured at 37°C for 48h. Afte...

Embodiment 2

[0047] Embodiment 2, Lactobacillus helveticus ZJUIDS12 improves alcoholic liver disease:

[0048] 1. Experimental animals: 24 C57BL / 6 male mice were purchased from Shanghai Slack Experimental Animal Center, company license number: SCXK (Shanghai) 2017-0005, and were kept in SPF environment in the Experimental Animal Center of Zhejiang University of Traditional Chinese Medicine.

[0049] Reagent: Lieber-DeCarli alcohol liquid model feed (product code TP-4030B, Nantong Trophy Feed Technology Co., Ltd.); Lieber-DeCarli contrast liquid feed (product code TP-4030D, Nantong Trophy Feed Technology Co., Ltd.); bile Alkali, vitamins (Nantong Trophy Feed Technology Co., Ltd.). Absolute ethanol (CAS-NO: 64-17-5, lichrosolv), ALT kit (Cat. No.: C009-2 Nanjing Jiancheng Bioengineering Research Institute), AST kit (Cat. No.: C010-2 Nanjing Jiancheng Bioengineering Research Institute) Free Fatty Acid Kit (Cat. No.: A042-2-1 Nanjing Jiancheng Institute of Bioengineering), Tissue Triglyceride...

Embodiment 3

[0110] Embodiment 3, inactivated Lactobacillus helveticus ZJUIDS12 thallus improves alcoholic liver disease:

[0111] 1. Experimental animals: 32 C57BL / 6 male mice were purchased from Shanghai Slack Experimental Animal Center, company license number: SCXK (Shanghai) 2017-0005, and were kept in SPF environment in the Experimental Animal Center of Zhejiang University of Traditional Chinese Medicine.

[0112] Reagent: Lieber-DeCarli alcohol liquid model feed (product code TP-4030B, Nantong Trophy Feed Technology Co., Ltd.); Lieber-DeCarli contrast liquid feed (product code TP-4030BC, Nantong Trophy Feed Technology Co., Ltd.); bile Alkali, vitamins (Nantong Trophy Feed Technology Co., Ltd.). Absolute ethanol (CAS-NO: 64-17-5, lichrosolv), ALT kit (Cat. No.: C009-2 Nanjing Jiancheng Bioengineering Research Institute), AST kit (Cat. No.: C010-2 Nanjing Jiancheng Bioengineering Research Institute) Free fatty acid kit (article number: A042-2-1 Nanjing Jiancheng Institute of Bioengine...

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Abstract

The invention relates to the technical field of food microorganisms, in particular to lactobacillus helveticus ZJUIDS12 capable of improving alcoholic liver injury and application of the lactobacillus helveticus ZJUIDS12. The invention discloses lactobacillus helveticus ZJUIDS12, and the preservation number of the lactobacillus helveticus ZJUIDS12 is CGMCC (China General Microbiological Culture Collection Center) NO.23997. The lactobacillus helveticus ZJUIDS12 can be used for preparing the lactobacillus helveticus. The invention also discloses an application of the lactobacillus helveticus ZJUIDS12 in the preparation of a product for protecting the liver injury at the same time, and the lactobacillus helveticus ZJUIDS12 can be used for preparing the product for protecting the liver injury at the same time.

Description

technical field [0001] The invention relates to the technical field of food microbes, in particular to Lactobacillus helveticum ZJUIDS12 capable of improving alcoholic liver damage and its application. Background technique [0002] Current studies have reported that alcohol is a common cause of chronic liver disease in most industrialized countries, and the liver is the main organ that metabolizes alcohol. Therefore, the liver has long been considered the main target organ of alcohol damage. Therefore, alcoholic liver disease (alcoholic liver disease, ALD) has gradually become a chronic liver disease that is widely prevalent in the world. In the early stages of the ALD disease process, lipids will enter the liver cells under the induction of ethanol and gradually accumulate to form fatty liver. If you continue to consume alcohol at this time, it may further develop into liver fibrosis or even cirrhosis. At present, ALD has become a public health problem of global concern, b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/747A61P1/16C12R1/225
CPCA61K35/747A61P1/16C12N1/20A61K2035/115C12N1/205
Inventor 任大喜丁秦超曹菲薇李松涛韩强
Owner ZHEJIANG UNIV
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