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High-density culture method of lactobacillus helveticus, and preparation and application of high-activity bacterial powder

A technology of lactobacillus and high vigor, which is applied in the preparation of high vigor powder and high-density culture of Lactobacillus helveticus, which can solve the problems of long culture period and low vigor of fermentation broth, and achieve reduced fermentation cost and excellent adhesion Effects of shortening of performance and culture time

Active Publication Date: 2020-12-25
HANGZHOU WAHAHA TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Its shortcoming is that the fermented liquid bacterial cell vigor that is cultivated is low, and the culture period is long

Method used

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  • High-density culture method of lactobacillus helveticus, and preparation and application of high-activity bacterial powder
  • High-density culture method of lactobacillus helveticus, and preparation and application of high-activity bacterial powder
  • High-density culture method of lactobacillus helveticus, and preparation and application of high-activity bacterial powder

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preparation example Construction

[0054] 4. Preparation of freeze-dried bacteria powder: The collection method of bacteria slime, freeze-drying protective agent and freeze-drying process were optimized.

[0055] a. Sludge collection: adjust the pH of the fermentation broth obtained in the above step 3 to 6.0-7.0 with ammonia water, centrifuge at room temperature at 4000 rpm for 10-20 minutes, collect the sludge, and adjust the water content of the sludge to 70%-80%.

[0056] b. Addition of protective agent:

[0057] Based on the general protective agent formula in the laboratory (including lactose, skim milk, glycerin or sodium glutamate), the types and concentrations of different sugars, different concentrations of skim milk, different concentrations of glycerin and different concentrations of sodium glutamate, the addition of ascorbic acid , calcium chloride, yeast extract or glutathione concentration, and the pH of the freeze-drying protectant on the number of viable bacteria in the freeze-dried bacterial p...

Embodiment 1

[0073] Dissolve 204g of whey powder, 28g of whey protein concentrate, 144g of soytone, 68g of concentrated tomato juice, 60g of yeast powder, 1.68g of manganese sulfate, 3.48g of magnesium sulfate, 18g of dipotassium hydrogen phosphate and 48g of sodium citrate in 5L of warm pure water , after cutting for 20-30 minutes, set the volume to 6L, and pour it into a 10L fermenter. Autoclave at 121°C for 10 minutes. After cooling to room temperature, the seed solution of Lactobacillus helveticus obtained in Example 1b was connected to a 10L fermenter at a 5% (v / v) inoculation amount. Fermentation temperature 40°C, CO2 2 , the stirring speed is controlled at 150rpm, the pH is controlled at 5.2 during fermentation, and the fermentation time is 6h. The number of live bacteria in the live bacteria fermented liquid obtained at this time was 5.50×109 cfu / mL. Adjust the pH of the fermentation broth to 6.8 with ammonia water, centrifuge at room temperature at 4000 rpm for 15 minutes, coll...

Embodiment 2

[0075] Dissolve 60g of whey powder, 28g of whey protein concentrate, 144g of soybean peptone, 68g of concentrated tomato juice, 60g of yeast powder, 1.68g of manganese sulfate, 3.48g of magnesium sulfate, 18g of dipotassium hydrogen phosphate and 48g of sodium citrate in 5L of warm pure water , after cutting for 20-30 minutes, set the volume to 6L, and pour it into a 10L fermenter. Autoclave at 121°C for 10 minutes. After cooling to room temperature, the seed solution of Lactobacillus helveticus obtained in Example 1b was connected to a 10L fermenter at a 5% (v / v) inoculation amount. Fermentation temperature 40°C, CO2 2 , the stirring speed is controlled at 150rpm, the pH is controlled at 5.2 during fermentation, and the fermentation time is 6h. The number of live bacteria in the live bacteria fermentation broth obtained at this time was 4.65×10 9 cfu / mL. The difference from Example 1 is that the addition amount of whey powder in the culture medium of this embodiment is re...

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Abstract

The invention relates to the technical field of microbial culture and application, and discloses a high-density culture method of lactobacillus helveticus, and preparation and application of high-activity bacterial powder, aiming at the problems of low activity and long culture period of lactic acid bacteria in the prior art. The high-density culture method comprises the following steps of (1) inoculating a lactobacillus helveticus strain into a seed culture medium for activation, performing culturing to obtain a seed solution; and (2) inoculating the seed solution into a lactobacillus helveticus high-density fermentation culture medium, and carrying out fermentation culture at 35-43 DEG C under an anaerobic stirring condition to obtain the viable bacteria fermentation liquor after the fermentation is finished. The fermentation liquor obtained by the method is applied to preparation of high-activity lactobacillus helveticus powder. A high-density whey proliferation culture medium is provided, and lactobacillus helveticus WHH2580 is subjected to high-density culture, so that a large number of thalli can be obtained. By adopting an optimized freeze-drying protective agent, the survival rate of thalli is greatly increased, the high-activity lactobacillus helveticus powder is prepared, and the preservation period of the freeze-dried powder is prolonged.

Description

technical field [0001] The invention relates to the technical field of microorganism cultivation and application, in particular to a high-density cultivation method of Lactobacillus helveticus, preparation and application of high-viability bacterial powder. Background technique [0002] Probiotics are a kind of active microorganisms that are beneficial to the host. They are a general term for active beneficial microorganisms that colonize the human intestinal tract and reproductive system and can produce definite health effects, thereby improving the host's micro-ecological balance and exerting a beneficial effect on the intestinal tract. . Most of the probiotics that have been discovered can be divided into three categories, namely lactobacilli, bifidobacteria and gram-positive cocci. Lactobacillus helveticus is a type of Lactobacillus, which is in the list of probiotic strains approved by the Ministry of Health of my country that can be used in food. This strain was firs...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/04C12R1/225
CPCC12N1/20C12N1/04
Inventor 陈丽娥陈苏陈彩玲任学良翁璐溦郑志瑶李言郡林国栋陈作国赵越李理
Owner HANGZHOU WAHAHA TECH
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