Bacillus subtilis preparation capable of being applicable to intestinal tract fixed implantation and improving digestion rate and immunity and preparation method thereof

A technology of Bacillus subtilis and digestibility, which is applied in the technical field of feed additives for poultry and livestock breeding, can solve problems such as excessive use of antibiotics, antibiotic residues in poultry and livestock products, hidden food safety hazards, etc., and achieve improved antioxidant capacity, good acid resistance and The effect of bile salt and good antibacterial activity

Inactive Publication Date: 2019-03-26
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Many diseases often occur in poultry and livestock production, especially in high-density breeding in large-scale breeding areas. In order to prevent the spread of infectious diseases, antibiotics and vaccinations are often used in large quantities to prevent the occurrence of diseases. Laying hens have been vaccinated with more than a dozen vaccines since the chicks, which will bring serious antibiotic residues in poultry and livestock products, which will bring serious hidden dangers to our food safety

Method used

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  • Bacillus subtilis preparation capable of being applicable to intestinal tract fixed implantation and improving digestion rate and immunity and preparation method thereof
  • Bacillus subtilis preparation capable of being applicable to intestinal tract fixed implantation and improving digestion rate and immunity and preparation method thereof
  • Bacillus subtilis preparation capable of being applicable to intestinal tract fixed implantation and improving digestion rate and immunity and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: the separation and identification of this Bacillus subtilis:

[0024] Collect bacterial cells: Take 1 ml of the mixed bacterial liquid from fermented plants, centrifuge at 3500 r / min for 10 min in a centrifuge, collect the supernatant in another clean centrifuge tube, discard the precipitate; put the collected supernatant in Centrifuge at 10,000 r / min for 10 min in a high-speed centrifuge, collect the precipitated bacteria, and discard the supernatant.

[0025] Collect spores: Add 1 ml of sterile water to the centrifuge tube used to collect bacteria, mix well on a vortex shaker, place in a constant temperature water bath at 80°C for 15 minutes, kill vegetative cells and other bacterial cells, and collect spores.

[0026] Spore recovery: Inoculate an appropriate amount of spore collection liquid into LB liquid medium in a clean bench, place on a constant temperature shaker at 37 °C and 180 r / min for enrichment and culture for 24-36 hours, and then take 1 ml...

Embodiment 2

[0043] Example 2: Cultivation and enrichment of Bacillus subtilis: The purified strain was added to LB liquid medium (per liter of water: tryptone 10g, yeast extract 5g, NaCl 10g pH 7.0) at a ratio of 1:100. 37°C, 180 r / min placed on a constant temperature shaker for enrichment culture for 24-36 h.

Embodiment 3

[0044] Example 3: Antioxidant activity detection of Bacillus subtilis on Drosophila

[0045] 1. Test materials: w1118 wild-type Drosophila; the Bacillus subtilis strain of the present invention, the composite bacterial solution of Bacillus pumilus (513-A) and Bacillus gobiensis (513-O) preserved in our laboratory.

[0046] 2. Preparation of Drosophila basal medium: Liquid A: Weigh 10g of agar powder, 70g of edible white sugar, add to 500ml of distilled water and boil. Liquid B: Weigh 85g of corn flour, add it to 500ml of distilled water and boil.

[0047] Slowly pour liquid B into liquid A, stir while heating, cool slightly after forming a paste, add 18g yeast powder to the mixture, then stir evenly, then add 5ml propionic acid, mix evenly, divide into sterilized containers In Drosophila culture tubes (3×10 cm), add 5 ml of medium to each tube, seal with a cotton plug, and store at 4°C for future use.

[0048] 3. Preparation of Drosophila culture medium containing Bacillus s...

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Abstract

The invention belongs to the technical field of poultry and livestock culture feed additives, and provides a bacillus subtilis preparation capable of being applicable to intestinal tract fixed implantation and improving digestion rate and immunity. The bacillus subtilis preparation can be fixedly implemented into the animal intestinal tract to improve the microbial flora balance in the host intestinal tract and to achieve the antibacterial and disease prevention effects, can also generate various amylase, cellulase and protease in the animal intestinal tract to improve the animal feed utilization rate. The bacillus subtilis mud and an adsorption agent are mixed according to the mass ratio of 1:9; spray drying is performed to obtain bacterial powder; the adsorption agent uses the total weight of the adsorption agent as the calculation reference, and is prepared by proportionally mixing soluble starch, jerusalem artichoke powder, saccharose and galactose. The feed utilization rate can beimproved; the antibacterial effects are achieved on the intestinal tract pathogenic bacteria; high acid resistance and bile salt tolerance capability are realized; the stable fixed implementation isrealized in the intestinal tract; a novel breeding technology is provided for poultry and livestock industry. The referential breeding technology is provided for other plant varieties difficult to perform normal sexual propagation.

Description

technical field [0001] The invention belongs to the technical field of feed additives for poultry and livestock breeding, and in particular relates to a Bacillus subtilis preparation suitable for intestinal colonization, improving digestibility and immunity and a preparation method thereof. The prepared microbial feed additive can be colonized in animal intestines , improve the balance of microbial flora in the host's intestines, have the functions of antibacterial, disease prevention and disease resistance, and can produce a variety of amylases, cellulases and proteases in the animal intestines to improve the utilization rate of animal feed. Background technique [0002] Many diseases often occur in poultry and livestock production, especially in high-density breeding in large-scale breeding areas. In order to prevent the spread of infectious diseases, antibiotics and vaccinations are often used in large quantities to prevent the occurrence of diseases. Laying hens have bee...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K10/18A23K50/75A23K50/90C12R1/125
CPCA23K10/18A23K50/75A23K50/90C12N1/20
Inventor 王景雪陈雅茜
Owner SHANXI UNIV
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