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Lactobacillus casei with antihypertensive effect and application thereof

A Lactobacillus casei and blood pressure-lowering technology, applied in the field of functional bacteria, can solve the problems of low ACE inhibition rate, etc., and achieve the effects of high ACE inhibitory activity, strong inhibitory effect, and good acid resistance and bile salt resistance.

Active Publication Date: 2022-07-15
GUANGXI ZHUANG AUTONOMOUS REGION BUFFALO INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, some strains that have been screened have a low ACE inhibition rate

Method used

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  • Lactobacillus casei with antihypertensive effect and application thereof
  • Lactobacillus casei with antihypertensive effect and application thereof
  • Lactobacillus casei with antihypertensive effect and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The Lactobacillus casei SLK30 provided by the present invention is derived from a fermented feed sample. The strain is sequenced and analyzed, and the sequence obtained by sequencing is compared in the EzBioCloud Database. The result shows that the strain is Lactobacillus casei, and it is named Cheese Lactobacillus casei SLK30. Its microbial morphological characteristics are: round, milky white and translucent, smooth surface, convex, and neat edge. Microscopic observation appears in pairs or chains, belonging to Gram-positive strains. Bacterial form such as figure 1 shown.

[0031] Hemolytic capacity assay

[0032] Lactobacillus casei SLK30 was inoculated into sterilized MRS broth medium at 2% inoculation amount, cultured at 37° C. for 24 h, activated to 2 generations, and the strain vitality was restored to normal, ready for use.

[0033] Lactobacillus casei SLK30 was inoculated into a blood agar plate containing 5% sterile defibrillated sheep blood with a one-tim...

Embodiment 2

[0035] GABA-producing ability assay

[0036] Add 1% L-sodium glutamate to the MRS broth medium, sterilize by autoclaving at 121°C for 15 min, and inoculate the Lactobacillus casei SLK30 activated for 2 generations in Example 1 at an inoculum of 3% in the MRS medium. , after standing at 37°C for 24h, centrifugation (5200r / min, 20min), collecting the fermentation supernatant and storing it in a -40°C refrigerator for later use.

[0037] GABA standard dilution sample concentration: 0.1g / L, 0.2g / L, 0.4g / L, 0.6g / L, 0.8g / L; take 200μL of GABA standard dilution samples respectively, add 600μL of OPA derivatization reagent, and add 800μL after mixing KH 2 PO 4 Buffer, vortex mixed, filter through organic membrane, and wait for injection.

[0038] Chromatographic column: Agilent C-18 column; mobile phase A (15mmol / L CH 3 COOH-CH 3 COONa buffer): mobile phase B (methanol)=55:45; 0.8 mL / min; 30° C.; 334 nm; automatic injection; the injection volume was 20 μL.

[0039] Obtain the ch...

Embodiment 3

[0042] Ability to inhibit ACE rate

[0043] The 2-generation activated Lactobacillus casei SLK30 in Example 1 was inoculated into sterilized 12% skimmed milk powder according to the inoculum amount of 3%, and fermented at 42° C. for 34 hours to obtain fermented milk. The fermented milk was centrifuged at 4500 g for 10 min. The supernatant was taken, and the pH was adjusted to 7.5 with 1.0 mol / L NaOH, and the inhibitory activity of Lactobacillus casei SLK30 on ACE was determined by ACE kit. The assay method of ACE inhibitory activity was determined according to the method in the instructions of the ACE kit. The results showed that the inhibitory rate of Lactobacillus casei SLK30 on ACE was 89.99%, and the inhibitory activity on ACE was high.

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Abstract

The invention provides lactobacillus casei with an effect of reducing blood pressure and application of the lactobacillus casei, and belongs to the technical field of functional bacteria. The invention provides lactobacillus casei SLK30, which is preserved in the Guangdong Microbial Culture Collection Center, the address is 59 building, No.100 Courtyard, Xianlie Middle Road, Guangzhou City, the preservation date is March 9, 2022, and the preservation number is GDMCC No: 62280. The Lactobacillus casei SLK30 provided by the invention has ideal ACE inhibition rate and GABA production capability, and is a probiotic strain for lowering blood pressure. Fermentation characteristic research shows that the lactobacillus casei SLK30 has relatively good acid production capacity, acid resistance and bile salt resistance, has a relatively strong inhibition effect on indicator bacteria staphylococcus aureus, escherichia coli and salmonella typhimurium, and can be used as a potential antihypertensive probiotic to be applied to fermented food.

Description

technical field [0001] The invention relates to the technical field of functional bacteria, in particular to a Lactobacillus casei with hypotensive effect and application thereof. Background technique [0002] Hypertension is one of the causes of many cardiovascular diseases. The higher the blood pressure level, the greater the probability of cardiovascular and cerebrovascular diseases. Elevated blood pressure is highly correlated with coronary heart disease, stroke and other diseases. Therefore, effective control of blood pressure is an important intervention to prevent and reduce cardiovascular mortality. Hypertension is a common and frequently-occurring disease that can lead to multiple organ lesions in patients, reduce their quality of life, and seriously threaten or even damage the life safety of patients. main reason. At present, the treatment of hypertension is mainly long-term use of antihypertensive agents. At present, there are many synthetic antihypertensive ag...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23C9/123A23L33/135A61K35/747A61P9/12C12R1/245
CPCA23L33/135A23C9/1234A61K35/747A61P9/12A23V2002/00A23V2400/125A23V2200/326Y02A50/30
Inventor 李玲黄丽杨攀曾庆坤但霞
Owner GUANGXI ZHUANG AUTONOMOUS REGION BUFFALO INST
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