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A kind of bifidobacterium longum and its application

A technology of bifidobacterium longum and bacteria slime, applied in the direction of bifidobacterium longum, application, bacteria, etc., can solve the problem of low survival rate of bifidobacterium longum bacteria powder

Active Publication Date: 2020-10-20
谭瑛
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0018] The second technical problem to be solved by the present invention is the problem that the survival rate of the Bifidobacterium longum bacterial powder obtained by the freeze-dried bacterial powder process in the prior art is low, and then provides a frozen Bifidobacterium longum with a high survival rate of bacterial strains. Preparation method of dry powder

Method used

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  • A kind of bifidobacterium longum and its application
  • A kind of bifidobacterium longum and its application
  • A kind of bifidobacterium longum and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1 Isolation and screening of Bifidobacterium longum

[0045] More than 30 species of bifidobacteria were isolated from the feces of healthy newborn and weaned infants, and in non-allergic children, a bifidobacteria microflora that matched that of adults (or more High more types of bifidobacteria) These bifidobacteria adhere to the mucus in the intestinal tract of children and have a protective effect on the child's body from toxic substances. Therefore, we can increase the content of bifidobacteria in children's food, to Increase children's resistance to disease and allergic reactions to allergic substances.

[0046] The Bifidobacterium longum with the deposit number of CGMCC No. 16126 of the present invention is isolated from the feces of newborns and infants, and is isolated by the following method:

[0047] Put 1 gram of human intestinal feces into a sterilized bottle containing 30 glass balls, add normal saline to make 100-fold dilution, and mix well, and t...

Embodiment 2

[0057] Example 2 Preparation of Bifidobacterium longum lyophilized powder

[0058] The Bifidobacterium longum of the present embodiment is the bacterial strain whose preservation number is CGMCC No. 16126, and it is prepared as Bifidobacterium longum lyophilized powder by the following method:

[0059] (1) Take the Bifidobacterium longum and cultivate it in coagulated milk with an initial pH value of 7.5 at a temperature of 37°C for 36 to 48 hours. % of the inoculum is inoculated on the coagulated cow's milk as a culture medium, and cultivated by shaking flask culture; the coagulated cow's milk can be commercially available coagulated cow's milk, or can be prepared by using cow's milk. For the purpose of the present invention, the medium used is not unique, and those skilled in the art can also replace the coagulated milk with other conventional bifidobacteria medium.

[0060] (2) taking the fermentation broth obtained by fermentation in step (1), placing it in a centrifuge, ...

Embodiment 3

[0062] Example 3 Preparation of Bifidobacterium longum lyophilized powder

[0063] The Bifidobacterium longum of the present embodiment is the bacterial strain whose preservation number is CGMCC No. 16126, and it is prepared as Bifidobacterium longum lyophilized powder by the following method:

[0064] Add a protective agent to the Bifidobacterium longum, mix well, pre-freeze it at a temperature of -30°C for 1 hour, then place it at a temperature of -20°C for sublimation for 10 hours, and then place it at a temperature of -10°C Sublimation for 20 hours, sublimation for 5 hours at a temperature of 30°C, and placing under vacuum for 1 hour to obtain Bifidobacterium longum freeze-dried powder.

[0065] In this embodiment, the protective agent is skimmed milk powder. As an alternative implementation of this embodiment, the protective agent can also be replaced with skim milk powder, maltose, sucrose, soluble starch, mannitol, sodium glutamate, L - one or more of cysteine, gelatin...

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Abstract

The present invention provides a kind of Bifidobacterium longum, which was submitted to the General Microorganism Center (CGMCC) of China Microbiological Culture Collection Management Committee (CGMCC) for preservation on July 17, 2018, and the preservation number is: CGMCC No.16126. The bifidobacterium longum provided by the invention has a strong ability to inhibit pathogenic bacteria and has a high survival rate after being made into freeze-dried powder.

Description

technical field [0001] The invention belongs to the technical field of human microecology, in particular to a Bifidobacterium longum and application thereof. Background technique [0002] Various microorganisms (bacteria) often attach to the human body from different environments, and can settle in a certain location and grow and reproduce offspring. This phenomenon is often called "bacterial colonization". Colonized microorganisms must rely on the continuous supply of nutrients by the human body to grow and reproduce, and then have an impact on the human body. [0003] Generally speaking, the human body has the following immune mechanisms: [0004] a. structural barrier [0005] The "structural barriers" of young children against foreign pathogenic microorganisms include surface barriers and internal barriers. The body surface barrier is composed of the skin covering the body surface and the mucous membrane lining the cavities that communicate with the outside world. It...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/04A23L33/135A23C9/12A61K35/745A61P31/04A61P1/12C12R1/01
CPCA61P1/12A61P31/04A23C9/1203A23L33/135C12N1/04C12N1/20A23V2002/00A61K2035/115C12R2001/01C12N1/205A23V2400/533A23V2200/324A23V2200/3204
Inventor 谭瑛
Owner 谭瑛
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