A strain of lactic acid bacteria capable of absorbing or degrading enterotoxin and its application

A technology of enterotoxin and lactic acid bacteria, applied in the field of lactic acid bacteria, can solve the problems of long effective time, dependence, side effects, etc., achieve good acid and bile salt resistance, and enhance the body's immunity

Active Publication Date: 2017-12-26
山东凤凰生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, detoxification and beauty care products can be divided into four categories according to their characteristics and main functional ingredients: the first category uses rhubarb and other laxatives as the main functional ingredients, which have side effects such as abdominal pain and diarrhea. Long-term use can lead to intestinal dysfunction, such as detoxification and beauty capsule
The second category uses cassia seed, yam and aloe as the main functional ingredients, which have certain side effects. Long-term use of the same medicine will cause dependence, such as Besunyen Changrun Tea
The third category uses prebiotics as the main functional ingredient, does not contain any pharmaceutical ingredients, and uses natural elements extracted from pure grains as the functional ingredient. It has natural laxatives, no abdominal pain, no diarrhea, and no dependence, but it takes a long time to take effect, such as Lijian Sanqing

Method used

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  • A strain of lactic acid bacteria capable of absorbing or degrading enterotoxin and its application
  • A strain of lactic acid bacteria capable of absorbing or degrading enterotoxin and its application
  • A strain of lactic acid bacteria capable of absorbing or degrading enterotoxin and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Screening of lactic acid bacteria with the function of removing enterotoxin

[0026] 1.1 Culture of lactic acid bacteria and preparation of fermentation broth

[0027] Lactic acid bacteria were cultured in MRS liquid medium at 37°C for 24h, centrifuged at 6000r / min at 4°C for 10min, and the supernatant was collected as the fermentation broth.

[0028] 1.2 Determination of tolerance of lactic acid bacteria in different toxins and different concentrations

[0029] Take 2mL of indole, phenol, p-cresol mother liquor and 100mL MRS medium, prepare medium with toxin concentration of 0.1, 0.1, 0.5mg / mL, inoculate lactic acid bacteria 24h fermentation broth (2%) at 12h, 24h, After 32 hours, the living bacteria count was sampled, and the survival rate of lactic acid bacteria was calculated with the culture medium fermentation liquid without toxin as a control. Preparation of mother liquor: phenol (60 degrees temperature from crystal state to liquid), p-cresol, respect...

Embodiment 2

[0032] Embodiment 2 acid resistance test

[0033] Use HCl to adjust the pH value of the normal saline to 1.5, 2.0, 3.0 respectively, sterilize at 115°C for 30 minutes, insert 1% of the inoculum into the bacterial liquid fermented for 24 hours aseptically, and incubate at 37°C for 1h, 2h, After 3 hours, samples were taken to determine the number of viable bacteria, and saline was used as a control. Survival rate (%)=the number of viable bacteria in the bacterial liquid to be tested / the number of viable bacteria in salt water×100.

Embodiment 3

[0034] Embodiment 3 bile salt resistance test

[0035] Bacterial solution was inoculated in the MRS medium with bile salt concentration of 0.1%, 0.2%, 0.3%, (w / v) by 1% (v / v), and the MRS medium without bile salt was used as a control, at 37 After culturing at ℃ for 0h, 2h, 4h, and 6h, samples were taken to count plate colonies, and the survival rate of Lactobacillus was calculated.

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Abstract

The invention discloses a strain of lactic acid bacteria capable of adsorbing or degrading enterotoxin and its application, and its preservation number is CCTCC NO: M2015128. The lactic acid bacteria have the function of absorbing or degrading enterotoxin, and have good acid resistance, bile salt resistance, artificial gastric juice and intestinal juice resistance. It is specifically reflected in: it can absorb or degrade enterotoxin, and has a good ability to remove enterotoxin; it can inhibit the growth of common pathogenic bacteria; it can withstand acid and bile salts and survive in the digestive tract. In specific application, the method is: dissolve the freeze-dried bacteria powder in water at normal temperature and take it orally or directly add it in a certain proportion to make functional food or health care product. The lactobacillus of the present invention is obtained by screening from many lactic acid bacteria, it not only has the ability of high-definition enterotoxin, but also has good performance, and can be used for human health care.

Description

technical field [0001] The invention relates to a lactic acid bacterium with the function of clearing intestinal toxins and its application, which can be applied to functional food and health food, and belongs to the field of biotechnology. Background technique [0002] A toxin is a substance that can interfere with normal physiological activities and disrupt body functions. Pollution from the external environment, unhealthy diet and other factors lead to the production of toxins in the body. Toxins in the human body include free radicals, stool, cholesterol, uric acid, lactic acid, water poisoning and congestion, etc., and endogenous toxins are produced during the metabolic process, such as toxins produced by sugar, fat, and protein metabolic disorders, and toxins produced by the accumulation of stool in the intestine. [0003] The toxins stored in the human body not only disrupt the normal digestion and absorption of the human body, but also circulate to various organs of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23L33/00A61K35/747A61P39/02A61P37/04C12R1/25
Inventor 单宝龙王静任宝涛张颜廷杨婷婷庄金丽
Owner 山东凤凰生物科技股份有限公司
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