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Buffer liquid system and application thereof to extraction of kitasamycin

A buffer system and kitasamycin technology are applied in the preparation of sugar derivatives, sugar derivatives, sugar derivatives, etc., to achieve the effects of wide sources, avoiding phosphorus pollution, and sufficient supply

Active Publication Date: 2010-09-29
浙江普洛生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few studies and reports on kitasarmycin raw materials

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Back Extraction of Kitamycin with Oxalic Acid-Sodium Oxalate Buffer

[0030] Prepare 300ml of sodium hydroxide solution with a concentration of 0.6% (g / ml), adjust the pH to 2.0 with oxalic acid, and prepare an oxalic acid-sodium oxalate buffer solution for later use. The dosage of oxalic acid is 2.2% (g / ml).

[0031] Kitamycin fermentation filtrate 1000ml, potency 10965μ / ml, adjust pH to 9.0 with 30% sodium hydroxide, add 500ml butyl acetate to extract for 10 minutes, use high-speed centrifuge to make it layered, collect butyl acetate phase; Prepare 200ml of oxalic acid-sodium oxalate buffer solution for back extraction, collect the water phase in layers; adjust the pH of the water phase to 4.5 with 10% sodium hydroxide, add 1.0g of activated carbon for decolorization, and filter to obtain the decolorization solution; after the decolorization solution is blown out of the solvent Adjust the pH to 9.0 with 10% sodium hydroxide for crystallization; the crys...

Embodiment 2

[0033] Example 2 Back Extraction of Kitamycin with Acetic Acid-Ammonium Acetate Buffer

[0034] Add 20ml of concentrated ammonia water to 480ml of deionized water to prepare a 4% ammonia solution (ammonia / deionized water), then add 25ml of glacial acetic acid to prepare a buffer solution of acetic acid-ammonium acetate for later use, its pH is 4.2, and the dosage of glacial acetic acid is 5% (g / ml).

[0035]Kitamycin fermentation filtrate 1000ml, potency 10356μ / ml, adjust pH to 9.0 with 30% sodium hydroxide, add 500ml butyl acetate to extract for 10 minutes, use high-speed centrifuge to make it layered, collect butyl acetate phase; Prepare 300ml of acetic acid-ammonium acetate buffer solution for back extraction, and collect the water phase in layers; adjust the pH of the water phase to 4.5 with 10% sodium hydroxide, add 1.0g of activated carbon for decolorization, and filter to obtain the decolorization solution; after the decolorization solution is blown out of the solvent...

Embodiment 3

[0036] Example 3 Back Extraction of Kitamycin with Low Concentration Oxalic Acid-Sodium Oxalate Buffer

[0037] Prepare 500ml of 0.2% (g / ml) sodium hydroxide solution, adjust the pH to 1.5 with oxalic acid, and make oxalic acid-sodium oxalate buffer solution for later use, and the dosage of oxalic acid is 0.55% (g / ml).

[0038] Kitamycin fermentation filtrate 1000ml, potency 10356μ / ml, adjust pH to 9.0 with 30% sodium hydroxide, add 500ml butyl acetate to extract for 10 minutes, use high-speed centrifuge to make it layered, collect butyl acetate phase; Prepare 500ml of oxalic acid-sodium oxalate buffer solution for back extraction, collect the water phase in layers; adjust the pH of the water phase to 4.5 with 10% sodium hydroxide, add 1.0g of activated carbon for decolorization, and filter to obtain the decolorization solution; after the decolorization solution is blown out of the solvent Adjust the pH to 9.0 with 10% sodium hydroxide for crystallization; the crystals were...

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PUM

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Abstract

The invention belongs to the field of antibiotic medicaments, and provides a new buffer liquid system for extraction of kitasamycin. The buffer liquid system is solution mainly containing unitary or polybasic carboxylic acid having less than 10 carbon atoms and soluble salt thereof, wherein pH ranges from 1.5 to 4.2. The buffer liquid system can effectively solve the problem that a large amount of phosphorus element is brought into production to cause over high phosphorus content in waste water because phosphoric acid buffer liquid is generally used for back extraction of the kitasamycin in aconventional process. The system greatly increases the problem of pressure on environment-friendly treatment and can effectively prevent the phosphorus element from being brought into the production to relieve the pressure on environment-friendly dephosphorization.

Description

technical field [0001] The invention belongs to the field of antibiotic drugs, in particular to a method for extracting kitasamycin. Background technique [0002] Kitamycin, also known as leucomycin, is a sixteen-membered macrolide antibiotic, including components A1, A3, A4, A5, A6, A7, A8 and A9. At present, the method commonly used in the extraction of kitasamycin is: filter the kitasamycin fermentation liquid after pretreatment to obtain the kitasamycin filtrate; then add a hydrophobic organic solvent (such as ethyl acetate, butyl acetate, etc.) to the kitasamycin filtrate ), extracting kitasamycin into the organic solvent phase; then back-extracting the organic solvent phase with phosphate buffer to obtain kitasamycin phosphate buffer; adjusting the pH of the solution to obtain kitasarmycin crystals, and drying to obtain the finished product. [0003] The phosphorus content in the production wastewater treated by the above process is as high as 500-800mg / L, but the pho...

Claims

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Application Information

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IPC IPC(8): C07H17/08C07H1/06
Inventor 许永兴
Owner 浙江普洛生物科技有限公司
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