Buffer liquid system and application thereof to extraction of kitasamycin
A buffer system and kitasamycin technology are applied in the preparation of sugar derivatives, sugar derivatives, sugar derivatives, etc., to achieve the effects of wide sources, avoiding phosphorus pollution, and sufficient supply
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Embodiment 1
[0029] Example 1 Back Extraction of Kitamycin with Oxalic Acid-Sodium Oxalate Buffer
[0030] Prepare 300ml of sodium hydroxide solution with a concentration of 0.6% (g / ml), adjust the pH to 2.0 with oxalic acid, and prepare an oxalic acid-sodium oxalate buffer solution for later use. The dosage of oxalic acid is 2.2% (g / ml).
[0031] Kitamycin fermentation filtrate 1000ml, potency 10965μ / ml, adjust pH to 9.0 with 30% sodium hydroxide, add 500ml butyl acetate to extract for 10 minutes, use high-speed centrifuge to make it layered, collect butyl acetate phase; Prepare 200ml of oxalic acid-sodium oxalate buffer solution for back extraction, collect the water phase in layers; adjust the pH of the water phase to 4.5 with 10% sodium hydroxide, add 1.0g of activated carbon for decolorization, and filter to obtain the decolorization solution; after the decolorization solution is blown out of the solvent Adjust the pH to 9.0 with 10% sodium hydroxide for crystallization; the crys...
Embodiment 2
[0033] Example 2 Back Extraction of Kitamycin with Acetic Acid-Ammonium Acetate Buffer
[0034] Add 20ml of concentrated ammonia water to 480ml of deionized water to prepare a 4% ammonia solution (ammonia / deionized water), then add 25ml of glacial acetic acid to prepare a buffer solution of acetic acid-ammonium acetate for later use, its pH is 4.2, and the dosage of glacial acetic acid is 5% (g / ml).
[0035]Kitamycin fermentation filtrate 1000ml, potency 10356μ / ml, adjust pH to 9.0 with 30% sodium hydroxide, add 500ml butyl acetate to extract for 10 minutes, use high-speed centrifuge to make it layered, collect butyl acetate phase; Prepare 300ml of acetic acid-ammonium acetate buffer solution for back extraction, and collect the water phase in layers; adjust the pH of the water phase to 4.5 with 10% sodium hydroxide, add 1.0g of activated carbon for decolorization, and filter to obtain the decolorization solution; after the decolorization solution is blown out of the solvent...
Embodiment 3
[0036] Example 3 Back Extraction of Kitamycin with Low Concentration Oxalic Acid-Sodium Oxalate Buffer
[0037] Prepare 500ml of 0.2% (g / ml) sodium hydroxide solution, adjust the pH to 1.5 with oxalic acid, and make oxalic acid-sodium oxalate buffer solution for later use, and the dosage of oxalic acid is 0.55% (g / ml).
[0038] Kitamycin fermentation filtrate 1000ml, potency 10356μ / ml, adjust pH to 9.0 with 30% sodium hydroxide, add 500ml butyl acetate to extract for 10 minutes, use high-speed centrifuge to make it layered, collect butyl acetate phase; Prepare 500ml of oxalic acid-sodium oxalate buffer solution for back extraction, collect the water phase in layers; adjust the pH of the water phase to 4.5 with 10% sodium hydroxide, add 1.0g of activated carbon for decolorization, and filter to obtain the decolorization solution; after the decolorization solution is blown out of the solvent Adjust the pH to 9.0 with 10% sodium hydroxide for crystallization; the crystals were...
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