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Ultraviolet spectrophotometric detection method for kitasamycin content

A guitarmycin and spectrophotometric technology, applied in the measurement of color/spectral characteristics, etc., can solve the problems of urgent residue detection, hidden dangers to human health, etc., and achieve the effect of saving detection time, high accuracy and improving production efficiency.

Inactive Publication Date: 2017-04-26
JIANGSU WISE SCI & TECH DEV
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Problems solved by technology

However, its residues in animal-derived foods have brought great hidden dangers to human health.
Therefore, residue detection is urgent

Method used

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  • Ultraviolet spectrophotometric detection method for kitasamycin content
  • Ultraviolet spectrophotometric detection method for kitasamycin content

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Embodiment Construction

[0016] The present invention will be described in further detail below in combination with specific embodiments.

[0017] Kitamycin produced by Anhui Renhe Pharmaceutical Co., Ltd. was selected as a standard sample for analysis. The ultraviolet spectrophotometer used was Shanghai Tianmei UV2300 II spectrophotometer, and the detection wavelength was 215 nm. The detection process is as follows:

[0018] 1. Standard solution preparation:

[0019] Preparation of 20mg / L solution: Precisely measure 0.5g of kitasarmycin into a 250m1 volumetric flask, add water to dissolve, and bring the volume up to the mark, take 5ml into a 500ml volumetric flask, add water to make up the volume to the mark, and you get it.

[0020] Preparation of 40mg / L solution: Precisely measure 0.1 g of kitasamycin into a 250m1 volumetric flask, add water to dissolve, and bring the volume up to the mark, take 10ml into a 100m1 volumetric flask, add water to make up the volume to the mark, and you get it.

[00...

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Abstract

Belonging to the technical field of kitasamycin content detection, the invention discloses an ultraviolet spectrophotometric detection method for kitasamycin content. The method includes preparation of a standard solution, determination of a regression equation, sample detection and other processes. The method has short detection time, the detection process can be restored, and the detection process only uses water as the solvent, therefore the method has the characteristics of green and environmental protection, safety and no toxicity.

Description

technical field [0001] The invention relates to the technical field of kitasamycin content detection. Background technique [0002] Kitasamycin, also known as leucomycin, is a multi-component macrolide antibiotic produced by Streptomyces kit asatoensis, containing A1, A3, A4, A5, etc. 9 Group A and 4 Group B components. It has strong antibacterial activity against most Gram-positive bacteria and some Gram-negative bacteria and mycoplasma[3], and is used to prevent bacterial infections and mycoplasma diseases in livestock and poultry, as well as as a growth-promoting agent. Kitamycin has a strong bitter taste, which can affect the palatability of animals and stimulate the stomach. Its alkaline characteristics make it degrade in the acidic environment of the stomach (p H ≤ 5.5) and reduce its activity. In order to avoid the above shortcomings, it has been successfully developed Kitamycin microcapsule preparation (coated with enteric material). However, its residues in anima...

Claims

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Application Information

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IPC IPC(8): G01N21/33
Inventor 洪霞刘静立雯馨
Owner JIANGSU WISE SCI & TECH DEV
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