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Kitasamycin fermenting culture medium

A fermentation medium and kitasamycin technology, applied in fermentation and other directions, can solve problems such as high residual oil, slow titer growth, and decreased vitality, and achieve the effects of increased extraction yield, improved product quality, and stable production

Inactive Publication Date: 2017-05-03
SHANDONG DONGYAO PHARMACEUTICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the oil in the culture medium is not well utilized, a large number of accumulated oil particles will adhere to the mycelia, affecting the uptake of oxygen and nutrients by the bacteria, and the aging speed of the mycelia will be fast, and some mycelia will autolyze and release ammonia nitrogen. When the pH rises, the precursor substances required for product synthesis cannot be formed smoothly, and the continuous rise of pH and the aging of the bacteria also affect the decline in the activities of various enzymes required for biosynthesis, and the biosynthesis of products is inhibited, showing slow growth in potency , it will even drop in the later stage, and normal fermentation production cannot be carried out
The oil in the existing fermentation medium formula basically directly floats on the surface of the fermentation liquid, and it is only mixed with other substances by stirring, which is not conducive to the utilization of bacteria, and it is easy to cause abnormal batches of fermentation, which eventually leads to low titer of the fermentation liquid , the amount of residual oil is high, and the amount of emulsification increases during the extraction process, which affects the extraction yield and product quality

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Slant and seed culture: Infectious slant was generated with kitasamycin. Slant medium: starch 1.0-2.0%, magnesium sulfate 0.04-0.06%, potassium dihydrogen phosphate 0.04-0.06%, yeast extract 0.3-0.5%, agar 2.0-2.2%; pH7.0-7.5 before elimination. Cultivate at a constant temperature of 29±0.5°C for 8-10 days, use a mature slant to access the seed tank for cultivation, and the inoculation amount is 1 / 6-1 / 5 slant / 20L seed medium. Seed medium: starch 1.5-2.5%, bean cake powder 1.8-2.3%, glucose 0.8-1.2%, sodium chloride 0.3-0.5%, calcium carbonate 0.2-0.4%, pH7.0-7.5 before digestion. Cultivate for 30-35 hours at 0.5°C, rotation speed 150rpm, and air volume 1:1. Fermentation culture: transfer the mature seed liquid into a fermenter for cultivation, and the inoculation amount is 10-15%. Under the conditions of temperature 29-31°C, rotation speed 200-260rpm, air volume 1:0.7-0.9, ferment and cultivate for 65-75h. The fermentation medium used is: starch 1.5-4.5%, glucose 0.1...

Embodiment 2

[0020] The emulsifier Tween 80 was added to the soybean oil according to the proportion of 0.3% of the total amount of the fermented medium, and then added to the medium after stirring evenly, and the rest were the same as in Example 1.

[0021] After the fermentation, it was measured that the potency of kitasamycin was 13500u / ml, the potency of the finished product was 1670ug / mg, and the yield was 82%, which was 1.22 times higher than that of the control without emulsifier, and the potency of the finished product was also improved.

Embodiment 3

[0023] The emulsifier Tween 80 was added to the soybean oil according to the proportion of 0.5% of the total amount of the fermented medium, and then added to the medium after stirring evenly, and the rest were the same as in Example 1.

[0024] After the fermentation, the potency of kitasamycin was measured to be 13360u / ml, the potency of the finished product was 1656ug / mg, and the yield was 81.6%, which was 1.21 times higher than that of the control without emulsifier, and the potency of the finished product was also improved.

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PUM

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Abstract

The invention relates to a Kitasamycin fermenting culture medium and belongs to the field of production of kitasamycin. The fermenting culture medium comprises the components in percentages by weight: 1.5-4.5% of starch, 0.1-0.3% of glucose, 2.0-4.0% of soybean cake powder, 0.5-1.5% of fibroin powder, 0.2-0.5% of calcium carbonate, 0.006-0.015% of amylase, 2.0-3.5% of soya-bean oil, 0.01-0.06% of GPE, 0.05-0.1% of potassium dihydrogen phosphate, 0.15-0.55% of ammonium sulfate, 0.04-0.06% of manganese chloride, 0.005-0.007% of zinc sulfate as well as an emulsifier which is tween 80. The tween 80 is uniformly mixed with the soy-bean oil and is then mixed with other comments; the pH of the fermenting culture medium is 6.3-7.5. By using the culture medium, the fermenting level of kitasamycin can be improved. Moreover, after fermentation of the culture medium, oil in fermenting liquor is fully used, so that the use level of the soy-bean oil is reduced, and the production cost is lowered; generation of emulsification in the extraction process is further reduced to facilitate extraction of a product in a purifying section, so that the extraction yield is improved, and the product quality is improved to a great extent.

Description

technical field [0001] The invention relates to the field of kitasamycin production, in particular to a kitasarmycin fermentation medium. Background technique [0002] Kitamycin is a multicomponent macrolide antibiotic produced by Streptomyces. Its free base is for oral use; the tartrate salt is for injection. Its salt is white or light yellow crystalline powder, odorless, bitter, slightly hygroscopic; easily soluble in water, methanol or ethanol; almost insoluble in ether and chloroform. [0003] Kitamycin has similar antibacterial properties to erythromycin, and has a strong inhibitory effect on Gram-positive bacteria, such as Staphylococcus, Streptococcus pyogenes, Streptococcus viridans, Streptococcus pneumoniae, Bacillus tetanus, and Bacillus diphtheria. It also has considerable inhibitory effect on Gram-negative bacteria, such as Neisseria gonorrhoeae and Bacillus pertussis. In addition, it has inhibitory effect on mycoplasma, leptospira and rickettsia. Effective a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/62
Inventor 张丽丽于新令
Owner SHANDONG DONGYAO PHARMACEUTICAL CO LTD
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