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Brassica campestris ssp. Chinensis cytoplasmic male sterile gene molecular marking method

A technology for cytoplasmic male sterility and head cabbage, which is applied in the directions of biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc. Convenient, cost-effective effect

Active Publication Date: 2007-03-28
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

But so far there is no report on cytoplasmic male sterility gene marker screening and marker-assisted selection of non-heading Chinese cabbage. Molecular marker-assisted selection is not limited by environmental conditions, and can achieve seedling selection, reduce workload, and improve selection efficiency, thereby speeding up the process of selection. Breeding process

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  • Brassica campestris ssp. Chinensis cytoplasmic male sterile gene molecular marking method

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Embodiment Construction

[0040] Using molecular marker technology such as RAPD to search for markers linked to sterility genes provides an extremely useful tool for locating and cloning these genes, and lays a good foundation for molecular marker-assisted breeding. Molecular marker-assisted selection is not restricted by environmental conditions, and can realize seedling selection, reduce workload, and speed up the breeding process. At present, the study on molecular markers of CMS gene in non-heading Chinese cabbage is still blank.

[0041] Implementation procedure of the present invention is:

[0042] (1) Materials and methods Non-heading Chinese cabbage cytoplasmic male sterile line 98HA and its maintainer line 98HB, a near-isogenic line, were obtained from the Vegetable Research Institute of Nanjing Agricultural University (Hou X.L.Cao S.C and He Y.K.Creation of a new germplasm of CMS non -heading Chinese cabbage. Acta Horticulturae, 2004, 1: 75-81, the Vegetable Research Institute of Nanjing Agr...

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Abstract

The invention relates to the molecule marking method for non-heading cabbage male sterile gene that takes selecting of PAPD and SCAR molecule mark for non-heading cabbage male sterile series 98HA and maintainer line 98HB to gain two marks of non-heading cabbage male sterile genes--NAU / RAPD / CMS1800 and NAU / RAPD / CMS2600 and transforming it to two SCAR marks NAU / SCAR / CMS3800 and NAU / SCAR / CMS4600. The invention could improve selecting efficiency and speed up breeding process.

Description

1. Technical field [0001] The molecular marking method of cytoplasmic male sterile gene of non-heading Chinese cabbage of the present invention belongs to the field of biotechnology, relates to the molecular breeding of cytoplasmic male sterile line of non-heading Chinese cabbage, and is specially used for the cytoplasmic male sterile gene of non-heading Chinese cabbage filter. 2. Technical background [0002] Non-heading cabbage (Brassica campestris ssp.chinensis Makino), native to China, is the most popular vegetable widely grown in southern my country, and plays a pivotal role in the annual supply of vegetables (Hou Xilin, 2003). Non-heading Chinese cabbage is a typical cross-pollination crop with obvious heterosis, and the use of male sterile lines is an ideal material for hybrid production. [0003] Cytoplasmic male sterility is a type of male sterility inherited maternally. It plays an important role in the utilization of plant heterosis, and its in-depth study has gr...

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11C12P19/34
Inventor 侯喜林史公军
Owner NANJING AGRICULTURAL UNIVERSITY
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