Active near infrared fluorophore as well as preparation method and application thereof

A fluorophore, near-infrared technology, applied in chemical instruments and methods, luminescent materials, pharmaceutical formulations, etc., can solve problems such as no near-infrared fluorescent probe reports, and achieve suitable fluorescence wavelength, stable labeling, absorption coefficient and The effect of high quantum yield
CN104673273AInactive Publication Date: 2015-06-03FUDAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
FUDAN UNIV
Publication Date
2015-06-03
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention belongs to the field of molecular imaging reagents ad relates to an active near infrared fluorophore for rapidly marking bioactive molecules. A general formula of the active near infrared fluorophore is IRP-B-NHS, wherein IRP is an anthocyanin near infrared fluorophore; B is an aromatic group introduced to the secondary position of the anthocyanin fluorophore by virtue of a carbon-carbon bond; NHS is N-hydroxysuccinimide eater. A preparation method of the active near infrared fluorophore comprises the following steps: with the anthocyanin fluorophore as a parent, introducing benzene carboxylic acid into the fluorophore by virtue of the carbon-carbon bond through Suzuki-Miyaura reaction, modifying phenyl carboxylic acid to produce the N-hydroxysuccinimide eater, reacting with primary amine in a biomolecule under the physiological condition, and marking the biomolecule with the near infrared fluorophore to realize noninvasive tracing. The active near infrared fluorophore is capable of rapidly, safely, effectively and stably marking the bioactive molecules including polypeptide, proteins, antibodies or polymer molecules and has important significance of noninvasively monitoring and quantifying distribution of target active molecules in vivo.
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Description

technical field

[0001] The invention belongs to the field of molecular imaging reagents, relates to a class of active near-infrared fluorescent groups that can rapidly label bioactive molecules, and specifically relates to the synthesis, characterization, optical properties and application of such active near-infrared fluorescent groups in biomolecular labeling Applications. This type of fluorophore has the characteristics of high labeling efficiency, mild labeling conditions, high fluorophore absorption coefficient and quantum yield, and stable photochemical properties. The non-invasive monitoring and quantification of the distribution of target bioactive molecules in vivo can be realized through the labeling of such fluorescent groups. Background technique

[0002] The optical microscope in the sixteenth century changed people's understanding of biology and promoted the rapid development of biomedicine. It is known in the art that the size and shape of cells, organelles,...

Claims

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