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Biogenic insecticide mixture controlling pests, preparation method and application

A technology for preventing and controlling pests and biological sources. It is applied in the fields of botanical equipment and methods, pesticides, and applications. It can solve the problems of insufficient insecticidal control effect and low virulence, and overcome the long residual time and high virulence. , the effect of reducing the use of

Inactive Publication Date: 2017-08-25
杨飞
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the above-mentioned patent directly adopts a fermented liquid of Bacillus thuringiensis, the toxicity of which is 12530U / mg, which only reaches the level of the national pesticide standard.

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0033] A biological insecticidal compound for preventing and controlling pests. The preparation raw materials include the following components by weight: 50 parts of Bacillus thuringiensis fermentation broth, 15 parts of lactic acid bacteria fermentation broth, 15 parts of fresh three-part three leaves, 15 parts of fresh camphor leaves , Tween 20 0.05 parts. The Bacillus thuringiensis is Bacillus thuringiensis 8010, and the lactic acid bacterium is Tetragenococcus halophilus, which is specifically prepared according to the following steps:

[0034] Step 1. Preparation of Bacillus thuringiensis fermentation broth

[0035] Step 1.1, activate the Bacillus thuringiensis strain, and culture it with beef extract peptone liquid medium in a shake flask to obtain a Bacillus thuringiensis seed liquid; the shake flask culture conditions are temperature 30°C, rotation speed 220r / min, and culture time 12h;

[0036] Step 1.2, soak the soybeans in water for 5 hours, drain the water, then steam the...

Embodiment 2

[0047] A biological insecticidal compound for preventing and controlling pests. The preparation raw materials include the following components by weight: 40 parts of Bacillus thuringiensis fermentation broth, 10 parts of lactic acid bacteria fermentation broth, 20 parts of fresh three-part three leaves, and 20 parts of fresh camphor leaves , Tween 20 0.07 parts. The Bacillus thuringiensis is Bacillus thuringiensis 8010, and the lactic acid bacteria are Bifidobacterium, which are specifically prepared according to the following steps:

[0048] Step 1. Preparation of Bacillus thuringiensis fermentation broth

[0049] Step 1.1, activate the Bacillus thuringiensis strains, and use beef extract peptone liquid medium to shake flask culture to obtain Bacillus thuringiensis seed liquid;

[0050] Step 1.2, soak the soybeans in water for 6 hours, drain the water, then steam them, and cool to 30-40°C to obtain cooked soybeans; grind wheat stalks into powder and pass through a 60-mesh sieve to ...

Embodiment 3

[0062] A biological insecticidal compound for preventing and controlling pests. The preparation raw materials include the following components by weight: 60 parts of Bacillus thuringiensis fermentation broth, 20 parts of lactic acid bacteria fermentation broth, 10 parts of fresh three-part three leaves, and 10 parts of fresh camphor leaves , Tween 20 0.1 parts. The Bacillus thuringiensis is Bacillus thuringiensis 8010, and the lactic acid bacteria are Tetragenococcus halophilus (Tetragenococcus halophilus). The preparation method is the same as that of Example 1, except that the formula is different. Dilute the compound solution 2000 times before use, spray 50kg of the diluted solution per mu of cauliflower field, and no aphids, diamondback moths and other diseases and insect pests were seen within two months of spraying.

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PUM

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Abstract

The invention discloses a biogenic insecticide mixture controlling pests. The biogenic insecticide mixture is prepared from, by weight, 40-60 parts of a bacillus thuringiensis fermentation solution, 10-20 parts of a lactic acid bacteria fermented solution, 10-20 parts of fresh anisodus acutangulus leaves, 10-20 parts of fresh leaves of camphor trees and 0.05-0.1 part of Tween 20. According to the biogenic insecticide mixture, the selectivity of selected bacillus thuringiensis is high, damage to people and livestock does not exist, the biogenic insecticide mixture belongs to biological insecticide sources very high in safety, lactic acid bacteria belong to probiotics, the lactic acid bacteria are used for preparing an insecticide for the first time, the toxicity titer is high, time needed for disinsection is short, a preservative does not need to be additionally added, chemical substances are reduced, and the problem that residual time of chemical pesticide is long is solved. In addition, the invention discloses a preparation method and application of the biogenic insecticide mixture.

Description

Technical field [0001] The invention belongs to the technical field of insecticides, and specifically relates to a biological insecticidal compound for preventing and curing insects, a preparation method and an application. Background technique [0002] Vegetables are one of the indispensable foods in people’s lives. They contain nutrients such as vitamins and minerals needed by the human body, which are closely related to people’s lives. Aphids and whiteflies are the most common pests and diseases that endanger the growth of vegetables. In severe cases, it will affect the growth of vegetables, resulting in a large reduction in vegetable production. [0003] Most of the existing pesticides used to control aphids and whiteflies are Actai, Kuaishaling, Sweeping Cleaner, and Aphid Cleaner. However, these pesticides are all chemical pesticides, and chemical pesticides are not easily degraded and easily cause pesticides in vegetables. The residual time is long and there is a risk of ha...

Claims

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Application Information

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IPC IPC(8): A01N65/38A01N65/24A01N63/02A01P7/04
CPCA01N63/10A01N65/00A01N65/24A01N65/38Y02A50/30
Inventor 杨飞李继光
Owner 杨飞
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