Thuringiensis bacillus strain for killing beet noctuids and its preparing process

A technology of Bacillus chrysogenum and beet armyworm, applied in botany equipment and methods, bacteria, biocides, etc., can solve problems such as loss, single species, and small control effect, and achieve significant innovation and industrial value.

Inactive Publication Date: 2004-03-10
WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

However, BT insecticides are mainly used to control sensitive Lepidoptera pests, and the variety is single, which seriously affects the effective use of BT insecticides.
[0003] In recent years, the beet armyworm (Spodoptera exigua) is the main pest that harms vegetables, cotton, wheat and other crops, causing huge losses to my country's agricultural production every year, and the resistance of this pest to chemical insecticides is increasing day by day. The control effect of chemical pesticides is also very little, and the commercialized BT insecticides are basically ineffective against it

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0021] 1. Bacteria isolation and culture

[0022] A little soil sample was put into a test tube containing 4.5ml of nutrient culture medium, and incubated at 78°C in a water bath for 15 minutes and shaken for 48 hours. Dilute the nutrient medium to 10 -7 -10 -8 times, spread on the separation medium, incubate incubator at 30°C for 3 days, and select single colonies producing parasporal crystals by microscopic examination.

[0023] Nutrient medium: peptone 1% beef extract 0.3% NaCl 0.5% PH7.5. mass percentage.

[0024] Separation medium: peptone 1% beef extract 0.3% NaCl 0.5% agar 2% PH7.5. mass percentage.

[0025] 2. Strain shake flask fermentation

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Abstract

A Bacillus thuringiensis WY-190 (CCTCC No.M202005) for killing beet noctuids is prepared through loading soil sample in test tube containing nutritive liquid, vibration culture, diltuing, coating on discrete culture medium, inversion culture, purifying monoclonly, inoculating to fermenting culture medium, shake-flask fermentation until parasporal crystal is dropped, mixing with artificial feed, and biological test with target insect. Its advantage is high insecticiding toxin.

Description

technical field [0001] The invention relates to a Bacillus thuringiensis strain with high efficiency for killing Spodoptera exigua. Bacillus thuringiensis WY-190 strain has extremely high toxicity to beet armyworm larvae. It also relates to the preparation method of the strain. Background technique [0002] Bacillus thuringiensis (BT for short) has been researched and developed in my country for more than 40 years. Practice has proved that it has a good control effect on more than 50 common crop pests, and plays an important role in the control of diseases and insect pests. played an important role. However, BT pesticides are mainly used to control sensitive Lepidoptera pests, and the species is single, which seriously affects the effective utilization of BT pesticides. [0003] In recent years, the beet armyworm (Spodoptera exigua) is the main pest that harms vegetables, cotton, wheat and other crops, causing huge losses to my country's agricult...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/00C12N1/20
Inventor 阎建平牛桂兰郑大胜袁志明罗绍彬
Owner WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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