Thuringiensis bacillus strain for killing beet noctuids and its preparing process
A technology of Bacillus chrysogenum and beet armyworm, applied in botany equipment and methods, bacteria, biocides, etc., can solve problems such as loss, single species, and small control effect, and achieve significant innovation and industrial value.
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[0021] 1. Strain isolation and cultivation
[0022] Put a little soil sample into a test tube containing 4.5ml of nutrient solution, and culture it with shaking for 48 hours at 78°C in a water bath for 15 minutes. Dilute the culture medium to 10 -7 -10 -8 times, spread it on the separation medium, culture it upside down in a 30°C incubator for 3 days, and select a single colony producing paraspora crystals by microscopic examination.
[0023] Nutrient medium: 1% peptone, 0.3% beef extract, 0.5% NaCl, PH7.5.
[0024] Separation medium: 1% peptone, 0.3% beef extract, 0.5% NaCl, 2% agar, PH7.5.
[0025] 2. Strain shake flask fermentation
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