Thuringiensis bacillus strain for killing beet noctuids and its preparing process
A technology of Bacillus chrysogenum and beet armyworm, applied in botany equipment and methods, bacteria, biocides, etc., can solve problems such as loss, single species, and small control effect, and achieve significant innovation and industrial value.
Inactive Publication Date: 2002-09-11
WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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Problems solved by technology
However, BT insecticides are mainly used to control sensitive Lepidoptera pests, and the variety is single, which seriously affects the effective use of BT insecticides.
[0003] In recent years, the beet armyworm (Spodoptera exigua) is the main pest that harms vegetables, cotton, wheat and other crops, causing huge losses to my country's agricultural production every year, and the resistance of this pest to chemical insecticides is increasing day by day. The control effect of chemical pesticides is also very little, and the commercialized BT insecticides are basically ineffective against it
Method used
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Experimental program
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Embodiment Construction
[0021] 1. Strain isolation and cultivation
[0022] Put a little soil sample into a test tube containing 4.5ml of nutrient solution, and culture it with shaking for 48 hours at 78°C in a water bath for 15 minutes. Dilute the culture medium to 10 -7 -10 -8 times, spread it on the separation medium, culture it upside down in a 30°C incubator for 3 days, and select a single colony producing paraspora crystals by microscopic examination.
[0023] Nutrient medium: 1% peptone, 0.3% beef extract, 0.5% NaCl, PH7.5.
[0024] Separation medium: 1% peptone, 0.3% beef extract, 0.5% NaCl, 2% agar, PH7.5.
[0025] 2. Strain shake flask fermentation
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A Bacillus thuringiensis WY-190 (CCTCC No.M202005) for killing beet noctuids is prepared through loading soil sample in test tube containing nutritive liquid, vibration culture, diltuing, coating on discrete culture medium, inversion culture, purifying monoclonly, inoculating to fermenting culture medium, shake-flask fermentation until parasporal crystal is dropped, mixing with artificial feed, and biological test with target insect. Its advantage is high insecticiding toxin.
Description
technical field [0001] The invention relates to a bacillus thuringiensis strain with high efficiency for killing beet armyworm. Bacillus thuringiensis WY-190 strain has a very high poisonous effect on beet armyworm larvae. It also relates to the preparation method of the bacterial strain. Background technique [0002] Bacillus thuringiensis (abbreviated as BT) insecticide has been researched and developed in my country for more than 40 years. Practice has proved that it has a good control effect on more than 50 kinds of recurrent pests of crops. It plays an important role in the control of diseases and insect pests. played an important role. However, BT insecticides are mainly used to control sensitive Lepidoptera pests, and the variety is single, which seriously affects the effective use of BT insecticides. [0003] In recent years, the beet armyworm (Spodoptera exigua) is the main pest that harms vegetables, cotton, wheat and other crops, causing ...
Claims
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Inventor 阎建平牛桂兰郑大胜袁志明罗绍彬
Owner WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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