Strain for improving intestinal tracts of aquatic animals and application thereof

A technology of aquaculture and bacterial strains, applied in the field of microorganisms, can solve the problem of single function of probiotics, achieve the effect of reducing bait coefficient, improving survival rate, and good production and application prospects

Inactive Publication Date: 2015-05-06
THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the reported probiotics used in aquaculture have relatively single functions. Screening and isolating probiotics with multiple functions can create huge economic benefits, which has important research significance.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0023] 2. Preparation of probiotic preparations:

[0024] Using strain FA08 to prepare a probiotic preparation capable of improving the intestines of aquaculture animals, the operation steps are as follows:

[0025] (1) Strain culture: Take strain FA08 and inoculate it into LB medium, and activate and cultivate it at 28-40℃, 150-200rpm for 5-10h; after activation, the seed solution is obtained, and then inoculated at 1-10% Inoculate the seed liquid into LB medium, and incubate at 28-40°C and 150-200rpm for 12-24h;

[0026] (2) Strain concentration: Centrifuge the bacterial liquid obtained in step (1) at 8000-10000 rpm for 5-10 minutes, discard the supernatant to obtain a wet bacterial liquid;

[0027] (3) Strain fixation: add diatomaceous earth or medical stone powder or zeolite powder 50g / L-2000g / L with a fineness of 120-250 mesh to the obtained wet bacteria liquid, then dry at 30-40℃, and finally The finished product of the probiotic preparation is obtained by sub-packaging and pac...

Embodiment 1

[0031] Strain culture: Take strain FA08 and inoculate it into LB medium, and activate and cultivate it at 30℃ and 150rpm for 6h; after activation, the seed solution is obtained, and then the seed solution is inoculated into LB medium according to 1% inoculum, and Cultivate for 20 hours at 30°C and 150 rpm to obtain bacterial solution for later use;

[0032] Strain concentration: Centrifuge the obtained bacterial liquid at 10000rpm for 6min, discard the supernatant to obtain a wet bacterial liquid;

[0033] Strain fixation: adding 200 g / L of diatomaceous earth with a crushing fineness of 120-250 mesh to the obtained wet bacteria liquid, then drying at 30° C., and finally packaging and packaging to obtain the finished product of the probiotic preparation.

Embodiment 2

[0035] Strain culture: Take the strain FA08 and inoculate it into LB medium, and activate and cultivate it at 28℃ and 200rpm for 10h; after activation, the seed solution is obtained, and then the seed solution is inoculated into LB medium according to 10% inoculum, and Cultivate for 24 hours at 40°C and 200 rpm to obtain bacterial solution for later use;

[0036] Strain concentration: Centrifuge the obtained bacterial liquid at 9000 rpm for 5 min, discard the supernatant to obtain a wet bacterial liquid;

[0037] Strain fixation: adding 1000 g / L of maifan stone powder with a crushing fineness of 120-250 mesh to the obtained wet bacteria solution, then drying at 40°C, and finally packaging and packaging to obtain the finished product of the probiotic preparation.

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Abstract

The invention discloses a strain for improving the intestinal tracts of aquatic animals. The strain is separated and selected from a substrate sludge sample from a culture pond of Ningde city in Fujian province, and the strain is Bacillus licheniformis FA08 which is collected in the China General Microbiological Culture Collection Center (CGMCC) with the collection number of CGMCC No: 9544 on August 25, 2014. In addition, the invention further discloses a method for preparing a probiotic preparation for improving the intestinal tracts of aquatic animals from the strain. The prepared microbial preparation has a good effect of inhibiting aquatic pathogenic bacteria such as vibrio alginolyticus and aeromonas hydrophila, can produce a variety of active enzymes, can reduce the feed coefficient of the aquatic animals and increase the survival rate of the aquatic animals and has good production and application prospect.

Description

Technical field [0001] The invention belongs to the technical field of microorganisms, and specifically relates to a strain for improving the intestinal tract of aquaculture animals and its application. Background technique [0002] With the rapid development of my country's aquaculture industry, the huge economic benefits brought by high-density aquaculture have made it the main aquaculture mode for most economic fish. However, poor management of high-density aquaculture mode can easily lead to many problems such as frequent breeding diseases and serious deterioration of aquaculture water bodies. For this reason, farmers have turned their attention to the probiotics that have emerged in recent years while increasing the breeding density. Put probiotics to control the number of pathogenic bacteria in the water body and purify the water quality, thereby reducing the occurrence of breeding diseases. Application of Bacillus in aquaculture Since Kozasa of Japan first applied Bacillu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K1/16C12R1/10
CPCC12N1/205C12R2001/10
Inventor 徐长安唐旭刘源森林凌黄仕新方卫东
Owner THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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