Mixed fermentation method of bacillus and bacterial enzymes for efficiently degrading soybean meal antigenic protein

Abstract

The invention discloses a mixed fermentation method of bacillus and bacterial enzymes for efficiently degrading soybean meal antigenic protein. A group of bacillus bacteria that can efficiently degrade soybean meal antigenic protein, including two bacillus strains belonging to Bacillus subtilis subsp.subtilis, are preserved in the General Microorganism Center of China Committee for the Collection of Microbial Cultures, and the preservation numbers are CGMCC NO: 12823, CGMCC NO: 12824. The method of bacteria and enzyme mixed fermentation to degrade the anti-nutritional factors of feed is to make corn, soybean meal and bran into compound feed, add Bacillus fermented seed liquid at 3-5% of the total mass, and add non-starch polysaccharide compound enzyme at 0.5-1% After mixing and stirring evenly, ferment at 30-40°C for 24 hours to obtain a fermented mixed feed, add 5% lactic acid bacteria seed liquid to the fermented mixed feed, mix evenly and package it in an anaerobic fermentation device, and ferment for 48 hours. Get finished feed. The anti-nutritional factor degradation effect of the present invention is obvious, has fermented sour fragrance, good food-attracting effect, and has good production and application prospects.

Description

technical field [0001] The invention relates to a method for degrading feed anti-nutritional factors through bacterial enzyme synergistic fermentation, in particular to the degradation of anti-nutritive factors in corn and soybean meal-based diets. Background technique [0002] Corn soybean meal bran is currently the main feed raw material, and raw soybeans contain a large number of anti-nutritional factors, such as trypsin inhibitors, lectins, antigenic proteins, phytic acid and non-starch polysaccharides. The main antigenic proteins are macroglobulin and β-conglobulin. The bran contained in the diet also contains more non-starch polysaccharides. It is not conducive to the digestion and absorption of animals, especially young animals. [0003] Traditional physical processing methods such as heating and puffing can destroy a large number of heat-labile anti-nutritional factors such as lectins. However, the degradation of antigenic proteins, phytic acid, and non-starch pol...

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Problems solved by technology

[0004] However, the current bio-fermented feed mainly focuses on the fermentation of soybean meal, and the fermentation of compound feed is rare

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