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A kind of microbial preparation applied to aquaculture environment improvement and preparation method thereof

A microbial preparation and aquaculture technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of biological bactericides that have not been discovered yet, and achieve good production and application prospects, good decomposition effects, and preparation methods simple effect

Active Publication Date: 2017-10-20
福建汇盛生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, it is generally believed in the world that biological control may be one of the more effective ways to solve aquaculture diseases, but there is no biological fungicide specifically for preventing various diseases in aquaculture at home and abroad. Microbial agents for the prevention and treatment of aquaculture diseases are urgently desired by those skilled in the art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0045] And the specific operation steps of the microbial preparation preparation method are as follows:

[0046] (1) Cultivation of Rhodopseudomonas palustris strain PSB20: Take Rhodopseudomonas palustris strain PSB20 and inoculate it into medium Ⅰ, and culture it at 25-35°C and 2500-3500lx under light for 72-144h, When the OD of the culture medium 660 When the seed liquid reaches 1.5-2.2, the seed liquid is prepared; then the seed liquid is inoculated to transparent plastic agricultural film according to the inoculum amount of 5-20%, and cultivated at 30-35 °C for 3-5 days under sunlight, and the cloudy, And at night, use 60W / 25L incandescent lamp to irradiate, when the bacterial liquid turns deep red, the cultivation is completed;

[0047] (2) Concentration and solidification of Rhodopseudomonas palustris strain PSB20: add a flocculant to the cultured bacterial liquid obtained in step (1), let stand overnight to flocculate, and remove the supernatant; then add the protectiv...

Embodiment 1

[0055] Cultivation of strain PSB20: take strain PSB20 and inoculate it into medium Ⅰ, and culture it under light at 27°C and 2500lx for 72h, when the OD of the culture medium is 660 When the temperature reaches 1.5, the seed liquid is prepared; then, the seed liquid is inoculated to the transparent plastic agricultural film at 10% inoculation amount, at 30~35°C (when the temperature of the day is too high, spray water to cool down), and use sunlight to light Cultivate for 3 days, and irradiate with 60W / 25L incandescent lamp on cloudy days and nights. When the bacterial liquid turns deep red, the cultivation is completed.

[0056] Concentration and solidification of strain PSB20: Add aluminum potassium sulfate solution with a concentration of 0.5g / L to the cultured bacterial solution of strain PSB20, let it stand overnight for flocculation, and remove the supernatant; then add solid trehalose, diatomaceous earth ( crushing fineness 120 mesh), and then air-dried at 30°C to obtai...

Embodiment 2

[0061] Cultivation of strain PSB20: take strain PSB20 and inoculate it into medium Ⅰ, and culture it under light at 30°C and 3000lx for 96h, when the OD of the culture medium is 660 When the temperature reaches 1.7, the seed liquid is prepared; then inoculate the seed liquid to transparent plastic agricultural film according to the inoculation amount of 5%, at 30~35°C (when the temperature of the day is too high, spray water to cool down), and use sunlight to illuminate Cultivate for 5 days, and irradiate with 60W / 25L incandescent lamp on cloudy days and nights. When the bacterial liquid turns deep red, the cultivation is completed.

[0062] Concentration and solidification of strain PSB20: Add aluminum potassium sulfate solution with a concentration of 0.7g / L to the cultured bacterial solution of strain PSB20, let it stand overnight for flocculation, and remove the supernatant; then add solid trehalose and zeolite powder (crushed fineness 250 mesh), and then air-dried at 40°C...

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Abstract

The present invention screens and isolates a bacterial strain with the best effect of degrading nitrite from mud samples collected from ports such as Haicang and Tong'an in Xiamen City, Fujian Province, and screens and obtains the effect of degrading nitrite from mud flat samples in Ningde City, Fujian Province. Another best strain; the two strains are Rhodopseudomonas palustris PSB20 and Bacillus subtilis ND04 respectively; the microbial preparation prepared by using the two strains can degrade ammonia nitrogen and nitrous acid, and has a good decomposition effect on the residual organic matter of the bait, and can also inhibit the growth of Vibrio pathogenicum in the aquaculture water to a certain extent, that is, it has a good production and application prospect; in addition , the biological preparation also has the characteristics of a simple preparation method.

Description

technical field [0001] The invention belongs to the technical field of aquaculture water quality improvement, and in particular relates to a microbial preparation applied to aquaculture environment improvement and a preparation method thereof. Background technique [0002] With the development of the aquaculture industry, ponds are aging year by year, and the water quality and bottom quality of aquaculture are seriously polluted, resulting in frequent occurrence of aquaculture diseases. Water quality improvement has become one of the main measures for healthy aquaculture. [0003] In recent years, the research on water quality improvers is also in continuous development. Traditional water quality improvers use physical and chemical principles to improve water quality by absorbing or increasing dissolved oxygen in water. The efficiency is low, and it cannot meet the requirements of water purification in the current high-density farming mode. Most of them are biological water...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C12R1/125
CPCC02F3/34C02F2101/16C02F2101/166C12N1/20C12N1/205C12R2001/125C12R2001/38
Inventor 徐长安唐旭刘源森林凌方卫东黄仕新
Owner 福建汇盛生物科技有限公司
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