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Production method of acid soybean peptide protein

A soybean polypeptide and acidic technology, which is applied in the field of enzymatic and microbial collaborative production to produce acidic soybean polypeptide protein, can solve the problems of low lactic acid content and high cost of fresh soybeans

Active Publication Date: 2012-01-18
上海源耀农牧科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide an enzymatic method and microbial Cooperative production method for preparing acidic soybean polypeptide protein

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A method for synergistically producing acidic soybean polypeptide protein by enzymatic method and microorganism, which consists of the following steps:

[0019] (1) Take 100kg of commercially available peeled soybean meal, pulverize and sieve to obtain the crushed product of peeled soybean meal, add 3kg of glucose and 500kg of purified water, and then insert 2kg of Lactobacillus plantarum (2×10 8 cfu / mL) and 0.5kg Streptococcus thermophilus (3×10 8 cfu / mL), under anaerobic conditions, cultivated at 43°C until the pH of the fermentation broth dropped to 4.0 to obtain fermentation broth A;

[0020] (2) Pump fermentation broth A into the first enzymolysis tank, then heat up to 52°C, add 2kg of liquid acidic protease for enzymolysis for 40 hours, and obtain enzymolysis solution B after the enzymolysis reaction;

[0021] (3) The enzymolysis solution B is ultrafiltered with an ultrafiltration membrane with a molecular weight cut-off of 6000Da or less. Under the conditions of...

Embodiment 2

[0026] A method for synergistically producing acidic soybean polypeptide protein by enzymatic method and microorganism, which consists of the following steps:

[0027] (1) Take 100kg of commercially available peeled soybean meal, pulverize and sieve to obtain the crushed product of peeled soybean meal, add 1kg of glucose and 300kg of purified water, and then insert 1.5kg of Lactobacillus plantarum (2×10 8 cfu / mL) and 0.8kg Streptococcus thermophilus (3×10 8 cfu / mL), under anaerobic conditions, cultivated at 43°C until the pH of the fermentation broth dropped to 4.0 to obtain fermentation broth A;

[0028] (2) Pump fermentation broth A into the first enzymolysis tank, then heat up to 52°C, add 1.5kg of liquid acidic protease for enzymolysis for 50 hours, and obtain enzymolysis solution B after the enzymolysis reaction;

[0029] (3) The enzymolysis solution B is ultrafiltered with an ultrafiltration membrane with a molecular weight cut-off of 6000Da or less. Under the condition...

Embodiment 3

[0034] A method for synergistically producing acidic soybean polypeptide protein by enzymatic method and microorganism, which consists of the following steps:

[0035] (1) Take 100kg of commercially available peeled soybean meal, pulverize and sieve to obtain the crushed product of peeled soybean meal, add 2kg of glucose and 200kg of purified water, and then add 3kg of Lactobacillus plantarum (2×10 8 cfu / mL) and 3kg Streptococcus thermophilus (3×10 8 cfu / mL), under anaerobic conditions, cultivated at 43°C until the pH of the fermentation broth dropped to 3.8 to obtain fermentation broth A;

[0036] (2) Pump fermentation broth A into the first enzymolysis tank, then heat up to 52°C, add 2kg of liquid acidic protease for enzymolysis for 24 hours, and obtain enzymolysis solution B after the enzymolysis reaction;

[0037] (3) The enzymolysis solution B is ultrafiltered with an ultrafiltration membrane with a molecular weight cut-off of 6000Da or less. Under the conditions of a te...

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Abstract

The invention discloses a method for producing acid soybean peptide protein by using an enzyme method together with microorganisms. The method consists of the following steps of: (1) smashing and sieving commercially available peeled soybean meal, adding glucose and purified water, inoculating lactobacillus plantarum and streptococcus thermophiles, and culturing till the pH of a zymotic fluid is lowered to 4.4 to obtain a zymotic fluid A; (2) inoculating bifidobacteria into the zymotic fluid A and adding liquid acid protease for performing enzymolysis to obtain an enzymolysis solution A; and (3) sterilizing ultrafiltration permeate liquid obtained by performing ultrafiltration on the enzymolysis solution A, concentrating and drying to obtain a finished product, wherein the lactic acid content of the finished product is higher than 3.6 percent. In the method, peeled soybean meal which is much cheaper than the conventional generally-adopted soybean protein concentrate is taken as a raw material. Technically, enzymolysis is performed after lactobacillus plantarum and streptococcus thermophiles are used of lowering the pH of the entire enzymolysis system to pH which is most suitable for the acid protease; and meanwhile, a part of soluble non-starch polysaccharides in the peeled soybean meal can be degraded in the growing processes of the lactobacillus plantarum and the streptococcus thermophiles, and the finished product also contains organic acid substances.

Description

technical field [0001] The invention relates to the technical field of deep processing of soybean protein, in particular to a method for producing acidic soybean polypeptide protein through the synergistic production of enzyme method and microorganism. The soybean polypeptide produced by the method can be used as a nutritional enhancer and a feed additive for health food. Background technique [0002] Soybean protein is currently the most abundant plant protein raw material in the world. It is rich in protein and a variety of physiologically active ingredients. 70% of the plant protein ingested by humans comes from soybean protein. Soy protein has amino acid balance, rich nutrition, and easy absorption. characteristic. Soybean polypeptide is a protein hydrolyzate obtained from soybean protein after the action of protease and then after special treatment. It has the same essential amino acid composition as soybean protein, balanced and rich content, and less antigenicity. Ma...

Claims

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Application Information

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IPC IPC(8): A23J1/14A23K1/14A23K10/12A23K10/14A23K20/147
Inventor 方华李旺军季春源
Owner 上海源耀农牧科技有限公司
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