20 results about "Lipase preparation" patented technology

The invention relates to the technical field of grease chemistry, in particular to a preparation method and application of diglyceride oil capable of preventing and treating fatty liver. The preparation method comprises the following steps: (1) mixing raw material oil, water, glycerol and a lipase preparation for catalytic reaction to obtain a product 1; the raw material oil comprises 0.1-8 parts of sea buckthorn seed oil, 0.1-8 parts of acer truncatum seed oil, 60-90 parts of low erucic acid rapeseed oil, 15-35 parts of corn oil and 10-25 parts of peanut oil; the lipase preparation is aspergillus niger lipase and rhizopus niger lipase; (2) performing five-stage molecular distillation on the product 1 to obtain a product 2; and (3) decoloring and deodorizing the product 2 to obtain the product. The diglyceride oil prepared by the invention has excellent effects of preventing and treating fatty liver.

The present invention discloses a method and application for simultaneously reducing polycyclic aromatic hydrocarbons in tobacco leaves and cigarette smoke, as well as a cigarette, which relates to the technical field of harm reduction in tobacco processing. The method includes: preparing a lipase solution; uniformly spraying the lipase solution on both the front and back sides of the tobacco leaves to be treated; subjecting the tobacco leaves sprayed with the lipase solution to constant temperature and humidity treatment, and the content of polycyclic aromatic hydrocarbons in the tobacco leaves after lipase treatment is reduced; cutting the tobacco leaves treated with lipase into shreds, and inactivating the lipase in the shredded tobacco leaves treated with lipase; rolling the inactivated shredded tobacco into cigarette sticks, and the content of polycyclic aromatic hydrocarbons in the smoke after combustion is reduced. The method is applicable to low-grade flue-cured tobacco or cut tobacco after threshing and redrying. The cigarette includes shredded tobacco obtained by lipase treatment. The method of the present invention is simple and easy to operate. After treatment with lipase preparation, it can specifically reduce the content of PAHs in tobacco leaves and cigarette smoke, especially the content of fluoranthene, and can significantly improve the safety of tobacco with a high fluoranthene content.

Lipase compositions and formulations, as well as methods of treatment and manufacture, are disclosed.

The invention discloses a preparation method of immobilized lipase and application of the immobilized lipase in improvement of catalytic activity and / or stability of the immobilized lipase, the preparation method comprises the following steps: carrying out a coordination reaction on a lipase solution, a zinc salt and an organic ligand under a weakly alkaline condition to obtain the immobilized lipase, the lipase solution is a propanol solution containing lipase. According to the method, one-step synthesis of the lipase (at) MOF biological composite material is realized through a direct aggregation conformation locking strategy, and compared with a traditional solvothermal method, a spray layer-by-layer liquid phase epitaxy method and a phase change method, the method is simpler and more convenient to operate, crystallization is performed under mild conditions, lipase is immobilized, the influence on lipase is smaller, and the method is suitable for industrial production. The lipase has strong biological catalytic activity and excellent stability which are obviously superior to those of natural free lipase, can be recycled for multiple times, and has important popularization and application values in large-scale utilization of lipase in industrial production in biological catalytic reaction.

The invention belongs to the technical field of laundry detergents, and particularly relates to a phosphorus-free laundry detergent based on an enzyme preparation and a preparation method of the phosphorus-free laundry detergent. The non-phosphorus laundry detergent based on the enzyme preparation is prepared from the following components in percentage by mass: 10 to 15 percent of anionic surfactant, 4 to 8 percent of nonionic surfactant, 1 to 3 percent of zwitterionic surfactant, 0.1 to 0.5 percent of papain preparation, 0.1 to 0.5 percent of lipase preparation, 1 to 3 percent of propylene glycol, 2 to 4 percent of trisodium citrate, 0.02 to 0.04 percent of polyether defoaming agent, 0.10 to 0.15 percent of citric acid and the balance of water. The laundry detergent prepared by the invention is strong in dirt-removing power and high in storage stability.

This invention discloses a method for preparing millet lipase, belonging to the fields of bioengineering and deep processing of agricultural products. The method uses millet flour as raw material, extracts a crude enzyme solution using a buffer solution, and further constructs a polyethylene glycol-inorganic salt aqueous two-phase system to achieve targeted distribution of the millet lipase, completing rapid enrichment and preliminary purification. Subsequently, polyethylene glycol and inorganic salts are removed from the system by ultrafiltration or dialysis, and molecular sieve chromatography is used for fine purification when necessary to obtain a high specific activity millet lipase preparation. Experimental results show that compared with the traditional ammonium sulfate fractionation precipitation method, the method of this invention significantly shortens the processing time, improves the recovery rate and purification fold of the lipase, and has mild operating conditions and good reproducibility, demonstrating good potential for industrial application.

The invention belongs to the technical field of enzyme preparations, and particularly provides an oil-soluble lipase preparation which comprises lipase powder and an emulsifier in a mass ratio of 1000: (6.5-46), the lipase powder comprises lipase and a stabilizer. The invention also provides a preparation method of the oil-soluble lipase preparation, and the oil-soluble lipase preparation prepared by the method provided by the invention has a good effect of emulsifying an oil-water system, and can promote lipolysis and greatly improve the utilization rate of lipase.

The application belongs to the technical field of oil and fat chemistry, and particularly relates to a diglyceride oil capable of preventing fatty liver, a preparation method and application of the diglyceride oil, and the preparation method comprises the following steps: (1) mixing raw oil with glycerol, adding a lipase preparation, and performing enzymatic reaction in a solvent-free system; the content of monoglyceride in the raw oil is 40-80 wt%; (2) separating the reaction product of step (1), collecting the upper oil phase, and then performing secondary molecular distillation purification on the oil phase to obtain an oil product with a diglyceride content of greater than or equal to 80 wt%. The technical scheme of the application uses raw oil with a monoglyceride component content greater than 40% as raw material to synthesize an oil product with a high diglyceride content, and the amount of the lipase preparation added is low, so that the production cost can be effectively reduced, and the obtained product has high diglyceride and sterol contents and has a significant effect in preventing fatty liver.

The invention belongs to the technical field of biological enzymes, and particularly relates to a preparation method of immobilized lipase and application of the immobilized lipase in catalysis of oil hydrolysis, and the preparation method comprises the following steps: step 1, material selection: selecting Candida antarctica lipase A, porous ceramic and a phosphate buffer; according to the preparation method of the immobilized lipase and the application of the immobilized lipase in catalysis of grease hydrolysis, porous ceramic is adopted as an immobilization carrier, and candida antarctica lipase A is immobilized; the obtained immobilized lipase is used for catalyzing a grease hydrolysis reaction, and the acid value of a product obtained after the grease hydrolysis reaction is catalyzed can reach 180-195 mgKOH / g. Compared with free enzyme, the immobilized enzyme has the advantages that the catalytic activity of the enzyme is obviously improved under the same condition; in addition, the immobilized enzyme has high thermal stability and pH tolerance; the immobilized enzyme is good in reusability and convenient to recycle.

The invention discloses a double-bacterium synergistic fermentation enzyme modified cheese flavoring agent and a preparation method thereof, and the double-bacterium synergistic fermentation enzyme modified cheese flavoring agent is prepared from the following raw materials in parts by mass: 10-20 parts of milk powder, 40-50 parts of anhydrous butter, 0.5-1.5 parts of an emulsifier, 0.1-1.0 part of a protease preparation, 0.1-1.0 part of a lipase preparation, 5-15 parts of a microbial fermentation agent and the balance of water, the microbial fermentation agent is composed of lactobacillus helveticus and saccharomycetes, and the saccharomycetes comprise one of kluyveromyces marxianus, kluyveromyces lactis and candida lactis. Ethyl ester compounds are dynamically generated through esterification reaction of ethyl alcohol generated through yeast metabolism and carboxylic acid synthesized by lactic acid bacteria, the natural cheese characteristic flavor with rich levels is formed, and the obtained final product is soft in flavor, free of irritation and rich and natural in fragrance.

The present disclosure provides a method for rapid and harmless treatment of a livestock and poultry carcass / residue by a bacterium-enzyme complex and relates to the technical field of dead livestock and poultry treatment. In the present disclosure, a composite microbial inoculant is compounded from protease-, lipase-, and keratinase-producing strains, and can be applied to the degradation of fats and proteins as well as deodorization. The composite microbial inoculant is further combined with a protease preparation and a lipase preparation to prepare the bacterium-enzyme complex. The bacterium-enzyme complex can quickly and harmlessly treat the carcasses / residues of pig, cattle, sheep, and chicken, and has a short treatment period, mild and controllable reaction conditions, and safe and non-toxic reaction process and reaction products. During the reaction, harmful gases such as CH4 and NH3 and greenhouse gases such as CO2 released by the method are less than those by other methods.

The invention relates to the technical field of grease chemistry, in particular to diglyceride oil capable of preventing fatty liver and a preparation method and application thereof.The preparation method of the diglyceride oil comprises the following steps that 1, raw material oil and glycerinum are mixed, a lipase preparation is added, and an enzymolysis reaction is conducted in a solvent-free system; the content of monoglyceride in the raw oil is 40-80 wt%; and (2) separating the reaction product in the step (1), collecting an upper-layer oil phase, and then carrying out secondary molecular distillation purification on the oil phase to obtain a grease product with the diglyceride content of more than or equal to 80wt%. According to the technical scheme, the raw material oil with the monoglyceride component content larger than 40% is adopted as the raw material to synthesize the oil product with the high diglyceride content, the addition amount of the lipase preparation is low, the production cost can be effectively reduced, the diglyceride content and the sterol content in the obtained product are high, and the obvious effect is achieved in the aspect of preventing the fatty liver.

The invention belongs to the technical field of biological catalysis and enzyme engineering, and particularly relates to a preparation method of covalent immobilized lipase based on amino functionalization MOF (Metal Organic Framework) and application of the covalent immobilized lipase in synthesis of diglyceride. The invention discloses a method for cross-linking and immobilizing CALB lipase on a zirconium-based metal organic framework NH2-UiO-66 by adopting glutaraldehyde as a cross-linking agent, the obtained immobilized lipase is defined as CALB-coated GA-NH2-UiO-66, and the immobilized lipase is applied to catalytic esterification synthesis of diglyceride. The oleic acid conversion rate is determined by adopting an acid value, and the composition of a product is analyzed by HPLC-ELSD. The immobilized lipase CALB (at) GA-NH2-UiO-66 disclosed by the invention has the advantages of high stability, reusability, high catalytic efficiency and diglyceride selectivity. The high DAG production significantly mitigates downstream purge burden. A promising solution is provided for industrial production of DAG, and a deep insight is provided for a fixing mechanism of a functionalized metal-organic framework.

The invention belongs to the technical field of biological catalysis, and particularly relates to a preparation method of immobilized lipase and application of the immobilized lipase in catalytic synthesis of glyceride, the preparation method comprises the following steps: (1) surface modification: immersing a pretreated carrier in 80-300 parts by weight of a silanization solution for reaction to obtain functional resin; (2) enzyme immobilization: putting the functionalized resin into an enzyme solution, and oscillating and adsorbing to form an enzyme-resin compound; and (3) post-treatment: washing the enzyme-resin compound with 300-600 parts by weight of a phosphate buffer solution, and drying until the water content is less than or equal to 5% to obtain a finished product of the immobilized lipase. The immobilized lipase is used for catalytic synthesis of glyceride, and the glyceride comprises diglyceride and triglyceride. The enzyme stability is improved by combining covalent modification on the surface of the carrier with a protective auxiliary agent, the composition of a glyceride product is accurately regulated and controlled by utilizing a temperature-vacuum synergistic mechanism, and the reaction kinetic performance is enhanced by virtue of a composite dewatering system.

The invention relates to a preparation method of lipase for preparing diester oil. The preparation method is characterized by comprising the following steps: (1) preparing a lipase solution, namely performing high-density fermentation on a lipase strain; (2) preparing the immobilized lipase: mixing the lipase liquid obtained by high-density fermentation with a resin carrier, heating and adsorbing in a shaking table, immobilizing for a certain time, cleaning with pure water, filtering, and drying to obtain the immobilized lipase; (3) catalytic reaction: mixing edible oil, glycerol and immobilized lipase, and heating to react for a certain time to prepare crude diester oil; (4) purifying the crude diester oil by an ethanol multiple extraction temperature control method, and removing fatty acid and monoglyceride; and carrying out vacuum drying to remove ethanol and water to obtain the diester oil with high diglyceride content. The invention provides a brand-new purification method, namely an ethanol extraction method, for purifying diglyceride oil by improving the stability and the use frequency of enzyme through self-made immobilized lipase. Compared with a traditional molecular distillation method, the method is higher in efficiency and mainly has the advantages that purification equipment is simple and easy to obtain, low-temperature conditions are adopted in the whole process, the single purification material amount is large, and the extraction time is short.

The application discloses a preparation method of immobilized lipase and application of the immobilized lipase in catalysis of 1,3-glycerol diglyceride synthesis. The preparation method comprises the following steps: (1) preparation of a template; (2) immobilization of lipase; and (3) removal of the template. The prepared immobilized enzyme is used for catalyzing conversion of TAG of vegetable oil into DAG, and the selectivity of 1,3-DAG can be remarkably improved, and the proportion of 1,3-DAG in the product is 80% to 90%.

The present invention relates to the technical field of oil and fat chemistry, and specifically to a preparation method and application of diglyceride oil capable of preventing and treating fatty liver. The preparation method comprises the following steps: (1) mixing raw material oil, water, glycerol and lipase preparation for catalytic reaction to obtain product 1; the raw material oil comprises 0.1-8 parts of seabuckthorn seed oil, 0.1-8 parts of maple seed oil, 60-90 parts of low-erucic acid rapeseed oil, 15-35 parts of corn oil and 10-25 parts of peanut oil; the lipase preparation is Aspergillus niger lipase and Rhizopus niveus lipase; (2) subjecting product 1 to five-stage molecular distillation to obtain product 2; (3) subjecting product 2 to decolorization and deodorization, that is, obtaining. The diglyceride oil prepared by the present invention has excellent effect in preventing and treating fatty liver.

The present invention belongs to the field of bioengineering technology and relates to a lipase, a gene encoding the lipase, a recombinant expression vector and a recombinant expression transformant containing the lipase gene, a method for preparing the lipase, and a method for preparing methyl epoxy stearate using the lipase. Compared with the prior art, the lipase of the present invention has high activity and good stability. The enzyme is used to catalyze hydrogen peroxide and n-heptanoic acid to form peroxycarboxylic acid, which is then reacted with methyl oleate to generate methyl epoxy stearate. This avoids the large demand for strong acids and metal catalysts and the complex reaction apparatus in conventional chemical catalysis methods. The process is simple, the process is environmentally friendly, and there is good prospect in the industrial production of methyl epoxy stearate.

The invention discloses a method for simultaneously reducing polycyclic aromatic hydrocarbon in tobacco leaves and smoke, application and cigarettes, and relates to the technical field of tobacco processing harm reduction. The method comprises the following steps: preparing a lipase solution; uniformly spraying the lipase solution on the front and back surfaces of the to-be-treated tobacco leaves; the tobacco leaves sprayed with the lipase solution are subjected to constant-temperature and constant-humidity treatment, and the polycyclic aromatic hydrocarbon content of the tobacco leaves subjected to lipase treatment is reduced; shredding the tobacco leaves treated by the lipase, and inactivating the tobacco shreds treated by the lipase by the lipase; the inactivated tobacco shreds are rolled into cigarettes, and the content of polycyclic aromatic hydrocarbon in smoke after the cigarettes are burnt is reduced. The method is suitable for low-grade flue-cured tobacco or threshed and redried tobacco lamina. The cigarette comprises tobacco shreds obtained through lipase treatment. The method is simple and easy to operate, the content of PAHs, especially the content of fluoranthene, in tobacco leaves and smoke can be pertinently reduced after treatment of the lipase preparation, and the safety of the tobacco with the high content of fluoranthene can be remarkably improved.

The invention discloses a preparation method of biomass diesel oil immobilized lipase, and belongs to the technical field of lipase immobilization. The preparation method specifically comprises the following steps: (1) drying polystyrene resin, and adding methanol for swelling, suction filtration, washing and drying to obtain pretreated resin; (2) adding L-lysine, L-arginine, L-histidine or L-cysteine into the enzyme solution of the biomass diesel lipase, and stirring for dissolving, so as to obtain a pretreated enzyme solution; and (3) adding the pretreated enzyme liquid into the pretreated resin for adsorption, suction filtration, washing and drying. According to the biomass diesel oil immobilized lipase prepared by the invention, the content of free FFA in a biomass diesel oil reaction can be reduced to 0.5% or below, the cycle use frequency exceeds 14 times, the biomass diesel oil immobilized lipase has the characteristics of simple preparation process, excellent catalytic performance, greatly improved stability and the like, and the production cost is greatly reduced.