64 results about "Antifungal protein" patented technology

An object of the present invention is to search and identify novel antifungal proteins capable of inhibiting the growth of plant pathogenic microorganisms including Magnaporthe grisea and Rhizoctonia solani causing two major rice diseases at relatively low concentrations, and further to clone a gene for said protein. The present invention provides an antifungal protein which can be obtained from fraction(s) precipitated by ammonium sulfate precipitation using an aqueous extract from Pleurotus cornucopiae, wherein said protein has an antifungal activity against at least rice blast, and exhibits existence of a component having a molecular weight of about 15 kDa as determined by SDS-PAGE method; a gene encoding said protein and uses thereof.

The present invention relates to an antifungal protein, AlfAFP1, which is a modified form of an antifungal protein isolated from Medicago plants, the modified form exhibiting enhanced anti-fungal activity for controlling fungal pathogenesis in plants. A method for inhibiting fungal colonization of plants is described which includes preparation of nucleotide sequences encoding the modified antifungal protein, preparation of vectors containing the nucleotide coding sequence, and methods for transforming plants with the nucleotide sequences. The polypeptide can be formulated into compositions useful in controlling plant pathogenic fungi.

The invention relates to an antifungal protein separated and purified from bacillus subtilis J18, a protein product, a preparation method and an application. A strain serial number of the bacillus subtilis J18 is CGMCC No.3665, and a strain name is the bacillus substilis J18. The preparation method of the protein product comprises the following steps of: mixing the J18 antifungal protein with 1 percent of acetic acid to form a chitosan solution with a pH value of 6.0, compositing the chitosan solution to form a film coating reagent which has the concentration of 0.05mg / mL and is used for soaking a fruit, and also coating a mixture which is formed by mixing the J18 antifungal protein solution with an oxidized starch solution on a preservative paper to prepare an antifungal preservative paper used for wrapping the fruit. The antifungal protein has remarkable control effect to fruit and crop diseases and particularly black spot diseases of pears after picking and is an antagonistic strain with broad spectrum and high efficiency.

A method of treating or preventing fungal infection, which includes identifying a plant or animal having a fungal infection and administering an effective amount of an anti-fungal composition to the plant or animal to reduce the fungal infection. In a preferred form of the present invention, the antifungal composition is compound 13 or 33 and is combined with an azole compound, such as fluconazole.

The invention specifically discloses a microbial inoculant, in particular to a microbial inoculant prepared from swine borne bacillus licheniformis YHSH-02 and the application thereof in livestock breeding. The culture is preserved in CGMCC, 3#, Yard #1, West Beichen Road, Chaoyang District, Beijing, with the preservation number of CGMCC NO. 15454 and the preservation date of 15th, March, 2018. The bacillus licheniformis YHSH-02 is separated from a swine intestinal tract, can generate rich lipopeptides bacilli, antifungal proteins and the like, and can inhibit escherichia coli, pseudomonas aeruginosa, enterococcus faecalis, enterobacter cloacae, pseudomonas aeruginosa, proteusbacillus vulgaris, staphylococcus aureus, salmonella and clostridium. The bacillus licheniformis YHSH-02 obtained from the swine intestinal tract is a biological control potential culture which is high in preventing and treating efficiency and wide in preventing and treating range and can promote growth obviously,and has a good application and development prospect in non-resistant breeding.

The invention discloses a coral-associated marine bacillus amyloliquefaciens strain CoMb-9 and an application thereof. The strain is preserved in the Guangdong Microbiological Culture Collection Center on Floor 5, Building 59, Courtyard 100, Xianlie Middle Road, Guangzhou, Guangdong Province on April 19, 2016, and the preservation number is GDMCC No. 60028. The strain has strong effects of inhibiting growth of plant phytophthora bacteria, plant wilt pathogenic bacteria and / or plant colletotrichum orbiculare and the like, can be colonized to propagate in soybeans, hot peppers, bananas and other crops or in rhizosphere soil of the above crops, secretes to produce antifungal proteins, indoleacetic acid and other active materials, has the effects of promoting crop growth and preventing and treating multiple plant diseases, can be applied to developing and preparing bio-organic fertilizers, biological disease prevention preparations and other agricultural biological agents with effects of promoting crop growth, improving crop yield and preventing and treating the multiple plant diseases, and has excellent popularization and application prospects in related agricultural production.

The invention discloses a eucommia fatigue-relieving health care beverage and a preparation method thereof, and belongs to the field of beverages in the food processing industry. The beverage is characterized by being composed of the following raw materials in parts by weight: 60 to 70 parts of eucommia extract, 5 to 10 parts of honey, and 25 to 30 parts of purified water. The beverage is prepared by mixing the abovementioned raw materials, homogenizing the mixture and sterilizing the mixture at a high temperature. The eucommia beverage is fragrant, odorless, nonirritant, clear, and transparent, has a proper taste, does not contain any impurity, precipitate or floating dregs, and does not become layering or turbid. Because eucommia leaf contains fungus resistant proteins, the produced green additive-free beverage has the functions of relieving fatigue, strengthening the muscle and bones, enhancing the immunity, reducing blood fat and blood pressure, preventing bacteria, and eliminating inflammation. The taste of the beverage is like fruit juice. The health care needs of most people can be met by the beverage. The beverage can be drunk for a long term and has characteristics in appearance, color, and taste.

Transgenic plants, plant tissue, and propagation materials are disclosed that exhibit or convey increased resistance to pathogens of multiple higher-order phylogenetic lineages. The disclosed transgenic plants and plant tissues include plant cells containing a DNA construct encoding Gastrodia Anti-Fungal Protein (GAFP), also known as gastrodianin, an anti-fungal gene naturally occurring in a Chinese orchid, Gastrodia elata. Transgenic plants disclosed include herbaceous plants as well as woody plants, including fruit trees. Disclosed transgenic plants can also be beneficially utilized as rootstock, for instance rootstock for stone fruit crops such as peach, thereby conferring enhanced disease resistance to the rootstock without genetically altering the scion.

The invention provides marine penicillium citrinum antifungal protein PcPAF and a preparation method thereof, and relates to microbial original antifungal protein. Marine penicillium citrinum W1 is stored as CCTCC (China Center for Type Culture Collection) NO: M2013205. According to the preparation method, supernatant of fermentation broth of marine penicillium citrinum W1 is separated and purified to obtain antifungal protein, naming PcPAF; the identification result shows that the obtained mass spectrometry information is failed to be matched in NCBI (National Center of Biotechnology Information) Genbank database; 15 amino acids at the N end of the protein are detected by an N end sequencing manner; by being compared with the NCBI Genbank database, the obtained amino acid sequence shows low homology, so that PcPAF can be determined to be a novel antifungal protein. By the N end sequencing, the 15 amino acids at the N end are AGNRPDFPRRHHPGG. PcPAF shows obvious effect on inhibiting pathomycete, namely, trichoderma viride and the like, of four plants, and remains the antimicrobial activity after being heated for 20 minutes at 80 DEG C.

The invention relates to the field of molecular biology, in particular to a method for improving the resistance of wheat to sharp eyespot by adopting a genetic engineering method to introduce a rhizoma gastrodiae antifungal protein gene and a rabbit defensin gene into conventional wheat and expressing. The method for improving resistance to wheat sharp eyespot comprises the following steps: constructing an expression vector pAHC-NP-GAFP of the rhizoma gastrodiae antifungal protein gene and the rabbit defensin gene; introducing the pAHC-NP-GAFP into receptor-wheat immature embryo cells through particle bombardment, performing callus induction and plant regeneration on the wheat immature embryo cells to obtain transgenic candidate plants, obtaining positive transgenic plants through molecular detection and obtaining T5-generation transgenic homozygous lines from the positive transgenic plants through inbreeding and PCR screening; performing sharp eyespot resistance screening on progenies of the T5-generation transgenic homozygous lines; and screening out the transgenic plant with improved resistance to sharp eyespot. The method has the advantages that the method has the characteristics of strong pertinence, short cultivation period and little influence on important agronomic characters compared with conventional planting-cultivating methods.

The invention discloses yersinia pseudotuberculosis-sourced antifungal protein and a coding gene and application thereof, and belongs to the technical field of genetic engineering. The antifungal protein is protein comprising the amino acid sequence shown as SEQ ID NO: 1, or protein obtained by substituting, deleting or inserting one or several amino acid sequences in the amino acid sequence shownas SEQ ID NO: 1 and having equivalent activity. The invention further provides the coding gene, an expression vector and engineering bacteria of the antifungal protein. The invention further providesa separation and purification method of the yersinia pseudotuberculosis-sourced antifungal protein. The protein is applied to an antifungal agent and the coding gene of the protein is applied to a transformed plant. The YPK_AFP1001 antifungal gene and the protein, related by the invention, have higher and more broad-spectrum antifungal activity.

The invention discloses a nucleic acid sequence of a pteromalus puparum antifungal protein Pp-AP4 code. The separated DNA molecule comprises: a polypeptide nucleotide sequence with the pteromalus puparum antifungal protein Pp-AP5 activity is encoded, the nucleotide sequence and the nucleotide sequence from the 226th ribonucleotide to the 345th ribonucleotide in the SEQ ID NO:1 have 70 percent of homology; or the nucleotide sequence can be hybridized with the nucleotide sequence from the 226th ribonucleotide to the 345th ribonucleotide in the SEQ ID NO:1 at a temperature of between 40 and 55 DEG C. The invention also discloses a pteromalus puparum antifungal protein polypeptide Pp-AP4 which has an amino acid sequence shown in the SEQ ID NO: 2. The pteromalus puparum antifungal protein Pp-AP4 has the antibiosis performance.

Disclosed are a novel strain with antibacterial activity, identified as Streptomyces sp. AN1542 strain (Accession No.: KCTC 12113BP), an antibacterial composition comprising a compound represented by Chemical Formula 1 or 2, or a strain producing the same as an active ingredient, and a method for producing the same. Exhibiting potent antibacterial activity against pathogenic microbes and antibiotic-resistant microbes, particularly, MRSA, QRSA, VRSA and VRE, the compound or the strain of the present invention can be applied to the treatment of infectious diseases caused by superbacteria.

The present invention belongs to the field of gene engineering technology, and especially a kind of active promoter segment F1 of Bacillus pumilus, its fusion gene constructing body and new recombinant expression vector carrying the constructing body. The present invention also relates to the said random alveolus mutation, antifungal protein acquisition, expression vector transformed Bacillus pumilus cell and transformed cell generated antifungal function. The said promoter activity improvement can resist chloromycetin as high as 900 mcg / ml, and the obtained antifungal protein secretion obtained under the directing of the promoter has certain antifungal characteristic.

The present invention belongs to the field of gene engineering technology, and especially a kind of active promoter segment F1 of Bacillus pumilus, its fusion gene constructing body and new recombinant expression vector carrying the constructing body. The present invention also relates to the said random alveolus mutation, antifungal protein acquisition, expression vector transformed Bacillus pumilus cell and transformed cell generated antifungal function. The said promoter activity improvement can resist chloromycetin as high as 900 mcg / ml, and the obtained antifungal protein secretion obtained under the directing of the promoter has certain antifungal characteristic.

The invention belongs to the technical field of genetic engineering, and in particular relates to a method for improving the antibacterial activity of antifungal protein. The method comprises the following steps of: connecting a DNA (deoxyribonucleic acid) sequence of a coding chitin binding domain with a cDNA (complementary DNA) sequence of coded antifungal protein by a correct reading frame; then cloning to a protein expression vector; expressing fused protein by using engineering bacteria; carrying out protein purification by further utilizing a commercialized medium; and detecting the chitin binding capacity of the purified protein and analyzing the antibacterial activity. The invention can improve the antibacterial efficiency of the antifungal protein. By applying the invention, the activity of the traditional antifungal protein and the targeted binding capacity of the protein on fungal cell walls are improved.

The invention discloses an application of a domestic silkworm cocoon antifungal protease inhibitor in fungus prevention and a separation method of the domestic silkworm cocoon antifungal protease inhibitor. The separation method comprises the following steps: layering and cutting a silkworm cocoon into pieces, then adding 100mm of Tris-HCL buffer solution with pH of 7.5, and extracting for 30 minutes under the condition that the temperature is 37 DEG C and the rotation speed is 220 r / pm, centrifuging for 15 minutes under the rotation speed of 10000rpm, filtering supernatant by utilizing a 0.22-micrometer filter membrane, to obtain the domestic silkworm cocoon antifungal protease inhibitor. The prepared protease inhibitor can inhibit the activity of fungal protease secreted by Tritirachium album limber, can inhibit the spore germination of silkworm pathogenic fungal beauveria bassiana and still can maintain the activity after being incubated for 10 minutes in boiled water. The prepared domestic silkworm cocoon antifungal protease inhibitor belongs to the natural, high-activity and high-stability antifungal protease and is non-toxic and harmless; and meanwhile, the silk used in the method is rich in resource, and a novel way is provided for diversified development of the silk.

The invention discloses a nourishing chafing dish soup base, which is composed of fresh black-bone chicken soup 1,500 g, Alisma orientale 80-120 g, Panax ginseng 40-60 g, Gastrodia elata 40-60 g, cassia bark 8-12 g, star aniseed 20-30 g, cumin 20-30 g, dried chili powder 25-35 g, pricklyash peel 10-20 g, clove 25-35 g, fermented glutinous rice liquid 40-60 g, and a proper amount of table salt. The black-bone chicken is rich in vitamins, trace elements and amino acids, and is a domestic fowl with high nutritive value; Alisma orientale has the effects of promoting diuresis, resolving phlegm, reducing pathogenic fire, removing moist heat, replenishing vital essence, and invigorating qi; Panax ginseng has the effects of invigorating qi, tonifying blood, refreshing and caring skin and is a precious Chinese medicinal material with slight sweetness; and Gastrodia elata contains pharmacological active substances such as gastrodine and vanillin, as well as antifungal proteins, and amino acids and trace elements needed by human body. The chafing dish soup produced with the inventive chafing dish soup base has good taste and high nutritive value, and low liability to suffer from excessive internal heat.

Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include amino acid sequences, and variants and fragments thereof, for novel variants of antipathogenic polypeptides generated through DNA shuffling that exhibit improved antipathogenic activity. Polynucleotides that encode the antipathogenic polypeptides are also provided. A method for inducing pathogen resistance in a plant using the polynucleotides disclosed herein is further provided. Compositions comprising an antipathogenic polypeptide or a microorganism comprising an antipathogenic polynucleotide of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Plants, plant cells, seeds, and microorganisms comprising an antipathogenic polynucleotide or polypeptide of the invention are also disclosed.

The invention discloses a strain of bacillus amyloliquefaciens, and an antifungal protein and a prawn fresh-keeping application thereof. The bacillus amyloliquefaciens PJ has been preserved in Guangdong Culture Collection Center (GDMCC) on September 12nd, 2016. The address of GDMCC is fifth floor, No.59 building, courtyard, Xianlie middle road No.100, Guangzhou, Guangdong province. The preservation number is GDMCC No. 60076. The provided novel strain and antifungal protein thereof have a biological activity on preventing pseudomonas, micrococcus luteus, shewanella spp., and acinetobacter, can be applied to the fields such as health care product, food fresh-keeping, cosmetic preservative, and the like, and have a good market potential.

The present invention relates to an antifungal protein gene and cDNA sequence thereof, which is obtained by mining the whole genome sequences of Monascus pilosus BCRC 38072 and the unigene database. The gene can encode an antifungal protein MAFP1. A purified protein obtained from M. pilosus culture broth having molecular weight of about 7 kDa is identified as MAFP1 by N-terminal protein sequencing and comparative analysis. The purified MAFP1 protein can inhibit the growth of pathogens such as Paecilomyces variotii BCRC 33174 and Helminthosporium panici BCRC 35004. In addition, it is found by PCR test that the gene of this antifungal protein exists in other Monascus species such as M. Barkeri, M. floridanus, M. lunisporas, M. pilosus, M. ruber and the like. It is also been proved that the mafp1 gene and cDNA thereof in four Monascus strains, M. pilosus (BCRC 38072, BCRC 38093 and BCRC 31502) and M. ruber BCRC 31533, have the same DNA sequences.

The invention provides an antifungal protein, which is obtained by purifying a water soluble extract of hulless oat seed, wherein the apparent molecular weight of the antifungal protein is about 35kDa. The preparation method comprises the following steps: crushing and degreasing the hulless oat seed; extracting the hulless oat seed by buffer solution to obtain a coarse protein; and obtaining the antifungal protein by the two-step chromatographic separation which is the Resource S cation exchange and Superdex 75 gel exclusion chromatographic purification. The antifungal protein has obvious effect of inhibiting the growth of white rot fungus and trichoderma viride, and the minimum inhibition for the white rot fungus is 0.42mu g, and 0.21mu g for the thetrichoderma viride. In addition, the antifungal protein also has certain effect of inhibiting the growth of fusarium. The antifungal protein can be used as a food anticorrosion additive, a crop antifungal damage spraying agent and the like, and has high practical values in the aspects of food anticorrosion, crop prophylaxis and crop disease resistance.

The invention belongs to the technical field of antifungal drug combination, and particularly relates to application of combination of D-penicillamine and fluconazole drug in preparing antifungal products. Candida albicans is an infectious fungus with great harm degree, and the clinical common antifungal drug variety is limited. Drug-resistant strains continue to appear with the progress of treatment. The invention provides a study on the application of the combination of the D-penicillamine and the fluconazole antifungal drug in the treatment of drug-resistant candida albicans. The study result shows that the D-penicillamine can obviously reduce the dosage of the fluconazole medicament, reduce the drug resistance probability of the strain and reduce the dosage of the medicament, thereby being beneficial to eliminating the toxic and side effects caused by accumulation of the medicament in vivo. In addition, the combination of the D-penicillamine and the fluconazole drug has selective inhibition effect on drug-resistant strains, and also provides research basis for overcoming the drug resistance of fungi.

An object of the present invention is to search and identify novel antifungal proteins capable of inhibiting the growth of plant pathogenic microorganisms including Magnaporthe grisea and Rhizoctonia solani causing two major rice diseases at relatively low concentrations, and further to clone a gene for said protein. The present invention provides an antifungal protein which can be obtained from fraction(s) precipitated by ammonium sulfate precipitation using an aqueous extract from Pleurotus cornucopiae, wherein said protein has an antifungal activity against at least rice blast, and exhibits existence of a component having a molecular weight of about 15 kDa as determined by SDS-PAGE method; a gene encoding said protein and uses thereof.

Provided are transgenic plants expressing MtDef5 antifungal proteins and peptides exhibiting high levels of resistance to susceptible fungi. Such transgenic plants contain a recombinant DNA construct comprising a natural or heterologous signal peptide sequence operably linked to a nucleic acid sequence encoding these molecules. Also provided are methods of producing such plants, methods of protecting plants against susceptible fungal infection and damage, as well as compositions that can be applied to the locus of plants, comprising microorganisms expressing these molecules, or these molecules themselves, as well as pharmaceutical compositions containing these molecules. Human and veterinary therapeutic use of MtDef5 antifungal proteins and peptides to treat susceptible fungal infections are also encompassed by the invention.

The invention discloses an application of codonopsis pilosula, rhizoma atractylodis macrocephalae, poria and liquorice powder in preparing medicament for improving immunity of rabbits. According to the application, the codonopsis pilosula, rhizoma atractylodis macrocephalae, poria and liquorice powder accounting for 1-5 percent of the weight of basic ration is added into the basic ration, the basic ration is fed to rabbits, and the rabbit sacculus rotundus index, thymus index, spleen index or dendritic index of a rabbit can be increased, the immunity of the rabbit can be improved, the expression quantity of an antifungal protein gene can be increased, and theoretical direction is provided to the codonopsis pilosula, rhizoma atractylodis macrocephalae, poria and liquorice powder in improving rabbit immunity.

The invention relates to a class of antibacterial cationic reactive dyes and a preparation method thereof. The dye matrix is ​​an anthraquinone structure, the active group is a chloro-s-triazine or a fluoro-s-triazine, and the water-soluble group is a quaternary ammonium salt structure with a long carbon chain. The chemical structure is as follows: This type of dye can be used for cellulose fibers , protein fiber, polyamide fiber, acrylic fiber, cationic dyeable polyester fiber dyeing and antibacterial finishing, with high antibacterial effect, excellent color fastness, antibacterial durability. The preparation method of the dye includes: a. dissolving aminoanthraquinone A and trichloro or trifluoro-s-triazine in a nitrobenzene organic solvent, heating to 90°C, rising to 120°C after 1 hour, cooling and filtering to obtain the dye intermediate B; b. Dissolve the dye intermediate B prepared in step a in DMF organic solvent, add tertiary amine intermediate C, react at 55°C for 3-4 hours, evaporate the solvent DMF to obtain dye intermediate D, and dry it in vacuum c. Dissolve the dye intermediate D obtained in step b in DMF organic solvent, add haloalkyl E, heat to about 120°C, react for 4-5 hours, evaporate the solvent DMF, wash with ether, and recrystallize with ether-ethanol , Dye F was obtained and dried in vacuum. When this type of dye is used for textile dyeing, it can also endow textiles with certain functions, and has the advantages of water saving, energy saving, and ecological protection.

The invention discloses a nucleic acid sequence of a pteromalus puparum antifungal protein Pp-AP3 code. The separated DNA molecule comprises: a polypeptide nucleotide sequence with the pteromalus puparum antifungal protein Pp-AP3 activity is encoded, the nucleotide sequence and the nucleotide sequence from the 767th ribonucleotide to the 871th ribonucleotide in the SEQ ID NO:1 have 70 percent of homology; or the nucleotide sequence can be hybridized with the nucleotide sequence from the 767th ribonucleotide to the 871th ribonucleotide in the SEQ ID NO:1 at a temperature of between 40 and 55 DEG C. The invention also discloses a pteromalus puparum antifungal protein polypeptide Pp-AP3 which has an amino acid sequence shown in the SEQ ID NO: 2. The pteromalus puparum antifungal protein Pp-AP3 has the antibiosis performance.

The invention provides an application of anthraquinone compound in preparing an antifungal drug and an antifungal composition, which belongs to the technical field of medicine. The anthraquinone compound is 9,10-dioxo-N-(5-phenylthiazole-2-groupyl)-9,10-dihydroanthracene-2-sulfonyl; and an in vitro cell experiment proves that the anthraquinone compound has good antifungal effect for various clinical fungi strains, the application dosage of the antifungal drug can be obviously reduced, the inhibition effect for the drug-resistant fungi can be improved, and the effect of the antifungal drug for the drug-resistant fungi can be restored.