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Antifungal protein separated and purified from bacillus subtilis J18, protein product, preparation method and application

A technology of Bacillus subtilis and antibacterial protein, which is applied in the field of antibacterial protein, can solve the problems of strict storage environment requirements, short validity period, and susceptibility to ultraviolet rays, and achieve the effect of wide application range and delaying fruit aging

Inactive Publication Date: 2013-04-17
BIOLOGY INST OF HEBEI ACAD OF SCI
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Problems solved by technology

At present, the biological pesticides on the market are mainly live bacterial biological preparations. When they are used, they often have slow effect, unstable control effect, and are easily affected by ultraviolet rays. In addition, they have a short validity period during storage, which is harmful to the storage environment. strict requirements

Method used

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Embodiment Construction

[0032] The present invention will be further described below in conjunction with embodiment.

[0033] The strain number of the Bacillus subtilis J18 is CGMCC No.3665, and the strain name is Bacillus subtilis J18.

[0034] A kind of preparation method of the antibacterial protein isolated and purified by Bacillus subtilis J18 of the present invention, it comprises the following steps;

[0035] Preparation of J18 antibacterial protein;

[0036] Seed activation: transfer the Bacillus subtilis J18 strain from the preserved glycerol bacteria to LB liquid medium (the components are: tryptone 10g, yeast extract 5g, sodium chloride 10g, water 1000mL), at 30℃, Cultivate at 150-250 rpm for 18 hours to obtain J18 bacterial suspension. Take the cultured bacterial suspension and dilute it, evenly spread it on LB solid plate medium (tryptone 10g, yeast extract 5g, sodium chloride 10g, agar 15g, water 1000mL), after 48h incubator at 25℃, use Pick a single colony with a toothpick and inocu...

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Abstract

The invention relates to an antifungal protein separated and purified from bacillus subtilis J18, a protein product, a preparation method and an application. A strain serial number of the bacillus subtilis J18 is CGMCC No.3665, and a strain name is the bacillus substilis J18. The preparation method of the protein product comprises the following steps of: mixing the J18 antifungal protein with 1 percent of acetic acid to form a chitosan solution with a pH value of 6.0, compositing the chitosan solution to form a film coating reagent which has the concentration of 0.05mg / mL and is used for soaking a fruit, and also coating a mixture which is formed by mixing the J18 antifungal protein solution with an oxidized starch solution on a preservative paper to prepare an antifungal preservative paper used for wrapping the fruit. The antifungal protein has remarkable control effect to fruit and crop diseases and particularly black spot diseases of pears after picking and is an antagonistic strain with broad spectrum and high efficiency.

Description

technical field [0001] The invention relates to an antibacterial protein isolated and purified from bacillus subtilis J18, protein product, preparation method and application. Background technique [0002] At present, the post-harvest rot loss of fruit in our country is serious, resulting in huge economic losses. The infection of postharvest pathogenic microorganisms is the most important cause of postharvest rot loss. However, the extensive use of chemical fungicides in fruit preservation has greatly affected the food safety of fruits. How to reduce the use of chemical fungicides in fruit preservation has become a food safety issue that the public is very concerned about. [0003] Bacillus subtilis (Ehrenberg) Cohn is a common non-pathogenic bacterium in nature, which can secrete a variety of antibacterial substances. It has the advantages of non-toxic and harmless to humans and animals, and does not produce drug resistance. It has broad application value in the research ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/02C07K1/30A23B7/16D21H21/14A01N63/02A01P3/00C12R1/125
Inventor 宋水山宋聪贾振华黄亚丽马宏李惠荣
Owner BIOLOGY INST OF HEBEI ACAD OF SCI
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