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The application provides a nanocellulose-based edible preservative liquid, a preparation method and a use method thereof. The nanocellulose-based edible preservative liquid comprises a nanocellulose solution, beeswax and glycerol. The preparation method comprises the following steps: mixing the nanocellulose solution, the beeswax and the glycerol according to a proportion and heating to melt the beeswax to obtain a mixed liquid; then, stirring and emulsifying the mixed liquid to obtain a nanocellulose emulsion; finally, performing vacuum defoaming treatment on the nanocellulose emulsion to obtain the nanocellulose-based edible preservative liquid. The application develops an edible and easy-to-wash preservative liquid based on nanocellulose, adopts a dipping coating mode to perform coating film treatment on fruits and vegetables, forms a micro-nano level preservative coating layer on the surface of the fruits and vegetables, can effectively reduce the loss of water and nutrients on the surface and inside of the fruits and vegetables, reduces the gas permeability of the preservative coating layer, and significantly enhances the mechanical strength of the preservative coating layer.