44results about How to "High purification factor" patented technology

The invention belongs to the technical field of nuclear fuel cycles, and discloses a method for coextracting uranium, plutonium and neptunium. The method is implemented in a way that: neptunium is coextracted with uranium and plutonium at the co-decontamination section, so that the neptunium enters an organic phase 1AP. The key point is to oxidate Np (V) in an extraction apparatus 1A into Np (VI) which can be extracted by the extraction apparatus. The method implements high yields of uraniumm, plutonium and neptunium, does not increase the salt content in the after-treatment process, and can implement quantitative extraction of the uranium, plutonium and neptunium.

The invention belongs to the field of deep processing of garlic, and particularly discloses a method for purifying alliinase by double-water-phase separation. The method comprises the following steps: (1) peeling and precooling garlic squamous bulbs, immersing in a precooled extract, homogenizing, centrifuging, and discarding garlic slag, thereby obtaining the supernate crude enzyme solution; (2) dissolving ammonium sulfate, sodium dihydrogen phosphate or trisodium citrate in a Britton-Robinson buffer solution, oscillating to completely dissolve, adding a PEG (polyethylene glycol) solution, evenly mixing by oscillation, and standing for phase splitting, thereby obtaining a double-water-phase extractant; (3) adding the crude enzyme solution into the double-water-phase extractant, oscillating, centrifuging, and standing for phase splitting; and (4) ultrafiltering the lower phase of the enriched alliinase, and carrying out vacuum freeze drying on the trapped fluid to obtain the alliinase solid. The method is simple to operate, has the advantages of simple apparatuses, time saving, low cost, and higher alliinase purification multiple and enzyme activity recovery rate than the prior art, and can easily implement scale-up production.

The invention discloses a method for separating and purifying a protease capable of degrading soybean protein allergens, belonging to the technical field of protein purification. The method comprises the following step: separating and purifying the protease which is sourced from a wild strain Bacillus sp.BBE201108 and has the effect of degrading soybean protein allergens by sequentially adopting ammonium sulfate distributed precipitation, dialysis, anion exchange chromatography (Q FF) and gel filtration chromatography (Superdex 75). The protease disclosed by the invention can be successfully prepared by virtue of separation and purification, and a foundation for analyzing enzymatic properties and amino acid sequences of the protease and achieving heterologous expression research of the protease can be provided.

The invention discloses a method for extracting lactoperoxidase by the ultrafiltration assisted two aqueous phase extraction technology. The method comprises the steps of using bovine whey as the raw material; enriching all lactoperoxidase from bovine whey into a salt phase by the two aqueous phase extraction technology; processing the salt phase solution through an ultrafiltration membrane to remove small impure protein; further purifying lactoperoxidase; desalting through a dialysis bag; then freezing and drying, so as to prepare a high-activity lactoperoxidase product which can be used in natural food additives. With the adoption of the method, lactoperoxidase with natural antibacterial activity can be directly prepared and directly added as a preservative into foods or toothpaste and other daily supplies.

The invention relates to a preparing method and the application of a nylon affinity membrane which takes Reactive Blue 4 as a petunidin. The preparation includes: the nylon membrane after being activated is modified by chitosan; the modified nylon membrane is dipped into a Reactive Blue 4 dye and added with NaCL solution after heat preservation to react, added with Na2CO3 solution for reacting continuously after temperature rising, respectively washed by hot de-ionized water, methanol solution, NaCl solution, urea solution and de-ionized water to obtain the nylon affinity membrane which takes Reactive Blue 4 as a petunidin. The application includes that pawpaw powders are sampled on the nylon affinity membrane which takes Reactive Blue 4 as a petunidin, washed by Tris-HCL buffer solution and eluted by NaSCN solution to obtain pawpaw prolease; the activity of the prolease and the protein content are tested; the purification times are calculated. The method is fast, simple and convenient and has large separation capacity; the prolease extracted has the advantages of good activity, high purity as well as being suitable for scale production.

The invention relates to the technical field of biosorbent preparation and application, and specifically discloses a modified beer waste yeast adsorbent, a preparation method and an application thereof. The modified beer waste yeast adsorbent provided by the present invention is a novel adsorbent which is prepared from a cross-linked beer waste yeast modified through citric acid, and provides good absorbability for lysozyme such that the modified beer waste yeast adsorbent can be applicable for extracting the lysozyme.

The invention provides a purification method of acetylcholinesterase from earthworm serum. The method comprises the following steps: placing earthworm in clean water for 24 hours to eliminate excrement in the earthworm body, and storing the earthworm in a refrigerator for later use; homogenizing with phosphoric acid buffer solution in an ice-water bath condition to prepare homogenate, centrifuging to obtain crude enzyme extract, and storing in the refrigerator; adding surfactant Triton X-114 solution and citric acid buffer solution into 100mu L of crude extract, and diluting with secondary water until the volume is 10mL, sufficiently oscillating and shaking uniformly; placing the solution in a constant-temperature water bath with the water bath temperature of 35 DEG C to balance for 9 minutes; and centrifuging for layering to obtain a lower product which is the purified acetylcholinesterase product. The purification extraction method of the acetylcholinesterase from earthworm serum has wide enzyme sources and low price, high sensitivity, simple and stable preparation process and low production cost.

The invention provides an antifungal protein, which is obtained by purifying a water soluble extract of hulless oat seed, wherein the apparent molecular weight of the antifungal protein is about 35kDa. The preparation method comprises the following steps: crushing and degreasing the hulless oat seed; extracting the hulless oat seed by buffer solution to obtain a coarse protein; and obtaining the antifungal protein by the two-step chromatographic separation which is the Resource S cation exchange and Superdex 75 gel exclusion chromatographic purification. The antifungal protein has obvious effect of inhibiting the growth of white rot fungus and trichoderma viride, and the minimum inhibition for the white rot fungus is 0.42mu g, and 0.21mu g for the thetrichoderma viride. In addition, the antifungal protein also has certain effect of inhibiting the growth of fusarium. The antifungal protein can be used as a food anticorrosion additive, a crop antifungal damage spraying agent and the like, and has high practical values in the aspects of food anticorrosion, crop prophylaxis and crop disease resistance.

The invention belongs to the technical field of spent fuel post-treatment, and discloses a process for purifying zirconium in a Purex flow plutonium purification cycle. 30% tri-butyl-phosphate (TBP)-kerosene is adopted as an extraction agent 2AX in a 2A extractor, so as to recover uranium and plutonium and purify fission fragments. The process comprises an extraction section and a washing section; and the concentration of a nitric acid in a 2AF feed liquid is 3.5-3.7mol/L, and 2AS is an HNO3 solution of which the concentration is 0.8-0.85mol/L. The process has the advantages that the plutonium yield is greater than 99.9%, and the purification coefficient of zirconium is greater than 100.

The invention provides a method for purifying a hyaluronidase by an affinity membrane chromatography, which uses a nylon membrane as a matrix, a method of bonding chitosan is used to modify the nylonmembrane, so that the non-specific adsorption of the membrane is greatly reduced, and a novel dye affinity membrane is obtained, which has good adsorption to hyaluronidase and has a high purificationratio.

The invention provides a preparation method of an antitussive, expectorant, anti-inflammatory and anti-microbial extract from earthworms. The extraction method provided by the invention mainly extracts the earthwormhomogenate through NaAC buffer and acetone of pH 4.8-5.4. The invention optimizes the extraction process provided by the applicant through repeated experiments, greatly shortens the preparation time of earthworm extract, and reduces the preparation time of earthworm extract from 42to 45 hours to 15 to18 hours; the yield was increased from 73% to 82%, and the recovery of total activity was increased from 1141 to 4443, and the specific activity was increased by 3.9 times. The purification times increased from 41 times to 158 times, and the purification times of the active component of deoxyribonuclease increased nearly fourfold. The preparation method provided by the invention is simple in process, low in cost and easy to be popularized and applied in industrial production. The purity and the activity of the product prepared by the preparation method provided by the invention are significantly higher than those of the related similar products prepared by the prior art.

The invention provides recombinant beta-galactosidase and a construction method and application thereof, and belongs to the technical field of bioengineering. The recombinant beta-galactosidase beta-Gal-LEH is constructed in the invention, and beta-Gal and ELP (VPGVG) 50 are connected to construct the recombinant beta-galactosidase beta-Gal-LEH which can be self-purified and has good stability andhigh lactose hydrolysis efficiency.

The invention discloses a method for extracting lipoxygenase from soybean whey waste water through inverse pH gradient, and belongs to the technical field of utilization of waste water obtained duringfood processing. The method comprises the following steps of after the soybean whey waste water is pretreated, extracting the lipoxygenase by an inverse pH gradient method, firstly removing hybridprotein at the pH of isoelectric points of the lipoxygenase, then separating the lipoxygenase at the isoelectric points of the lipoxygenase, and then combining ultrafiltration with gel chromatography toobtain refined lipoxygenase. The soybean whey waste water is used for obtaining the lipoxygenase high in industrial value, and the extraction rate of the lipoxygenase is high. The lipoxygenase is highin purity and good in enzymatic activity. The method is convenient to operate, safe, effective, and low in cost, and can be used for development and utilization of the lipoxygenase in the soybean whey waste water in industrial use so as to realize source recycling. The method has notable economic benefits and social benefits.

The invention discloses a movable radioactive waste water far infrared treatment device. The device comprises a transport cart, a heat-preserving cabin, a waste water treatment system, an automatic control device and an external pipeline. The heat-preserving cabin is arranged on the transportation cart. The waste water treatment system and the automatic control device are arranged in the heat-preserving cabin. The automatic control device is used for controlling the operation of the waste water treatment system. Radioactive waste water high in salt content and oil content can be treated, a purification coefficient is high, a safety coefficient is high, the device is small and high in mobility and applicability, and the technical effect of reducing pollution dispersal is achieved.

The invention discloses a purification method of malt limit dextrinase. The method includes: 1) Ammonium sulfate step-by-step precipitation: add ammonium sulfate with a saturation of 25-35% to the enzyme crude extract Ⅰ to remove impurity proteins, and then add ammonium sulfate to the supernatant to a saturation of 75-35%. 85%, salting out to get the crude enzyme solution II; 2) Ion exchange chromatography: After the filler is pretreated and packed, equilibrate with 15~20mmol/LTris-HCl buffer solution with a pH value of 7.0, load the sample, and then use 0~ Gradient elution with 1mol/L NaCl buffer solution; 3) Gel filtration chromatography: After the sample separated by ion exchange chromatography column is further purified by gel filtration chromatography column, it is freeze-dried to obtain a highly purified limit dextrinase product . The method has the advantages of simple process, safety, low cost, high product purity and good purification effect, and is an effective and reliable method for purifying limit dextrinase.

The invention belongs to the technical field of biological enzymes, and particularly relates to a method for purifying xylanase. The method disclosed by the invention takes coarse xylanase powder which is sold in the market as a raw material, and the raw materials can be produced through steps, including pH regulation, hollow fibrous membrane filtering, ultrafiltration, freeze drying and the like,to obtain purified xylanase. By use of the method disclosed by the invention, ultrafiltration stage treatment is adopted, a purification multiple and efficiency can be effectively improved, production cost is lowered, in addition, liquid nitrogen can be adopted to realize quick cryopreserving of enzyme liquid, enzyme crystal structure change and enzyme activity loss caused by slow pre-freezing can be reduced, and product performance is improved. The method disclosed by the invention has the characteristics of a simple production technology, convenience in production, a high product yield, a low equipment investment and operation cost, a high resource comprehensive utilization rate and the like. The xylanase product purified by the method disclosed by the invention has high activity, can meet the requirement of the medicine industry for high-purity enzyme preparations, and can be widely applied to industries, including medicines, food, chemical engineering and the like.